Recombinant Mouse IL-18 BPd Fc Chimera Protein, CF Summary
Product Specifications
| Mouse IL-18 BPd/Fc (Thr27 - Ala191) & (Ala29 - Ala191) Accession # AAD17194 |
IEGRMD | Human IgG1 (Pro100 - Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
122-BP
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: IL-18 BPd
Interleukin 18 binding protein (IL-18 BP) is a secreted glycoprotein, which functions as an IL-18 antagonist by binding to IL-18 and blocking its biological activity. IL-18 BP bears no amino acid sequence homology to the membrane-associated IL-18 and IL-1 receptor proteins. The gene for human IL-18 BP has been localized to chromosome 11q13. It encodes for at least four isoforms by alternative splicing. The IL-18 BP isoforms a and c each contain one immunoglobulin (Ig)-like C2-type domain while isoforms b and d lack a complete Ig domain. The complete Ig domain has been shown to be essential to the binding and neutralizing properties of the binding proteins. Two isoforms of mouse IL18 BP (c and d) containing the complete Ig domain have also been isolated and shown to neutralize IL-18 bioactivity. Human and mouse IL-18 BPs share approximately 61% amino acid sequence identity. Several poxviruses also encode proteins with sequence similarity to the human and mouse IL-18 BP. Viral IL-18 BPs have been shown to bind and inhibit IL-18 responses and may be involved in modulating host immune responses. The expression of IL-18 BP is markedly up-regulated by IFN-gamma, suggesting that IL-18 activity is modulated by a negative feedback mechanism mediated by IL-18 BP.
- Muh, H. et al. (2000) Biochem. Biophys. Res. Commun. 267:960.
- Kim, S-H. et al. (2000) Proc. Nat. Acad. Sci. USA 97:1190.
- Calderara, S. et al. (2001) Virology 279:22.
Citations for Recombinant Mouse IL-18 BPd Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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Unleashing Natural IL18 Activity Using an Anti-IL18BP Blocker Induces Potent Immune Stimulation and Antitumor Effects
Authors: Menachem, A;Alteber, Z;Cojocaru, G;Fridman Kfir, T;Blat, D;Leiderman, O;Galperin, M;Sever, L;Cohen, N;Cohen, K;Granit, RZ;Vols, S;Frenkel, M;Soffer, L;Meyer, K;Menachem, K;Galon Tilleman, H;Morein, D;Borukhov, I;Toporik, A;Perpinial Shahor, M;Tatirovsky, E;Mizrachi, A;Levy-Barda, A;Sadot, E;Strenov, Y;Eitan, R;Jakobson-Setton, A;Yanichkin, N;Ferre, P;Ophir, E;
Cancer immunology research
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Control -
Human metapneumovirus activates NOD-like receptor protein 3 inflammasome via its small hydrophobic protein which plays a detrimental role during infection in mice
Authors: VB Lê, J Dubois, C Couture, MH Cavanagh, O Uyar, A Pizzorno, M Rosa-Calat, MÈ Hamelin, G Boivin
PLoS Pathog., 2019-04-09;15(4):e1007689.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
NK cell receptor NKG2D sets activation threshold for the NCR1 receptor early in NK cell development
Authors: V Jelen?i?, M Šestan, I Kavazovi?, M Lenarti?, S Marinovi?, TD Holmes, M Prchal-Mur, B Lisni?, V Sexl, YT Bryceson, FM Wensveen, B Poli?
Nat. Immunol., 2018-09-17;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Inflammasome activation is reactive oxygen species dependent and mediates irinotecan-induced mucositis through IL-1beta and IL-18 in mice.
Authors: Arifa R, Madeira M, de Paula T, Lima R, Tavares L, Menezes-Garcia Z, Fagundes C, Rachid M, Ryffel B, Zamboni D, Teixeira M, Souza D
Am J Pathol, 2014-07-01;184(7):2023-34.
Species: Mouse
Sample Types: In Vivo
Applications: Bioassay -
Contribution of IL-17-producing gamma delta T cells to the efficacy of anticancer chemotherapy.
Authors: Ma Y, Aymeric L, Locher C, Mattarollo SR, Delahaye NF, Pereira P, Boucontet L, Apetoh L, Ghiringhelli F, Casares N, Lasarte JJ, Matsuzaki G, Ikuta K, Ryffel B, Benlagha K, Tesniere A, Ibrahim N, Dechanet-Merville J, Chaput N, Smyth MJ, Kroemer G, Zitvogel L
J. Exp. Med., 2011-03-07;208(3):491-503.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Interleukin-18 suppresses adiponectin expression in 3T3-L1 adipocytes via a novel signal transduction pathway involving ERK1/2-dependent NFATc4 phosphorylation.
Authors: Chandrasekar B, Patel DN, Mummidi S, Kim JW, Clark RA, Valente AJ
J. Biol. Chem., 2007-12-17;283(7):4200-9.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Disruption of interleukin-18, but not interleukin-1, increases vulnerability to preterm delivery and fetal mortality after intrauterine inflammation.
Authors: Wang X, Hagberg H, Mallard C, Zhu C, Hedtjarn M, Tiger CF, Eriksson K, Rosen A, Jacobsson B
Am. J. Pathol., 2006-09-01;169(3):967-76.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Application of plasmid DNA encoding IL-18 diminishes development of herpetic stromal keratitis by antiangiogenic effects.
Authors: Kim B, Lee S, Suvas S, Rouse BT
J. Immunol., 2005-07-01;175(1):509-16.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo
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