Recombinant Mouse Frizzled-5 Fc Chimera Protein, CF

Catalog #: 9060-FZ Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9060-FZ-050

Recombinant Mouse Frizzled-5 Fc Chimera Protein Bioactivity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Frizzled-5 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED₅₀ for this effect is 10-60 ng/ml in the presence of 5 ng/mL of Recombinant Mouse Wnt-3a (Catalog # 1324-WN).

Source

Mouse myeloma cell line, NS0-derived mouse Frizzled-5 protein

Mouse Frizzled-5 (Ala27-Pro167) Accession # Q9EQD0	IEGRMD	Mouse IgG_2a (Glu98-Lys330)
N-terminus		C-terminus

Accession #

Q9EQD0

N-terminal Sequence
Analysis

Ala27

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

43 kDa

SDS-PAGE

54-64 kDa, reducing conditions

Product Datasheets

9060-FZ

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9060-FZ

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant Mouse Frizzled-5 FC Chimera (Catalog # 9060-FZ) inhibits Recombinant Mouse Wnt-3a (Catalog # 1324-WN) induced alkaline phosphatase production in the MC3T3-E1 mouse preosteoblast cell line. The ED₅₀ for this effect is 0.01-0.06 µg/ml.

Reconstitution Calculator

Background: Frizzled-5

Frizzled-5 is an approximately 62 kDa 7-TM glycoprotein that is one of at least ten Frizzled family Wnt receptors (1, 2). Wnt engagement of Frizzled, with low density lipoprotein receptor-related proteins LRP-5 or LRP-6 acting as co‑receptors, stabilizes beta -catenin and promotes gene transcription that is important in development and tissue maintenance (1). Frizzleds can also signal through non-canonical pathways independently of LRPs (1). Mature mouse Frizzled-5 consists of a 212 amino acid (aa) extracellular domain (ECD), a 283 aa 7-TM region, and a 64 aa cytoplasmic domain with a PDZ binding motif (2, 3). The ECD includes a cysteine-rich region (CRD) that binds Wnts and is highly conserved among Frizzled proteins (1, 2, 4). Within the N-terminal ECD, human Frizzled-5 shares 95% aa sequence identity with mouse and rat Frizzled-5. Frizzled-5 is expressed in embryonic tissues (telencephalon, pituitary, thalamus, hypothalamus, eye, liver, spleen, lung, and kidney), in the adult retina, colon, and pancreatic islets, some cancer cell lines, human embryonic stem cells, and in some monocyte and lymphocyte populations (2-8). Frizzled-5 functions as a receptor for Wnt-5a, Wnt-9b, Wnt-10b, Wnt-2b, and Wnt-7a (3, 5, 9, 10). It plays a role in the maintenance of yolk sac and placental vasculature (ishikawa) and in the regression of vitreous vasculature during eye development (5, 6, 11). It mediates the synaptogenic effect of Wnt-7a, contributes to the development of neuronal polarity, and is required for neuronal survival in the thalamus (3, 12, 13). In macrophages and monocytes, Frizzled-5 signaling induces the production of inflammatory cytokines (7, 8).

References

Schulte, G. (2015) Eur. J. Pharmacol. 763:191.
Saitoh T. et al. (2001) Int. J. Oncol. 19:105.
Liu, C. et al. (2008) J. Neurosci. 28:5641.
Katoh, Y. and M. Katoh (2007) Int. J. Oncol. 30:751.
Ishikawa, T. et al. (2001) Development 128:25.
Burns, C.J. et al. (2008) Dev. Dyn. 237:1614.
Periera, C. et al. (2008) Arterioscler. Thromb. Vasc. Biol. 28:504.
Blumenthal, A. et al. (2006) Blood 108:965.
He, X. et al. (1997) Science 275:1652.
Caricasole, A. et al. (2003) J. Biol. Chem 278:37024.
Zhang, J. et al. (2008) Invest. Ophthalmol. Vis. Sci. 49:5561.
Sahores, M. et al. (2010) Development 137:2215.
Slater, P.G. et al. (2013) PLoS One 8:e78892.

Entrez Gene IDs

7855 (Human); 14367 (Mouse)

Alternate Names

C2orf31; chromosome 2 open reading frame 31; DKFZP434E2135; frizzled (Drosophila) homolog 5; frizzled homolog 5 (Drosophila); Frizzled5; Frizzled-5; Frz-5; FZ-5; FZD5; fzE5; HFZ5; MGC129692; seven-transmembrane receptor frizzled-5; Wnt receptor