Recombinant Mouse Frizzled-7 Fc Chimera Protein, CF

Catalog #: 198-FZ
2 Citations Datasheet / COA / SDS

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198-FZ-050

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Frizzled-7 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to bind biotinylated Wnt-3a in a functional ELISA with an estimated K_D < 5 nM.

Source

Mouse myeloma cell line, NS0-derived mouse Frizzled-7 protein

Mouse Frizzled-7 (Gln33 - Leu185) Accession # Q61090	IEGRMD	Human IgG₁ (Pro100 - Lys330)
N-terminus		C-terminus

Accession #

Q61090

N-terminal Sequence
Analysis

No results obtained: Gln33 predicted

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

43 kDa (monomer)

SDS-PAGE

55-60 kDa, reducing conditions

Product Datasheets

198-FZ

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

198-FZ

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 200 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Frizzled-7

Frizzled-7 is a member of the Frizzled family of unconventional G-protein-coupled glycoprotein receptors for the Wnt signaling pathway (1 ‑ 3). The Wnt genes encode a large family of glycoproteins that are essential in development and tissue maintenance (1, 2). Like other Frizzled family members, mouse Frizzled-7 contains a divergent N‑terminal signal peptide (amino acid (aa) 1 ‑ 32), a highly conserved extracellular cysteine-rich domain (CRD, aa 44 ‑ 169), a variable-length linker region (aa 170 ‑ 254), a seven-pass transmembrane region (aa 255 ‑ 547), and a variable-length C-terminal cytoplasmic domain (aa 548 ‑ 572) (1 ‑ 4). The CRD, which comprises the binding site for Wnts and other ligands such as Syndecan 4 and fibronectin, spans about 130 amino acid residues and contains ten invariant cysteine residues (2 ‑ 4). Expressed alone, it can compete with native Frizzled to inhibit Wnt canonical signaling (5). Within aa 33 ‑ 185, mouse Frizzled-7 shares ~99% aa identity with human, rat, canine and bovine Frizzled-7. Mature Frizzled-7 also shares 80% aa identity with Frizzled-1 and Frizzled-2. Roles for Frizzled-7 have been determined in both canonical Wnt/ beta -Catenin-mediated signaling and non‑canonical planar cell polarity and calcium pathways (1, 2, 5). During development, Frizzled-7 is expressed during gastrulation and in the fetal gut, kidney and lung where it is thought to influence tissue morphogenesis via non‑canonical signaling pathways (3 ‑ 6). In the adult, Frizzled-7 is expressed in skeletal muscle, especially in satellite cells that mediate muscle regeneration in response to Wnt7a (3, 4, 7). It is expressed in embryonic stem cells (ES), contributing to self-renewal signaling (8). It has been implicated in mesenchymal-to-epithelial transition in colorectal cancer (2, 9). Frizzled-7 mRNA has also been detected in adult heart and placenta (4).

References

Schulte, G. and V. Bryja (2007) Trends Pharmacol. Sci. 28:518.
Vincan, E. et al. (2007) Front. Biosci. 12:4558.
Wang, Y. et al. (1996) J. Biol. Chem. 271:4468.
Sagara, N. et al. (1998) Biochem. Biophys. Res. Commun. 252:117.
Medina, A. et al. (2000) Mech. Dev. 92:227.
Kemp, C.R. et al. (2007) Dev. Dyn. 236:2011.
Le Grand, F. et al. (2009) Cell Stem Cell 4:535.
Melchior, K. et al. (2008) Biol. Chem. 389:897.
Vincan, E. et al. (2007) Oncogene 26:2340.

Entrez Gene IDs

8324 (Human); 14369 (Mouse)

Alternate Names

frizzled (Drosophila) homolog 7; frizzled homolog 7 (Drosophila); Frizzled, drosophila, homolog of, 7; Frizzled7; Frizzled-7; fz-7; FZD7; FzE3frizzled-7; hFz7

Related Research Areas

Citations for Recombinant Mouse Frizzled-7 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Survival, neuron-like differentiation and functionality of mesenchymal stem cells in neurotoxic environment: the critical role of erythropoietin.
Authors: Danielyan L, Schafer R, Schulz A, Ladewig T, Lourhmati A, Buadze M, Schmitt AL, Verleysdonk S, Kabisch D, Koeppen K, Siegel G, Proksch B, Kluba T, Eckert A, Kohle C, Schoneberg T, Northoff H, Schwab M, Gleiter CH
Cell Death Differ., 2009-07-17;16(12):1599-614.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Canonical Wnt signaling is required for development of embryonic stem cell-derived mesoderm.
Authors: Lindsley RC, Gill JG, Kyba M, Murphy TL, Murphy KM
Development, 2006-08-30;133(19):3787-96.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay