Recombinant Mouse FGFR5 Fc Chimera Protein, CF

Catalog #: 1899-FR Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
1899-FR-050

Recombinant Mouse FGFR5 Fc Chimera Protein Binding Activity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse FGFR5 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA.

200 ng/mL of biotinylated Recombinant Mouse FGF1 is mixed with serially diluted Recombinant Mouse FGF R5 Fc Chimera. Following incubation, the FGF R5-FGF1/biotin complex is captured on an EvenCoat^TM Streptavidin Microplate (Catalog # CP003). Bound FGF R5 is measured using Rt x mFGF R5 followed by HRP labeled Gt x Rat IgG. The concentration of Recombinant Mouse FGF R5 Fc Chimera that produces 50% of the optimal binding is 30-120 ng/mL.

Source

Mouse myeloma cell line, NS0-derived mouse FGF R5/FGFRL1 protein

Mouse FGF R5 (Ala21-Pro374) Accession # NP_473412	PIEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

NP_473412

N-terminal Sequence
Analysis

Ala21

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

65.6 kDa (monomer)

SDS-PAGE

85-95 kDa, reducing conditions

Product Datasheets

1899-FR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1899-FR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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Recombinant Mouse FGF R5 Fc Chimera (Catalog # 1899-FR) binds biotinylated Recombinant Mouse FGF1. The concentration of Recombinant Mouse FGF R5 Fc Chimera that produces 50% of the optimal binding response is 30-120 ng/mL.

Reconstitution Calculator

Background: FGFR5/FGFRL1

Fibroblast growth factor receptor 5 (FGF R5), also known as FGRL1, is a 65 kDa transmembrane member of the FGF receptor family (1). Mature mouse FGF R5 consists of a 354 amino acid (aa) extracellular domain (ECD) with three immunoglobulin-like domains, a 21 aa transmembrane segment, and a 134 aa cytoplasmic domain (2). FGF R5 is distinct from other FGF receptor family members in that it does not contain a cytoplasmic tyrosine kinase domain (2, 3). Within the ECD, mouse FGF R5 shares 94% and 98% aa sequence identity with human and rat FGF R5, respectively. Alternate splicing results in an isoform that lacks the first Ig-like domain (2). FGF R5 is widely expressed, and expression of two species of mRNA in cartilage and pancreas may indicate the presence of both splice forms (2 - 6). Both the full length and truncated isoforms of FGF R5 bind FGF basic; the full length form additionally binds to heparin (2, 6). FGF R5 may function as a decoy receptor by binding FGF but not transducing its mitogenic signals (6).

References

Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
Sleeman, M. et al. (2001) Gene 271:171.
Kim, I. et al. (2001) Biochim. Biophys. Acta 1518:152.
Antoine, M. et al. (2005) Growth Factors 23:87.
Wiedemann, M. et al. (2000) Genomics 69:275.
Trueb, B. et al. (2003) J. Biol. Chem. 278:33857.

Long Name

Fibroblast Growth Factor Receptor-like 1

Entrez Gene IDs

53834 (Human); 116701 (Mouse)

Alternate Names

FGF homologous factor receptor; FGF R5; FGF receptor-like protein 1; FGFR5; FGFR-5; FGFRL1; FGFR-like protein; FHFR; Fibroblast growth factor receptor 5; fibroblast growth factor receptor-like 1