Recombinant Mouse Galectin-2 Protein, CF

Catalog #: 8579-GA Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
8579-GA-050

R&D Systems Recombinant Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Galectin-2 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by the ability of the immobilized protein to support the adhesion of EOMA mouse endothelial cells. The ED₅₀ for this effect is 0.5-2.5 μg/mL. Measured by its ability to agglutinate human red blood cells. Hadari, Y.R. et al. (2000) J. Cell Sci. 113:2385. The ED₅₀ for this effect is 2.5-12.5 μg/mL.

Source

Human embryonic kidney cell, HEK293-derived mouse Galectin-2 protein
Ser2-Glu130

Accession #

AAH27504

N-terminal Sequence
Analysis

Ser2

Predicted Molecular Mass

15 kDa

SDS-PAGE

12-14 kDa, reducing conditions

Product Datasheets

8579-GA

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8579-GA

Formulation	Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl, EDTA, DTT and Trehalose.
Reconstitution	Reconstitute at 400 μg/mL in water.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Galectin-2

Galectin-2 is an approximately 14 kDa, homodimeric carbohydrate-binding protein of the S-type lectin family (1, 2). Like other prototype Galectins, Galectin-2 consists of a single carbohydrate-recognition domain (CRD) (3). Mouse Galectin-2 shares 86% and 66% amino acid sequence identity with rat and human Galectin-2, respectively. Galectin-2 is primarily expressed in the digestive tract and skin of mice (4-6). It can be S-nitrosylated with S-nitrosoglutathione in mouse gastric mucosa (7).
S-nitrosylation prevents Galectin-2 inactivation by hydrogen peroxide, suggesting that this modification protects Galectin-2 function against oxidative stress (8). Galectin-2 appears to have a role in immune responses. Galectin-2 mRNA and protein levels are down-regulated following infection with H. felis (9). Additionally, Galectin-2 treatment has been shown to reduce MHC class I-restricted immune responses and ameliorate acute and chronic experimental colitis in mice by inducing apoptosis of T cells (6, 10). Galectin-2 may also play a role in cancer since circulating levels of human Galectin-2 are elevated in colon and breast cancer patients (11).

References

Hokama, A. et al. (2008) World J. Gastroenterol. 14:5133.
Barondes, S.H. et al. (1994) Cell 76:597.
Hirabayashi, J. and K. Kasai (1993) Glycobiology 3:297.
Nio, J. et al. (2005) J. Histochem. Cytochem. 53:1323.
Nio-Kobayashi, J. et al. (2009) J. Histochem. Cytochem. 57:41.
Loser, K. et al. (2009) J. Immunol. 182:5419.
Ohtake, K. et al. (2009) J. Proteomics 72:750.
Tamura, M. et al. (2015) Biochem. Biophys. Res. Commun. 457:712.
Takaishi, S. and T.C. Wang (2007) Cancer Sci. 98:284.
Paclik, D. et al. (2008) J. Mol. Med. 86:1395.
Chen, C. et al. (2014) Br. J. Cancer 110:741.

Entrez Gene IDs

3957 (Human); 107753 (Mouse); 171134 (Rat)

Alternate Names

Beta-galactoside-binding lectin L-14-II; GAL2; Gal-2; galectin 2; Galectin2; Galectin-2; HL14gal-2; Lactose-binding lectin 2; lectin, galactoside-binding, soluble, 2; LGALS2; MGC75071; S-Lac lectin 2