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Recombinant Mouse G-CSF Protein

Catalog #: 414-CS
20 Citations 3 Reviews Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
414-CS-005/CF
414-CS-025/CF

With Carrier

Catalog # Availability Size / Price Qty
414-CS-005
414-CS-025
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (20)
FAQs
Reviews (3)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse G-CSF Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using NFS‑60 mouse myelogenous leukemia lymphoblast cells. Shirafuji, N.et al. (1989) Exp. Hematol. 17:116. The ED50 for this effect is 10-60 pg/mL.
Source
E. coli-derived mouse G-CSF protein
Val31-Ala208, with an N-terminal Met
Accession #
P09920
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
19 kDa

Product Datasheets

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414-CS (with carrier)

Product Datasheet
COA
SDS

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414-CS/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

414-CS

Formulation Lyophilized from a 0.2 μm filtered solution in Citric Acid and Tween® 20 with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile deionized water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

414-CS/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Citric Acid and Tween® 20 with Trehalose.
Reconstitution Reconstitute at 100 μg/mL in sterile deionized water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: G-CSF

G-CSF is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNF-alpha and IFN-gamma. Other cell types including fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells can also secrete G-CSF after LPS, IL-1 or TNF-alpha activation. In addition, various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively.

The murine G-CSF cDNA encodes a 208 amino acid (aa) residue precursor protein containing a 30 aa residue signal peptide that is proteolytically cleaved to generate the 178 aa residue mature protein. Human G-CSF is 73% identical at the amino acid level to murine G-CSF and the two proteins show species cross-reactivity.

In vitro, G-CSF stimulates growth, differentiation and functions of cells from the neutrophil lineage. It also has blast cell growth factor activity and can synergize with IL-3 to shorten the Go period of early hematopoietic progenitors. Consistent with its in vitro functions, G-CSF has been found to play important roles in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis. Recombinant human G-CSF has been approved for the amelioration of chemotherapy induced neutropenia as well as for severe chronic neutropenia following marrow transplant.

Long Name
Granulocyte Colony Stimulating Factor
Entrez Gene IDs
1440 (Human); 12985 (Mouse)
Alternate Names
C17orf33; chromosome 17 open reading frame 33; colony stimulating factor 3 (granulocyte); CSF3; CSF3OS; Filgrastim; GCSF; G-CSF; GCSFlenograstim; granulocyte colony-stimulating factor; Lenograstim; MGC45931; Pluripoietin

Citations for Recombinant Mouse G-CSF Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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  1. MerTK Induces Dysfunctional Dendritic Cells by Metabolic Reprogramming
    Authors: Zewdie, EY;Edwards, GM;Hunter, DM;Earp, HS;Holtzhausen, A;
    Cancer immunology research
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Phenotypic screening of signaling motifs that efficiently induce cell proliferation
    Authors: Umene, K;Nagamune, T;Kawahara, M;
    Scientific reports
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Zinc Finger Protein St18 Protects against Septic Death by Inhibiting VEGF-A from Macrophages
    Authors: K Maruyama, H Kidoya, N Takemura, E Sugisawa, O Takeuchi, T Kondo, MMA Eid, H Tanaka, MM Martino, N Takakura, Y Takayama, S Akira, A Vandenbon, Y Kumagai
    Cell Rep, 2020-07-14;32(2):107906.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Neutrophils are essential for induction of vaccine-like effects by antiviral monoclonal antibody immunotherapies
    Authors: M Naranjo-Go, J Lambour, M Piechaczyk, M Pelegrin
    JCI Insight, 2018-05-03;3(9):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. A Bach2-Cebp Gene Regulatory Network for the Commitment of Multipotent Hematopoietic Progenitors
    Authors: A Itoh-Nakad, M Matsumoto, H Kato, J Sasaki, Y Uehara, Y Sato, R Ebina-Shib, M Morooka, R Funayama, K Nakayama, K Ochiai, A Muto, K Igarashi
    Cell Rep, 2017-03-07;18(10):2401-2414.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Therapeutic Targeting of the G-CSF Receptor Reduces Neutrophil Trafficking and Joint Inflammation in Antibody-Mediated Inflammatory Arthritis
    Authors: Arna E Andrews
    J. Immunol., 2016-11-02;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Active enhancers are delineated de novo during hematopoiesis, with limited lineage fidelity among specified primary blood cells.
    Authors: Luyten A, Zang C, Liu X, Shivdasani R
    Genes Dev, 2014-08-15;28(16):1827-39.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Granulocyte-colony stimulating factor attenuates oligomeric amyloid beta neurotoxicity by activation of neprilysin.
    Authors: Doi Y, Takeuchi H, Mizoguchi H, Fukumoto K, Horiuchi H, Jin S, Kawanokuchi J, Parajuli B, Sonobe Y, Mizuno T, Suzumura A
    PLoS ONE, 2014-07-25;9(7):e103458.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay, ICC
  9. Macrophage-inducing FasL on chondrocytes forms immune privilege in cartilage tissue engineering, enhancing in vivo regeneration.
    Authors: Fujihara Y, Takato T, Hoshi K
    Stem Cells, 2014-05-01;32(5):1208-19.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  10. G-CSF drives a posttraumatic immune program that protects the host from infection.
    Authors: Gardner J, Noel J, Nikolaidis N, Karns R, Aronow B, Ogle C, McCormack F
    J Immunol, 2014-01-27;192(5):2405-17.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  11. Aged mice are unable to mount an effective myeloid response to sepsis.
    Authors: Nacionales D, Gentile L, Vanzant E, Lopez M, Cuenca A, Cuenca A, Ungaro R, Li Y, Baslanti T, Bihorac A, Moore F, Baker H, Leeuwenburgh C, Moldawer L, Efron P
    J Immunol, 2013-12-13;192(2):612-22.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Identification of transcription factors for lineage-specific ESC differentiation.
    Authors: Yamamizu K, Piao Y, Sharov A, Zsiros V, Yu H, Nakazawa K, Schlessinger D, Ko M
    Stem Cell Reports, 2013-11-27;1(6):545-59.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  13. A soluble form of LMIR5/CD300b amplifies lipopolysaccharide-induced lethal inflammation in sepsis.
    J. Immunol., 2012-07-06;189(4):1773-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Smad1 signaling restricts hematopoietic potential after promoting hemangioblast commitment.
    Authors: Cook BD, Liu S, Evans T
    Blood, 2011-04-22;117(24):6489-97.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  15. G-CSF promotes the proliferation of developing cardiomyocytes in vivo and in derivation from ESCs and iPSCs.
    Authors: Shimoji K, Yuasa S, Onizuka T, Hattori F, Tanaka T, Hara M, Ohno Y, Chen H, Egasgira T, Seki T, Yae K, Koshimizu U, Ogawa S, Fukuda K
    Cell Stem Cell, 2010-03-05;6(3):227-37.
    Species: Mouse
    Sample Types: In Vivo, Whole Cells
    Applications: Bioassay, In Vivo
  16. Bv8 regulates myeloid-cell-dependent tumour angiogenesis.
    Authors: Shojaei F, Wu X, Zhong C, Yu L, Liang XH, Yao J, Blanchard D, Bais C, Peale FV, van Bruggen N, Ho C, Ross J, Tan M, Carano RA, Meng YG, Ferrara N
    Nature, 2007-12-06;450(7171):825-31.
    Species: Mouse
    Sample Types: In Vivo, Whole Cells
    Applications: Bioassay, In Vivo
  17. Differential requirements for hematopoietic commitment between human and rhesus embryonic stem cells.
    Authors: Rajesh</LastName><ForeNam D</Initial, Rajesh D, Chinnasamy N, Mitalipov SM, Wolf DP, Slukvin I, Thomson JA, Shaaban AF
    Stem Cells, 2007-02-01;25(2):490-9.
    Species: Human, Mouse, Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Bone marrow-derived cells require a functional glucose 6-phosphate transporter for normal myeloid functions.
    Authors: Kim SY, Nguyen AD, Gao JL, Murphy PM, Mansfield BC, Chou JY
    J. Biol. Chem., 2006-08-04;281(39):28794-801.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  19. Recruitment of stem and progenitor cells from the bone marrow niche requires MMP-9 mediated release of kit-ligand.
    Authors: Heissig B, Hattori K, Dias S, Friedrich M, Ferris B, Hackett NR, Crystal RG, Besmer P, Lyden D, Moore MA, Werb Z, Rafii S
    Cell, 2002-05-31;109(5):625-37.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  20. Requirement of interleukin 17 receptor signaling for lung CXC chemokine and granulocyte colony-stimulating factor expression, neutrophil recruitment, and host defense.
    Authors: Ye P, Rodriguez FH, Kanaly S, Stocking KL, Schurr J, Schwarzenberger P, Oliver P, Huang W, Zhang P, Zhang J, Shellito JE, Bagby GJ, Nelson S, Charrier K, Peschon JJ, Kolls JK
    J. Exp. Med., 2001-08-20;194(4):519-27.
    Applications: ELISA (Standard)

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Reviews for Recombinant Mouse G-CSF Protein

Average Rating: 4.3 (Based on 3 Reviews)

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