Recombinant Mouse Glypican 2 Protein, CF

Catalog #: 2355-GP
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
2355-GP-050

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Citations (3)

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Glypican 2 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized rmGlypican 2 at 4 µg/mL (100 µL/well) can bind rhMidkine with a linear range of 4-200 ng/mL.

Source

Mouse myeloma cell line, NS0-derived mouse Glypican 2 protein
Lys27-Arg358 & Ala359-Leu554, with a C-terminal 6-His tag

Accession #

Q8BKV1

N-terminal Sequence
Analysis

Lys27 & Ala359

Predicted Molecular Mass

58.9 kDa

SDS-PAGE

60 kDa, non-reducing conditions

Product Datasheets

2355-GP

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2355-GP

Formulation	Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Glypican 2

The glypicans (GPC) constitute a family of heparan sulfate proteoglycans that are attached to the cell surface by a glycosylphosphatidylinositol (GPI) anchor. Six members of this family have been identified in mammals (GPC1 - GPC6). All glypican core proteins contain an N-terminal signal peptide, a large globular cysteine-rich domain (CRD) with 14 invariant cysteine residues, a stalk-like region containing the heparan sulfate attachment sites, and a C-terminal GPI attachment site. Based on the degree of their amino acid sequence similarity, two subfamilies of glypicans have been defined. One subfamily (sharing from 35 - 63% sequence homology) includes GPC1, 2, 4, and 6, while the second subfamily (sharing 54% sequence identity) includes GPC3 and 5. Proteins between the two subfamilies also share 17 - 25% sequence similarity (1 - 4). Glypicans are widely expressed in adult and fetal tissues. During embryonic development, the expression level of the various glypicans changes in a stage and tissue specific manner. GPC2, also known as cerebroglycan, is primarily expressed in developing neuronal tissues including the brain, spinal cord, dorsal root ganglia, and cranial nerves. It is found on the tracts of actively growing axons (5). Cell surface GPC2 binds midkine, indicating midkine-GPC2 interaction may participate in neuronal cell migration and neurite outgrowth (6).

References

Filmus, J. and S.B. Selleck (2001) J. Clin. Invest. 108:497.
De Cat, B. and G. David (2001) Semin. Cell Dev. Biol. 12:117.
Song, H. and J. Filmus (2002) Biochem. Biophys. Acta 1573:241.
Veugelers, M. et al. (1999) J. Biol. Chem. 274:26968.
Ivins, J.K. et al. (1997) Dev. Biol. 184:320.
Kurosawa, N. et al. (2001) Glycoconj. J. 18:499.

Entrez Gene IDs

221914 (Human); 71951 (Mouse)

Alternate Names

2410016G05Rik; Cerebroglycan; Glypican 2; GPC2

Related Research Areas

Citations for Recombinant Mouse Glypican 2 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Identification of the growth cone as a probe and driver of neuronal migration in the injured brain
Authors: Nakajima, C;Sawada, M;Umeda, E;Takagi, Y;Nakashima, N;Kuboyama, K;Kaneko, N;Yamamoto, S;Nakamura, H;Shimada, N;Nakamura, K;Matsuno, K;Uesugi, S;Vep?ek, NA;Küllmer, F;Nasufovi?, V;Uchiyama, H;Nakada, M;Otsuka, Y;Ito, Y;Herranz-Pérez, V;García-Verdugo, JM;Ohno, N;Arndt, HD;Trauner, D;Tabata, Y;Igarashi, M;Sawamoto, K;
Nature communications
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
Glypican-2 levels in cerebrospinal fluid predict the status of adult hippocampal neurogenesis
Authors: S Lugert, T Kremer, R Jagasia, A Herrmann, S Aigner, C Giachino, I Mendez-Dav, AM Gardier, JP Carralot, H Meisterman, A Augustin, MD Saxe, J Lamerz, G Duran-Pach, A Ducret, V Taylor, DJ David, C Czech
Sci Rep, 2017-04-25;7(0):46543.
Species: Mouse
Sample Types: Antibody
Applications: ELISA (Standard)
Protein Tyrosine Phosphatase PTPRS Is an Inhibitory Receptor on Human and Murine Plasmacytoid Dendritic Cells.
Authors: Bunin A, Sisirak V, Ghosh H, Grajkowska L, Hou Z, Miron M, Yang C, Ceribelli M, Uetani N, Chaperot L, Plumas J, Hendriks W, Tremblay M, Hacker H, Staudt L, Green P, Bhagat G, Reizis B
Immunity, 2015-07-28;43(2):277-88.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay