Recombinant Mouse IL-10 R alpha Protein, CF

Name: Recombinant Mouse IL-10 R alpha Protein, CF
Brand: R&D Systems
SKU: 474-MR/CF

Catalog #: 474-MR/CF Datasheet / COA / SDS

Newer Version Available: 7704-MR/CF

NEW

A New and Improved rm IL-10 Ra is Now Available! It has a lower endotoxin specification!

Discontinued Product

474-MR/CF has been discontinued and is replaced by 7704-MR/CF.

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All IL-10 R alpha Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse IL-10 R alpha Protein, CF Summary

Product Specifications

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit IL-10-dependent proliferation of MC/9‑2 mouse mast cells. Thompson-Snipes, L. et al. (1991) J. Exp. Med. 173:507. Approximately 1-3 µg/mL of recombinant mouse IL-10 sR alpha will inhibit 50% of the biological response due to 1 ng/mL of recombinant mouse
IL-10.

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse IL-10 R alpha protein
Thr24-Asn238 (Lys188Arg and Glu222Gly)

Accession #

Q61727

N-terminal Sequence
Analysis

Thr24

Predicted Molecular Mass

24 kDa

SDS-PAGE

31 kDa, reducing conditions

Product Datasheets

474-MR/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

474-MR/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 200 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: IL-10 R alpha

Interleukin-10 Receptor alpha (IL-10 R alpha ), also known as IL-10 R1, is a 90-110 kDa transmembrane glycoprotein member of the class II cytokine receptor family (1). IL-10 R alpha is required for mediating the effects of IL-10, a critical molecule in the control of microbial infections, allergic and autoimmune inflammation, and cancer
(2-5). Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells (6). IL-10 R alpha is the ligand specific subunit of the IL-10 receptor complex. Noncovalent dimers of IL-10 bind to IL‑10 R alpha, resulting in the recruitment of IL-10 R beta (6-8). IL-10 R beta is a ubiquitously expressed transmembrane protein that does not bind IL-10 by itself but is required for signal transduction and in vivo IL-10 responsiveness (7, 9). IL-10 R beta also associates with IL-20 R alpha, IL-22 R alpha, or
IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29 (1). Immunosuppressive signal transduction through the IL-10 receptor complex can be inhibited by activation of TLR2, 4, or 9, enabling strengthened immune responses during infection (10). Some polymorphisms of human IL-10 R alpha may limit viral immune evasion by retaining full responsiveness to human IL-10 but responding weakly to the cytomegalovirus homolog of IL-10 (11). Mature mouse IL-10 R alpha consists of a 225 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 313 aa cytoplasmic domain (12). Within the ECD, mouse
IL-10 R alpha shares 59% and 78% aa sequence identity with human and rat IL-10 R alpha, respectively.

References

Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
Manzanillo, P. et al. (2015) Trends Immunol. 36:471.
Sziksz, E. et al. (2015) Mediators Inflamm. 2015:764641.
Mannino, M.H. et al. (2015) Cancer Lett. 367:103.
Fitzgerald, D.C. et al. (2007) Nat. Immunol. 8:1372.
Tan, J.C. et al. (1993) J. Biol. Chem. 268:21053.
Kotenko, S.V. et al. (1997) EMBO J. 16:5894.
Tan, J.C. et al. (1995) J. Biol. Chem. 270:12906.
Spencer, S.D. et al. (1998) J. Exp. Med. 187:571.
Fernandez, S. et al. (2004) J. Immunol. 172:2613.
Gruber, S.G. et al. (2008) Eur. J. Immunol. 38:3365.
Ho, A.S.-Y. et al. (1993) Proc. Natl. Acad. Sci. 90:11267.

Long Name

Interleukin 10 Receptor alpha

Entrez Gene IDs

3587 (Human); 16154 (Mouse); 102127536 (Cynomolgus Monkey)

Alternate Names

CD210 antigen; CDw210a; HIL-10R; IBD28; IL-10 R alpha; IL-10 receptor subunit alpha; IL10R alpha; IL-10R subunit 1; IL-10R1; IL10RA; IL-10Ra; IL10RIL-10R subunit alpha; interleukin 10 receptor, alpha; interleukin-10 receptor alpha chain; Interleukin-10 receptor subunit 1; interleukin-10 receptor subunit alpha