Recombinant Mouse GFR alpha-1 His-tag Protein, CF

Catalog #: 10070-GR Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10070-GR-050

Recombinant Mouse GFR alpha-1 His-tag Protein Binding Activity

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All GFR alpha-1/GDNF R alpha-1 Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse GFR alpha-1 His-tag Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Rat GDNF (Catalog # 512-GF) is coated at 25 ng/mL (100 μL/well), the concentration of Recombinant Mouse GFR alpha ‑1/GDNF R alpha ‑1 that produces 50% optimal binding response is 0.04-0.24 μg/mL.

Source

Mouse myeloma cell line, NS0-derived mouse GFR alpha-1/GDNF R alpha-1 protein
Asp25-Ser430, with a C-terminal 6-His tag

Accession #

P97785

N-terminal Sequence
Analysis

Ala19

Structure / Form

Monomer

Predicted Molecular Mass

47 kDa

SDS-PAGE

57-67 kDa, reducing conditions

Product Datasheets

10070-GR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10070-GR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Rat GDNF (Catalog # 512-GF) is coated at 25 ng/mL (100 µL/well), Recombinant Mouse GFRa-1/GDNF Ra-1 (Catalog # 10070-GR) binds with an ED₅₀ of 0.04-0.24 µg/mL.

SDS-PAGE

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2 μg/lane of Recombinant Mouse GFR alpha ‑1/GDNF R alpha ‑1 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 57 - 67 kDa and 43 - 57 kDa, respectively.

Reconstitution Calculator

Background: GFR alpha-1/GDNF R alpha-1

Mouse GFR alpha -1/GDNF R alpha -1 contains a 24 amino acid (aa) signal peptide, a 406 aa GDNF receptor domain, and a 38 aa propeptide. Like other GPI-linked proteins, mouse GFR alpha -1/GDNF R alpha -1 has a C‑terminal hydrophobic region which is preceded by a three aa residue (ASS) GPI-binding site. Mouse homolog's GDNF receptor domain contains 99% sequence identity with its rat homolog, and 95% sequence identity with its human homolog. The expression of the various GFR alpha s are differentially regulated in the central and peripheral nervous system, suggesting complementary roles for the GFR alpha s in mediating the activities of the GDNF family of neurotrophic factors (1, 2). GFR alpha -1/GDNF R alpha -1 is expressed by neuronal cells, Schwann cells, and injured sciatic nerve where it recruits c-Ret to the lipid rafts (4). It is also over-expressed in a subset of estrogen responsive breast cancers, where it enhances proliferation and cell scattering in response to GDNF (5). GDNF/GFR alpha -1/c-Ret signaling pathway is part of an inflammatory response in breast cancer. TNF alpha and IL-1 beta enhance expression of GDNF in breast cancer tissue (5). Recent studies have discovered that GFR alpha -1/GDNF R alpha -1 expression is non-existent in Alzheimer brain neurons, which leads to no axonal regrowth and apoptosis (6). Addition of exogenous GFR alpha -1/GDNF R alpha -1 to the Alzheimer brain neurons was able to restore survival and axonal regrowth (6).

References

Thompson, J. et al. (1998) Mol. Cell Neurosci. 11:117.
Trupp, M. et al. (1998) Mol. Cell Neurosci. 11:47.
Baloh, R.H. et al. (1998) Proc. Natl. Acad. Sci. U.S.A. 95:5801.
Paratcha, G. et al. (2001) Neuron 29:171.
Esseghir, S. et al. (2007) Cancer Res. 67:11732.
Konishi, Y. et al. (2014) J Neurosci. 34:13127.

Long Name

Glial Cell line-derived Neurotrophic Factor Receptor alpha 1

Entrez Gene IDs

2674 (Human); 14585 (Mouse); 25454 (Rat)

Alternate Names

DKFZp313E1012; DKFZp686J0156; GDNF family receptor alpha 1; GDNF family receptor alpha-1; GDNF R alpha-1; GDNF receptor alpha-1; GDNFRAGFR-ALPHA-1; GDNFR-alpha-1; GDNFRMGC23045; GFR alpha1; GFR alpha-1; GFRA1; GFRa-1; GFR-alpha-1; Glial cell line-derived neurotrophic factor receptor alpha; GPI-linked anchor protein; PI-linked cell-surface accessory protein; RET ligand 1; RET1L; RETL1FLJ10561; TGF-beta related neurotrophic factor receptor 1; TGF-beta-related neurotrophic factor receptor 1; TRNR1FLJ31546