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Recombinant Mouse IGFBP-L1 Protein, CF

Catalog #: 4130-BL
2 Citations Datasheet / COA / SDS
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4130-BL-050
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Citations (2)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse IGFBP-L1 Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit proliferation of HeLa human cervical epithelial carcinoma cells. Cai, Z. et al. (2005) BBRC 331:261. The ED50 for this effect is 2-8 µg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse IGFBP-L1 protein
Leu18-Leu270, with a C-terminal 10-His tag
Accession #
Q80W15
N-terminal Sequence
Analysis
Leu18
Predicted Molecular Mass
28.2 kDa
SDS-PAGE
36 kDa, reducing conditions

Product Datasheets

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4130-BL

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

4130-BL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IGFBP-L1

Insulin-like Growth Factor Binding Protein-Like 1, also known as IGFBP-rP4, is a 38 kDa secreted member of the IGFBP superfamily of proteins. IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins (1). IGFBP-L1 contains an N-terminal IGFBP motif, a Kazal-type serine protease inhibitor region, and a C-terminal Ig-like domain (2, 3). Mature mouse IGFBP-L1 shares 76% and 96% amino acid (aa) sequence identity with human and rat IGFBP-L1, respectively. It shares 38% - 41% aa sequence identity with mouse IGFBP-7 and IGFBP-rp10 and 20% - 24% aa sequence identity with mouse IGFBP-1, -2, -3, -4, -5, and -6. IGFBP-L1 is most highly expressed in testis and brain, while lower levels are more broadly expressed (3). IGFBP-L1 is expressed in regionally and temporally distinct patterns during forebrain development (4). IGFBP-L1 inhibits cell proliferation in vitro and is downregulated in many colon and lung tumors (3). Transcriptional downregulation by promoter methylation is predictive of breast cancer aggressiveness (5).

References
  1. Firth, S.M. and R.C. Baxter (2002) Endocr. Rev. 23:824.
  2. Accession # Q80W15.
  3. Cai, Z. et al. (2005) Biochem. Biophys. Res. Commun. 331:261.
  4. Gonda, Y. et al. (2007) Gene Expr. Patterns 7:431.
  5. Smith, P. et al. (2007) Clin. Cancer Res. 13:4061.
Long Name
Insulin-like Growth Factor Binding Protein-like 1
Entrez Gene IDs
347252 (Human); 75426 (Mouse)
Alternate Names
bA113O24.1; IGFBPL1 insulin-like growth factor binding protein-like 1; IGFBPL1; IGFBP-L1; IGFBP-RP4

Citations for Recombinant Mouse IGFBP-L1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. IGFBPL1 is a master driver of microglia homeostasis and resolution of neuroinflammation in glaucoma and brain tauopathy
    Authors: Pan, L;Cho, KS;Wei, X;Xu, F;Lennikov, A;Hu, G;Tang, J;Guo, S;Chen, J;Kriukov, E;Kyle, R;Elzaridi, F;Jiang, S;Dromel, PA;Young, M;Baranov, P;Do, CW;Williams, RW;Chen, J;Lu, L;Chen, DF;
    Cell reports
    Applications: Cell Culture
  2. Neural precursor cells tune striatal connectivity through the release of IGFBPL1
    Authors: E Butti, S Cattaneo, M Bacigalupp, M Cambiaghi, GM Scotti, E Brambilla, F Ruffini, G Sferruzza, M Ripamonti, F Simeoni, L Cacciaguer, A Zanghì, A Quattrini, R Fesce, P Panina-Bor, F Giannese, D Cittaro, T Kuhlmann, P D'Adamo, MA Rocca, S Taverna, G Martino
    Nature Communications, 2022-12-08;13(1):7579.
    Species: Mouse
    Sample Types:
    Applications: ELISA Standard

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