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Recombinant Mouse IL-10 R alpha Protein

Catalog #: 474-MR Datasheet / COA / SDS
Newer Version Available: 7704-MR
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A New and Improved rmIL-10 Ra is Now Available! It has a lower endotoxin specification!

Discontinued Product

474-MR has been discontinued and is replaced by 7704-MR.

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Recombinant Mouse IL-10 R alpha Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit IL-10-dependent proliferation of MC/9‑2 mouse mast cells. Thompson-Snipes, L. et al. (1991) J. Exp. Med. 173:507. Approximately 1-3 µg/mL of recombinant mouse IL-10 sR alpha will inhibit 50% of the biological response due to 1 ng/mL of recombinant mouse
IL-10.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse IL-10 R alpha protein
Thr24-Asn238 (Lys188Arg and Glu222Gly)
Accession #
Q61727
N-terminal Sequence
Analysis
Thr24
Predicted Molecular Mass
24 kDa
SDS-PAGE
31 kDa, reducing conditions

Product Datasheets

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474-MR (with carrier)

Product Datasheet
COA
SDS

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474-MR/CF (carrier free)

Discontinued Product

474-MR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-10 R alpha

Interleukin-10 Receptor alpha (IL-10 R alpha ), also known as IL-10 R1, is a 90-110 kDa transmembrane glycoprotein member of the class II cytokine receptor family (1). IL-10 R alpha is required for mediating the effects of IL-10, a critical molecule in the control of microbial infections, allergic and autoimmune inflammation, and cancer
(2-5). Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells (6). IL-10 R alpha is the ligand specific subunit of the IL-10 receptor complex. Noncovalent dimers of IL-10 bind to IL‑10 R alpha, resulting in the recruitment of IL-10 R beta (6-8). IL-10 R beta is a ubiquitously expressed transmembrane protein that does not bind IL-10 by itself but is required for signal transduction and in vivo IL-10 responsiveness (7, 9). IL-10 R beta also associates with IL-20 R alpha, IL-22 R alpha, or
IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29 (1). Immunosuppressive signal transduction through the IL-10 receptor complex can be inhibited by activation of TLR2, 4, or 9, enabling strengthened immune responses during infection (10). Some polymorphisms of human IL-10 R alpha may limit viral immune evasion by retaining full responsiveness to human IL-10 but responding weakly to the cytomegalovirus homolog of IL-10 (11). Mature mouse IL-10 R alpha consists of a 225 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 313 aa cytoplasmic domain (12). Within the ECD, mouse
IL-10 R alpha shares 59% and 78% aa sequence identity with human and rat IL-10 R alpha, respectively.

References
  1. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
  2. Manzanillo, P. et al. (2015) Trends Immunol. 36:471.
  3. Sziksz, E. et al. (2015) Mediators Inflamm. 2015:764641.
  4. Mannino, M.H. et al. (2015) Cancer Lett. 367:103.
  5. Fitzgerald, D.C. et al. (2007) Nat. Immunol. 8:1372.
  6. Tan, J.C. et al. (1993) J. Biol. Chem. 268:21053.
  7. Kotenko, S.V. et al. (1997) EMBO J. 16:5894.
  8. Tan, J.C. et al. (1995) J. Biol. Chem. 270:12906.
  9. Spencer, S.D. et al. (1998) J. Exp. Med. 187:571.
  10. Fernandez, S. et al. (2004) J. Immunol. 172:2613.
  11. Gruber, S.G. et al. (2008) Eur. J. Immunol. 38:3365.
  12. Ho, A.S.-Y. et al. (1993) Proc. Natl. Acad. Sci. 90:11267.
Long Name
Interleukin 10 Receptor alpha
Entrez Gene IDs
3587 (Human); 16154 (Mouse); 102127536 (Cynomolgus Monkey)
Alternate Names
CD210 antigen; CDw210a; HIL-10R; IBD28; IL-10 R alpha; IL-10 receptor subunit alpha; IL10R alpha; IL-10R subunit 1; IL-10R1; IL10RA; IL-10Ra; IL10RIL-10R subunit alpha; interleukin 10 receptor, alpha; interleukin-10 receptor alpha chain; Interleukin-10 receptor subunit 1; interleukin-10 receptor subunit alpha

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