Recombinant Mouse IL-12 R beta 1 Protein, CF

Catalog #: 1998-B1 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
1998-B1-050

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse IL-12 R beta 1 Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized rmIL-12 R beta 1 at 10 µg/mL (100 µL/well) can bind rmIL-12 with a linear range of 0.3-20 µg/mL.

Source

Mouse myeloma cell line, NS0-derived mouse IL-12 R beta 1 protein
Gln20-Glu561, with a C-terminal 6-His tag

Accession #

Q60837

N-terminal Sequence
Analysis

No results obtained: Gln20 predicted

Predicted Molecular Mass

61.3 kDa

SDS-PAGE

95-110 kDa, reducing conditions

Product Datasheets

1998-B1

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1998-B1

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: IL-12 R beta 1

IL-12 R beta 1 is a 100 kDa type I transmembrane protein that belongs to the gp130/G-CSF R family of cytokine receptors. IL-12 R beta 1 is a common subunit of both the IL-12 and IL-23 receptor complexes which play distinct but related roles in T cell mediated inflammatory reactions (1, 2). Mature mouse IL-12 R beta 1 contains a 546 amino acid (aa) extracellular domain (ECD) with five fibronectin type III repeats, and a 147 aa cytoplasmic domain (3). Within the ECD, mouse IL-12 R beta 1 shares 85% and 52% aa sequence identity with rat and human IL-12 R beta 1, respectively. It shares 16% - 21% aa sequence identity with the ECDs of mouse gp130, LIF R, G-CSF R, and IL-23 R. IL-12 and IL-23 are disulfide linked heterodimeric cytokines that share a common p40 subunit (1, 2). IL-12 R beta 1 interacts with p40 at low affinity but does not transmit signals (3). Increased ligand binding affinity and signaling capacity are gained by association of IL-12 R beta 1 with either IL-12 R beta 2 or IL-23 R (4 - 6). IL-12 R beta 2 and IL-23 R are the signal transducing components of these receptor complexes (4, 7). IL-12 R beta 1 is expressed on activated T cells, NK cells, B cells, macrophages, and microglia (8 - 10). IL-12 induced signaling promotes the development of naïve T cells into IFN-beta producing Th1 cells (11). IL-23 contributes to chronic inflammation by inducing the production of IL-17 by memory T cells (12). Naturally occurring homodimers of p40 can function as antagonists of IL-12 and IL-23 and can also induce macrophage chemotaxis in the absence of IL-12 R beta 2 (13, 14).

References

Becker, C. et al. (2005) Inflamm. Bowel Dis. 11:755.
Hunter, C.A. (2005) Nat. Rev. Immunol. 5:521.
Chua, A.O. et al. (1995) J. Immunol. 155:4286.
Parham, C. et al. (2002) J. Immunol. 168:5699.
Wu, C. et al. (1997) J. Immunol. 159:1658.
Zou, J. et al. (1997) J. Biol. Chem. 272:6073.
Presky, D.H. et al. (1996) Proc. Natl. Acad. Sci. 93:14002.
Wu, C. et al. (1997) Eur. J. Immunol. 27:147.
Airoldi, I. et al. (2000) J. Immunol. 165:6880.
Li, J. et al. (2003) J. Neurol. Sci. 215:95.
Schmitt, E. et al. (1994) Eur. J. Immunol. 24:793.
Yen, D. et al. (2006) J. Clin. Invest. 116:1310.
Shimozato, O. et al. (2006) Immunology 117:22.
Russell, T.D. et al. (2003) J. Immunol. 171:6866.

Long Name

Interleukin 12 Receptor beta 1

Entrez Gene IDs

3594 (Human); 16161 (Mouse)

Alternate Names

CD212 antigen; CD212; IL-12 R beta 1; IL-12 receptor beta component; IL-12 receptor subunit beta-1; IL12R beta 1; IL-12R subunit beta-1; IL12R; IL12RB; IL12RB1; IL-12Rb1; IL-12R-BETA1; IL-12R-beta-1; interleukin 12 receptor, beta 1; interleukin-12 receptor beta-1 chain; interleukin-12 receptor subunit beta-1; MGC34454