Recombinant Mouse IFN-alpha 1 Protein, CF

Catalog #: 10148-IF
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10148-IF-010
10148-IF-050

Recombinant Mouse IFN-alpha 1 Protein Bioactivity

2 Images

Product Details

Citations (1)

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse IFN-alpha 1 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured in an anti-viral assay using L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. Vogel, S.N. et al. (1982) Infect. Immunol. 38:681. The ED₅₀for this effect is 1.2-12 pg/mL.

Source

Human embryonic kidney cell, HEK293-derived mouse IFN-alpha 1 protein
Cys24-Lys189

Accession #

P01572

N-terminal Sequence
Analysis

No results obtained. Cys24 inferred from enzymatic pyroglutamate treatment revealing Asp25.

Predicted Molecular Mass

19 kDa

SDS-PAGE

18-22 kDa, under reducing conditions

Product Datasheets

10148-IF

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10148-IF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after opening. 3 months, -20 to -70 °C under sterile conditions after opening.

Scientific Data

Bioactivity

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Recombinant Mouse IFN-alpha 1 (Catalog # 10148-IF) suppresses viral activity on L-929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. The ED₅₀ for this effect is 1.2-12 pg/mL.

SDS-PAGE

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2 μg/lane of Recombinant Mouse IFN-alpha 1 (Catalog # 10148-IF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 18-22 kDa.

Reconstitution Calculator

Background: IFN-alpha 1

The interferons (IFN) are a family of cytokines with potent antiviral, antiproliferative and immunomodulatory properties, and classified based on their binding specificity to cell surface receptors (1). The type I IFN bind to the interferon alpha receptor (IFNAR), which consists of two subunits: IFNAR1 ( alpha -subunit) and IFNAR2 ( beta -subunit). This binding contributes to TNF-alpha induced signaling (2, 3). Both the human and mouse genome code for more than a dozen closely related IFNa subtypes and the varous IFN a share about 80% sequence homology among them (4-5). Interferon-alpha 1 (IFNA1) is a secreted, approximately 19 kDa member of the type I interferon family of molecules (6). Mature mouse IFN-alpha 1 shares 63% and 82% amino acid sequence identity with human and rat IFN-alpha 1, respectively. Low level IFN-alpha is detected under physiological conditions, and the production of IFNs is markedly enhanced during virus infection (7). Although originally discovered by its capability to fight virus replication, IFN-alpha functions as a prototypic tumor suppressor that represses the clinical tumorigenic phenotype in some malignancies (7).

References

Pestka, S. et al. (1987) Annu Rev Biochem. 56:727.
Fung, K.Y. et al. (2013) Science 339:1088.
Matsumiya, T. et al. (2007) J. Immunol. 179:4542.
Schreiber, G. and J. Piehler (2015) Trends Immunol. 36: 139.
van Pesch, V. et al. (2004) J. Virol. 78: 8219.
Wijesundara, D.K. et al. (2014) Front. Immunol. 5:412.
Gutterman J. U. et al. (1994) Proc. Natl. Acad. Sci. U. S. A. 91:1198.

Long Name

Interferon alpha 1

Entrez Gene IDs

3439 (Human); 15962 (Mouse); 690894 (Rat)

Alternate Names

IFL;IFN;IFNA@;IFNA13;IFN-ALPHA;IFN-alphaD;Interferon alpha-1/13;leIF D; IFNA1; IFNalpha 1; IFN-alpha 1

Related Research Areas

Interferons

Citation for Recombinant Mouse IFN-alpha 1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Deconvoluting global cytokine signaling networks in natural killer cells
Authors: GM Wiedemann, EK Santosa, S Grassmann, S Sheppard, JB Le Luduec, NM Adams, C Dang, KC Hsu, JC Sun, CM Lau
Nature Immunology, 2021-04-15;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay