Home / ICAM-2/CD102 / Recombinant Mouse ICAM-2/CD102 Fc Chimera Protein, CF

Recombinant Mouse ICAM-2/CD102 Fc Chimera Protein, CF

Catalog #: 774-M2
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
774-M2-050
774-M2-200
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Citations (3)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse ICAM-2/CD102 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. Recombinant Mouse ICAM‑2/CD102 Fc Chimera immobilized at 12.5 µg/mL (100 µL/well) will induce >65% cell adhesion.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived mouse ICAM-2/CD102 protein
Mouse ICAM-2
(Ser20-Gln222)
Accession # P35330		 DIEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
P35330
N-terminal Sequence
Analysis
Ser20
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
49.5 kDa (monomer)
SDS-PAGE
75-80 kDa, reducing conditions

Product Datasheets

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774-M2

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

774-M2

Formulation Lyophilized from a 0.2 μm filtered solution in Tris, Citric Acid and NaCl.
Reconstitution Reconstitute at 0.4 mg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ICAM-2/CD102

Intercellular Adhesion Molecule-2 (ICAM-2, CD102), a member of the immunoglobulin superfamily, binds the leukocyte integrins LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). ICAM-2 is constitutively expressed at high levels on vascular endothelial cells and lymphohematopoietic cells. ICAM-2 mediated adhesion has been shown to provide a co-stimulatory signal for T cell aggregation, NK cytotoxicity and NK cell migration. The mouse and human ICAM-2 proteins share 60% amino acid identity. Human LFA-1 binds equally well to human or mouse ICAM-2.

References
  1. Somersalo, K. et al. (1995) J. Biol. Chem. 270:8629.
  2. Hayflick, J. et al. (1998) Immunologic Res. 17:313.
  3. Xu, H. (1992) J. Immunol. 149:2650.
Long Name
Intercellular Adhesion Molecule 2
Entrez Gene IDs
3384 (Human); 15896 (Mouse)
Alternate Names
CD102 antigen; CD102; ICAM2; ICAM-2; intercellular adhesion molecule 2

Citations for Recombinant Mouse ICAM-2/CD102 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Neuronal ICAM-5 Inhibits Microglia Adhesion and Phagocytosis and Promotes an Anti-inflammatory Response in LPS Stimulated Microglia
    Authors: S Paetau, T Rolova, L Ning, CG Gahmberg
    Front Mol Neurosci, 2017-12-22;10(0):431.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. ICAM-2 facilitates luminal interactions between neutrophils and endothelial cells in vivo.
    Authors: Halai, Krishma, Whiteford, James, Ma, Bin, Nourshargh, Sussan, Woodfin, Abigail
    J Cell Sci, 2013-12-06;127(0):620-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Vitronectin induces phosphorylation of ezrin/radixin/moesin actin-binding proteins through binding to its novel neuronal receptor telencephalin.
    Authors: Furutani Y, Kawasaki M, Matsuno H, Mitsui S, Mori K, Yoshihara Y
    J Biol Chem, 2012-09-27;287(46):39041-9.
    Applications: Surface Plasmon Resonance

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