Recombinant Mouse Guanylyl Cyclase C/GUCY2C Fc Protein, CF

Catalog #: 10574-GC Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10574-GC-050

Recombinant Mouse Guanylyl Cyclase C/GUCY2C Fc Chimera Protein SDS-PAGE

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All Guanylyl Cyclase C/GUCY2C Proteins and Enzymes

Product Details

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Guanylyl Cyclase C/GUCY2C Fc Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Bioassay data are not available.

Source

Human embryonic kidney cell, HEK293-derived mouse Guanylyl Cyclase C/GUCY2C protein

Mouse Guanylyl Cyclase C/GUCY2C (Val20-Gln430) Accession # Q3UWA6.1	IEGRMD	Mouse IgG1 (Pro100-Lys330)
N-terminus		C-terminus

Accession #

Q3UWA6.1

N-terminal Sequence
Analysis

Val20

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

74 kDa

SDS-PAGE

109-124 kDa, under reducing conditions

Product Datasheets

10574-GC

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10574-GC

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

SDS-PAGE

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2 μg/lane of Recombinant Mouse Guanylyl Cyclase C/GUCY2C Fc Chimera Protein (Catalog # 10574-GC) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 109-124 kDa and 218-248 kDa, respectively.

Reconstitution Calculator

Background: Guanylyl Cyclase C/GUCY2C

Guanylyl Cyclase C (GUCY2C), also known as heat-stable enterotoxin receptor, is a type I transmembrane protein of the guanylate cyclase (GC) family expressed by intestinal epithelial cells from the duodenum to rectum. It was first identified as the intestinal epithelial receptor regulating fluid and electrolyte transport in the secretory diarrhea induced by bacterial enterotoxins (1). There are 7 known members of the GC family (GC-A through GC-G) and they catalyze the conversion of guanosine triphosphate to cyclic guanosine monophosphate (cGMP) and pyrophosphate (2-4). Mature mouse GUCY2C consists of an extracellular domain (ECD) with a ligand binding domain, a transmembrane segment and a cytoplasmic region possessing a kinase and GC catalytic domain. The ECD of mouse GUCY2C shares 70% and 88% amino acid sequence identity with the ECD of human and rat GUCY2C, respectively. Endogenous ligands of GUCY2C include guanylin and uroguanylin (5). GUCY2C in epithelial cells plays an important role in cell dynamics and homeostatic balance of proliferation, metabolism, and differentiation that organizes the guanylyl cyclase C hormone axis. GUCY2C is also expressed in the brain and is implicated in attention deficiency and hyperactive behavior (5-7).

References

Lucas K. et al. (2000) Pharmacol. Rev. 52:375.
Potter, L. R. (2011) Cell Signal. 23:1921.
Arshad, N. et al. (2013) J. Biol. Chem. 288:3907.
Gibbons, A. V. et al. (2013) Cancer Res. 73:22.
Erik, S. et al. (2016) Mol. Pharmacol. 90:199.
Gibbons, A. V. et al. (2013) Cancer Res. 73:22.
Gong, R. et al. (2011) Science 333:1642.

Entrez Gene IDs

2984 (Human); 14917 (Mouse); 25711 (Rat)

Alternate Names

DIAR6; EC 4.6.1; GCC; GC-C; guanylate cyclase 2C (heat stable enterotoxin receptor); Guanylate Cyclase C; Guanylyl Cyclase C; GUC2C; GUC2CEC 4.6.1.2; GUCY2C; heat-stable enterotoxin receptor; hSTAR; Intestinal guanylate cyclase; MUCIL; STA Receptor; StAR; STARSTA receptor