Recombinant Mouse GFR alpha-like Fc Chimera Protein, CF

Catalog #: 9844-GR Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9844-GR-050

Recombinant Mouse GFR alpha-like Fc Chimera Protein Bioactivity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse GFR alpha-like Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse GFR alpha ‑like Fc Chimera is coated at 0.1 μg/mL, 100 μL/well, it binds to Biotinylated Recombinant Mouse GDF-15 with an ED₅₀ of 0.3-1.8 ng/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived mouse GFR alpha-like protein

Mouse GFR alpha-like (Gln20-Gly349) Accession # Q6SJE0-1	IEGRMDP	Mouse IgG_2a (Glu98-Lys330)
N-terminus		C-terminus

Accession #

Q6SJE0-1

N-terminal Sequence
Analysis

No results obtained. Gln20 inferred from enzymatic pyroglutamate treatment revealing Thr21

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

64 kDa

SDS-PAGE

66-85 kDa, reducing conditions

Product Datasheets

9844-GR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9844-GR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	12 months from date of receipt, ≤ -20 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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When Recombinant Mouse GFR alpha ‑like Fc Chimera (Catalog # 9844-GR) is immobilized at 0.1 μg/mL, 100 μL/well, It binds to Biotinylated Recombinant Mouse GDF-15 with an ED₅₀ of 0.3-1.8 ng/mL.

Reconstitution Calculator

Background: GFR alpha-like

GFR alpha-like (GDNF receptor-alpha-like) is a 25 kDa dimer and distant member of the GDNFR family of proteins (1). It is expressed on both fetal and adult hindbrain neurons of the CNS (2), and would appear to function as an anti-apoptotic molecule during neuronal stress. GFR alpha-like is a funcation receptor of GDF15 and implicated in diet-based obesity and insulin resistance through c-Ret signaling (2 - 4). Mature mouse GFR alpha-like is a 374 amino acid (aa) type I transmembrane protein. It contains a 330 aa extracellular region that shows a Gas1 domain (aa 220‑316), and a 23 aa cytoplasmic tail. Over aa 19‑353, mouse GFR alpha-like shares 72% and 71% aa identity with human and rat GFR alpha–like, respectively.

References