Recombinant Mouse IGSF2/CD101 Fc Chimera Protein, CF

Catalog #: 3368-CD Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
3368-CD-050

Recombinant Mouse IGSF2/CD101 Fc Chimera Protein Bioactivity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse IGSF2/CD101 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells. The ED₅₀ for this effect is 1.4-8.4 μg/mL.

Source

Mouse myeloma cell line, NS0-derived mouse IGSF2/CD101 protein

Mouse CD101 (Gln21-Phe974) Accession # A8E0Y8	IEGRMDP	Mouse IgG_2a (Glu98-Lys330)
N-terminus		C-terminus

Accession #

A8E0Y8

N-terminal Sequence
Analysis

No results obtained. Gln21 inferred from enzymatic pyroglutamate treatment revealing Arg22.

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

133 kDa

SDS-PAGE

121-150 kDa, reducing conditions

Product Datasheets

3368-CD

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3368-CD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 400 μg/mL in PBS.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant Mouse IGSF2/CD101 Fc Chimera (Catalog # 3368-CD) inhibits anti-CD3 antibody induced human T cell proliferation. The ED₅₀ for this effect is 1.4-8.4 μg/mL.

SDS-PAGE

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2 μg/lane of Recombinant Mouse IGSF2/CD101 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 121-150 kDa and 240-280 kDa, respectively.

Reconstitution Calculator

Background: IGSF2/CD101

CD101, also known as Immunoglobulin Superfamily member 2 (IGSF2) or cell surface glycoprotein V7 (V7), is a 130-145 kDa type 1 transmembrane glycoprotein within the very large Ig superfamily (1). Ig superfamily members participate in diverse functions including cell-cell recognition and adhesion, soluble molecules and specific antigen recognition, and are grouped based on the presence of a characteristic Ig domain fold. Mature mouse CD101 consists of a large extracellular domain (ECD) containing a Glu-Trp-Ile (EWI) motif and 7 Ig-like V-type domains, a single transmembrane domain, and a short cytoplasmic domain (1, 2). The mouse CD101 ECD shares 71% and 87% amino acid sequence identity with human and rat CD101 ECD, respectively. The exact biological functions, interacting ligands and mode of signal transduction for CD101 currently remain unclear. CD101 expression has been found on dermal dendritic cells (DCs), granulocytes, monocytes, activated T cells, and CD4⁺ CD25⁺ FoxP3⁺ T regulatory cells (Tregs) (2-5). CD101 expression has been linked to immune suppression by Tregs in humans (6), with ligation of CD101 on DCs inducing IL-10 expression (2) and ligation on activated T cells blocking IL-2 expression (7). Both of these effects down-regulate T cell activity. Notably, in mice, CD101 expression on CD62L⁺⁺ Tregs identifies a population of cells that have potent suppressor activity (5). In humans, reduced CD101 expression on mucosal CD8⁺ T cells has been linked to increased tissue inflammation in both intestinal (8) and pulmonary mucosa (9). In both mouse and human, mutations in CD101 have been associated with increased susceptibility for diabetes (10, 11). CD101 expression may act as a potential biomarker for CD4⁺ CD56⁺ blastic tumors (12) and inflammatory bowel disease activity (13), and missense variants of its gene have been associated with increased risk of HIV-1 acquisition (14).

References

Stipp, C. et al. (2001) J. Biol. Chem. 276:40545.
Bouloc, A. et al. (2000) Eur. J. Immunol. 30:3132.
Rivas, A. et al. (1995) J. Immunol. 154:4423.
Ruegg, C. et al. (1995) J. Immunol. 154:4434-CD.
Fernandez, I. et al. (2007) J. Immunol. 179:2808.
Hua, J. et al. (2015) J. Immunol. 195:3642.
Soares, L. et al. (1998) J. Immunol. 161:209.
Brimnes, J. et al. (2005) J. Immunol. 174:5814.
Kumar, B. et al. (2017) Cell Rep. 20:2921.
Rainbow, D. et al. (2011) J Immunol. 187:325.
Okuno, M. et al. (2017) J Diabetes Investig. 8:286.
Meyer, N. et al. (2005) J. Invest. Dermatol. 124:668.
Schey, R. et al. (2016) Mucosal Immunol. 9:1205.
Mackelprang, R. et al. (2017) PLoS Pathog. 13:e1006703.

Long Name

Immunoglobulin Superfamily, Member 2

Entrez Gene IDs

9398 (Human); 630146 (Mouse)

Alternate Names

CD101 antigen; CD101 molecule; CD101; Cell surface glycoprotein V7; EWI101; EWI-101; Glu-Trp-Ile EWI motif-containing protein 101; IGSF2; Leukocyte Surface Protein; member 2; V7