Recombinant Mouse IL-13 R alpha 1 Fc Chimera Protein

Catalog #: 491-IR Datasheet / COA / SDS

Carrier Free

Catalog #	Availability	Size / Price	Qty
491-IR-200/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
491-IR-200

R&D Systems Recombinant Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse IL-13 R alpha 1 Fc Chimera Protein Summary

Product Specifications

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit IL-13-dependent proliferation of TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED₅₀ for this effect is 0.2-0.8 µg/mL in the presence of 4 ng/mL rmIL-13.

Source

Mouse myeloma cell line, NS0-derived mouse IL-13 R alpha 1 protein

Mouse IL-13 R alpha 1 (Ala25 - Thr340) Accession # O09030	DIEGR	Human IgG₁ (Pro100 - Lys330)	6-His tag
N-terminus			C-terminus

Accession #

O09030

N-terminal Sequence
Analysis

Ala25

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

63 kDa (monomer)

SDS-PAGE

100-120 kDa, reducing conditions

Product Datasheets

491-IR (with carrier)

Product Datasheet

COA

SDS

491-IR/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

491-IR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

491-IR/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 200 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: IL-13 R alpha 1

Mouse IL-13 receptor alpha one (IL‑13 R alpha 1), previously called NR4, IL-13 R alpha, and IL13R alpha ', is a member of the hemopoietin receptor family that was cloned on the basis of its conserved WSXWS motif. Mouse IL‑13 R alpha 1 encodes a 424 amino acid (aa) residue precursor type I membrane protein with a 26 aa residue signal peptide, a 314 aa residue extracellular domain (containing an immunoglobulin-like domain and a typical hemopoietin receptor domain), a 24 aa residue transmembrane region and a 60 aa residue cytoplasmic tail. Human IL‑13 R alpha 1, with 76% amino acid sequence identity to mouse IL‑13 R alpha 1, has also been cloned. In addition, a second human IL‑13 binding protein, designated IL‑13 R alpha 2 and previously known as IL‑13 R beta, IL‑13 R and IL‑13 R alpha, has been cloned from the Caki‑1 human renal carcinoma cell line. Murine IL‑13 R alpha 2 has been cloned from a mouse thymus cDNA library. The amino terminal 27 amino acid residues of the human and mouse IL‑13 R alpha 2 is nearly identical to that of a soluble mouse IL ‑3 binding proteinpurified from mouse serum and urine.

Both human and mouse IL‑13 R alpha 2 and mouse IL‑13 binding protein bind IL‑13 with high‑affinity. In cells transfected with mouse IL‑13 R alpha 1 only, low‑affinity binding with mouse IL13 is observed. However, in cells expressing both the mouse IL‑13 R alpha 1 and mouse IL4 receptor, a high affinity IL‑13 receptor complex capable of transducing an IL‑13 or IL‑4‑dependent proliferative signal, is generated.These results suggest that mouse IL‑13 R alpha 1, in addition to its role as a IL‑13 binding subunit in the IL‑13 receptor complex, can serve as an alternative to the common gamma chain for IL‑4 signaling.

References

Hilton, D.J. et al. (1996) Proc. Natl. Acad. Sci. USA 93:497.
Aman, M.J. et al. (1996) J. Biol. Chem. 271:29265.
Caput, D. et al. (1996) J. Biol. Chem. 271:16921.
Zhang, J.G. et al. (1997) J. Biol. Chem. 272:9474.
Obiri, N.I. et al. (1997) J. Immunol. 158:756.
Donaldson, D.D. et al. (1998) J. Immunol. 161:2317.

Long Name

Interleukin 13 Receptor alpha 1

Entrez Gene IDs

3597 (Human); 16164 (Mouse)

Alternate Names

bB128O4.2.1 (interleukin 13 receptor, alpha 1); Cancer/testis antigen 19; CD213a1 antigen; CD213a1; CT19; IL-13 R alpha 1; IL13 receptor alpha-1 chain; IL-13 receptor subunit alpha-1; IL13R alpha 1; IL-13R subunit alpha-1; IL13R; IL13RA; IL-13Ra; IL13RA1; IL-13Ra1; IL-13R-alpha-1; interleukin 13 receptor, alpha 1; interleukin-13 receptor subunit alpha-1; NR4