Recombinant Mouse IgG2A Fc Protein, CF Summary
Product Specifications
Glu98-Lys330, with an N-terminal Met-Asp-Pro
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
4460-MG
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution | It is recommended that sterile PBS be added to the vial to prepare a working stock solution of no less than 100 μg/mL. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: IgG2A
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Citations for Recombinant Mouse IgG2A Fc Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Sema6D forward signaling impairs T cell activation and proliferation in head and neck cancer
Authors: Hirai, T;Naito, Y;Koyama, S;Nakanishi, Y;Masuhiro, K;Izumi, M;Kuge, T;Naito, M;Mizuno, Y;Yamaguchi, Y;Kang, S;Yaga, M;Futami, Y;Nojima, S;Nishide, M;Morita, T;Kato, Y;Tsuda, T;Takemoto, N;Kinugasa-Katayama, Y;Aoshi, T;Villa, JK;Yamashita, K;Enokida, T;Hoshi, Y;Matsuura, K;Tahara, M;Takamatsu, H;Takeda, Y;Inohara, H;Kumanogoh, A;
JCI insight
Species: Mouse
Sample Types: cell culture
Applications: Stimulation -
GITR/GITRL reverse signalling modulates the proliferation of hepatic progenitor cells by recruiting ANXA2 to phosphorylate ERK1/2 and Akt
Authors: Y He, Y Pei, K Liu, L Liu, Y Tian, H Li, M Cong, T Liu, H Ma, H You, J Jia, D Zhang, P Wang
Cell Death & Disease, 2022-04-04;13(4):297.
Species: Rat
Sample Types: Whole Cells
Applications: Cell Culture -
Invariant NKT Cell Activation Is Potentiated by Homotypic trans-Ly108 Interactions
Authors: Y Baglaenko, M Cruz Tleug, E Gracey, N Talaei, KP Manion, NH Chang, DM Ferri, T Mallevaey, JE Wither
J. Immunol., 2017-04-03;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Differential but competitive binding of Nogo protein and class i major histocompatibility complex (MHCI) to the PIR-B ectodomain provides an inhibition of cells.
Authors: Matsushita H, Endo S, Kobayashi E, Sakamoto Y, Kobayashi K, Kitaguchi K, Kuroki K, Soderhall A, Maenaka K, Nakamura A, Strittmatter SM, Takai T
J. Biol. Chem., 2011-06-02;286(29):25739-47.
Species: Mouse
Sample Types: Whole Cells
Applications: Control
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