Recombinant Mouse IFN-alpha 2/IFNA2 Protein, CF

Catalog #: 10149-IF
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10149-IF-010
10149-IF-050

Recombinant Mouse IFN-alpha 2/IFNA2 Protein Bioactivity

2 Images

All IFN-alpha 2/IFNA2 Proteins and Enzymes

Product Details

Citations (2)

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse IFN-alpha 2/IFNA2 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured in an anti-viral assay using L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. Vogel, S.N. et al. (1982) Infect. Immunol. 38:681. The ED₅₀ for this effect is 20-200 pg/mL.

Source

Human embryonic kidney cell, HEK293-derived mouse IFN-alpha 2/IFNA2 protein
Cys24-Glu190

Accession #

P01573

N-terminal Sequence
Analysis

Cys24

Predicted Molecular Mass

19.3 kDa

SDS-PAGE

18-22 kDa, under reducing conditions

Product Datasheets

10149-IF

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10149-IF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:

Scientific Data

Bioactivity

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Recombinant Mouse IFN-alpha 2/IFNA2 (Catalog # 10149-IF) suppresses viral activity on L-929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. The ED₅₀ for this effect is 20-200 pg/mL.

SDS-PAGE

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2 μg/lane of Recombinant Mouse IFN-alpha 2/IFNA2 (Catalog # 10149-IF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 18-22 kDa.

Reconstitution Calculator

Background: IFN-alpha 2/IFNA2

Interferon-alpha 2 (IFN alpha -2) is one of 14 subtypes within the IFN-alpha class of Type I Interferons (1). The members of the IFN-alpha class, also known as alpha leukocyte interferons, encompass a group of distinct but closely related proteins which share approximately 80% amino acid (aa) sequence identity and have a similar globular structure composed of five alpha-helices (1, 3, 4). IFN-alpha class members signal through a common cell surface receptor complex composed of IFN-alpha R2 and IFN-alpha R1 subunits (3). As the first highly active IFN to be cloned and produced, IFN alpha -2 has become the prototypic IFN for academic and pharmaceutical research (2). The mature extracellular domain (ECD) of mouse IFN alpha -2 shares 60% and 83% aa sequence identity with human and rat, respectively. Murine IFN-alpha 2 can eliminate cardiac viral load and protect cardiomyocytes from injury in animals infected with coxsackievirus B3 (CVB3) (5). IFN alpha -2 derived mutants with reduced IFNR2 binding inhibited HIV replication and mutants with more IFNAR1 binding potentiated antiviral activity (6).

References

Pestka, S. (2007) J Biol Chem. 282:20047.
Paul, F. et al. (2015) Gene. 567(2):132.
Oritani, K. et al. (2001). Cytokine & Growth Factor Reviews, 12:337.
Pesch, V. et al. (2004). Journal of Virology, 78:8219.
Wang, Y.X. et al. (2007) Am J Physiol Heart Circ Physiol. 293:H69.
Schlaepfer, E. et al. (2019) Am Soc for Microbiology 4:e00637.

Long Name

Interferon alpha 2

Entrez Gene IDs

3440 (Human); 15965 (Mouse); 298213 (Rat)

Alternate Names

alpha-2a interferon; Ifa2; IFNA; IFNA2; IFNA2a; IFNA2b; IFNA2c; IFNAA; IFNalpha 2; IFN-alpha 2; IFN-alpha-2; IFN-alphaA; INFA2; interferon alpha 2b; interferon alpha A; interferon alpha-2; Interferon alpha-A; interferon, alpha 2; LeIF A; MGC125764; MGC125765

Related Research Areas

Interferons

Citations for Recombinant Mouse IFN-alpha 2/IFNA2 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Toxicity and efficacy of type I interferons on the ocular surface: in vitro, animal, and clinical studies
Authors: Yun, YI;Ko, JH;Ryu, JS;Kim, S;Jeon, HS;Kim, N;Kim, MK;Oh, JY;
The ocular surface
Species: Mouse
Sample Types: In Vivo
Applications: In vivo assay
Triplication of the interferon receptor locus contributes to hallmarks of Down syndrome in a mouse model
Authors: Waugh, KA;Minter, R;Baxter, J;Chi, C;Galbraith, MD;Tuttle, KD;Eduthan, NP;Kinning, KT;Andrysik, Z;Araya, P;Dougherty, H;Dunn, LN;Ludwig, M;Schade, KA;Tracy, D;Smith, KP;Granrath, RE;Busquet, N;Khanal, S;Anderson, RD;Cox, LL;Estrada, BE;Rachubinski, AL;Lyford, HR;Britton, EC;Fantauzzo, KA;Orlicky, DJ;Matsuda, JL;Song, K;Cox, TC;Sullivan, KD;Espinosa, JM;
Nature genetics
Species: Transgenic Mouse
Sample Types: Whole Cells
Applications: Bioassay