Recombinant Mouse IL-18/IL-1F4 Protein Summary
Product Specifications
Asn36-Ser192
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9139-IL
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and DTT with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
9139-IL/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and DTT with trehalose. |
| Reconstitution | Reconstitute at 100 μg/mL in PBS. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Scientific Data
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Recombinant Mouse IL-18/IL-1F4 (Catalog # 9139-IL) induces IFN gamma secretion by activated mouse T cells. The ED50 for this effect is 0.06-0.36 ng/mL.
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1 μg/lane of Recombinant Mouse IL-18 (Catalog # 9139-IL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing a single band at 18 kDa.
Reconstitution Calculator
Background: IL-18/IL-1F4
Interleukin-18 (IL-18) is a proinflammatory cytokine in the IL-1 family that exerts distinct immune effects depending on the local cytokine environment. It is expressed as a 24 kDa precursor by endothelial and epithelial cells, keratinocytes, gamma δ T cells, and phagocytes. The precursor is activated intracellularly by Caspase-1 mediated proteolysis to release the 17 kDa mature cytokine. The precursor can also be released by necrotic cells for extracellular cleavage by multiple proteases.
IL-18 activation is induced by infection or tissue damage and contributes to disease pathology in chronic inflammation (1-3). IL-18 binds to the widely expressed IL-18 R alpha which recruits IL-18 R beta to form the signaling receptor complex (4, 5). Its bioactivity is negatively regulated by interactions with IL-18 binding proteins and virally encoded IL-18BP homologs (6). In the presence of IL-12 or IL-15, IL-18 enhances anti-viral Th1 immune responses by inducing IFN-gamma production and the cytolytic activity of CD8+ T cells and NK cells (7, 8). In the absence of IL-12 or IL-15, however, IL-18 promotes production of the Th2 cytokines IL-4 and IL-13 by CD4+ T cells and basophils (9, 10). In the presence of IL-1 beta or IL-23, IL-18 induces the antigen-independent production of IL-17 by gamma δ T cells and CD4+ T cells (11). IL-18 also promotes myeloid dendritic cell maturation and triggers neutrophil respiratory burst (12, 13). In cancer, IL-18 exhibits diverse activities including enhancing anti-tumor immunity, inhibiting or promoting angiogenesis, and promoting tumor cell metastasis (14). Mature mouse IL-18 shares 63% and 91% amino acid sequence identity with mouse and rat IL-18, respectively (15).
- Dinarello, C.A. et al. (2013) Front. Immunol. 4:289.
- Smith, D.E. (2011) J. Leukoc. Biol. 89:383.
- Gu, Y. et al. (1997) Science 275:206.
- Torigoe, K. et al. (1997) J. Biol. Chem. 272:25737.
- Cheung, H. et al. (2005) J. Immunol. 174:5351.
- Novick, D. et al. (1999) Immunity 10:127.
- Fehniger, T.A. et al. (1999) J. Immunol. 162:4511.
- Yoshimoto, T. et al. (1998) J. Immunol. 161:3400.
- Yoshimoto, T. et al. (2000) Nat. Immunol. 1:132.
- Kroeger, K.M. et al. (2009) J. Leukoc. Biol. 86:769.
- Lalor, S.J. et al. (2011) J. Immunol. 186:5738.
- Li, J. et al. (2004) Cell. Immunol. 227:103.
- Elbim, C. et al. (2005) Clin. Diagn. Lab. Immunol. 12:436.
- Fabbi, M. et al. (2015) J. Leukoc. Biol. 97:665.
- Okamura, H. et al. (1995) Nature 378:88.
Citations for Recombinant Mouse IL-18/IL-1F4 Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Type 1 immunity enables neonatal thymic ILC1 production
Authors: Tougaard, P;Pérez, MR;Steels, W;Huysentruyt, J;Verstraeten, B;Vetters, J;Divert, T;Gonçalves, A;Roelandt, R;Takahashi, N;Janssens, S;Buus, TB;Taghon, T;Leclercq, G;Vandenabeele, P;
Science advances
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
An IL-4 signalling axis in bone marrow drives pro-tumorigenic myelopoiesis
Authors: LaMarche, NM;Hegde, S;Park, MD;Maier, BB;Troncoso, L;Le Berichel, J;Hamon, P;Belabed, M;Mattiuz, R;Hennequin, C;Chin, T;Reid, AM;Reyes-Torres, I;Nemeth, E;Zhang, R;Olson, OC;Doroshow, DB;Rohs, NC;Gomez, JE;Veluswamy, R;Hall, N;Venturini, N;Ginhoux, F;Liu, Z;Buckup, M;Figueiredo, I;Roudko, V;Miyake, K;Karasuyama, H;Gonzalez-Kozlova, E;Gnjatic, S;Passegué, E;Kim-Schulze, S;Brown, BD;Hirsch, FR;Kim, BS;Marron, TU;Merad, M;
Nature
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Cytokine-Mediated Degradation of the Transcription Factor ERG Impacts the Pulmonary Vascular Response to Systemic Inflammatory Challenge
Authors: CM Schafer, S Martin-Alm, K Kurylowicz, N Dufton, L Osuna-Alma, ML Wu, CF Johnson, AV Shah, DO Haskard, A Buxton, E Willis, K Wheeler, S Turner, M Chlebicz, RP Scott, S Kovats, A Cleuren, GM Birdsey, AM Randi, CT Griffin
bioRxiv : the preprint server for biology, 2023-02-11;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: Bioassay -
In�vivo G-CSF treatment activates the GR-SOCS1 axis to suppress IFN-gamma secretion by natural killer cells
Authors: X Zhao, T Peng, X Cao, Y Hou, R Li, T Han, Z Fan, M Zhao, Y Chang, H Chen, C Li, X Huang
Cell Reports, 2022-09-13;40(11):111342.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
IL-22 initiates an IL-18-dependent epithelial response circuit to enforce intestinal host defence
Authors: HY Chiang, HH Lu, JN Sudhakar, YW Chen, NS Shih, YT Weng, JW Shui
Nature Communications, 2022-02-15;13(1):874.
Species: Mouse
Sample Types: Organoids
Applications: Bioassay -
Interleukin-18 accelerates cardiac inflammation and dysfunction during ischemia/reperfusion injury by transcriptional activation of CXCL16
Authors: G Zhao, H Zhang, S Zhu, S Wang, K Zhu, Y Zhao, L Xu, P Zhang, J Xie, A Sun, Y Zou, J Ge
Cellular Signalling, 2021-09-04;0(0):110141.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Chronic stress physically spares but functionally impairs innate-like invariant T�cells
Authors: PT Rudak, J Choi, KM Parkins, KL Summers, DN Jackson, PJ Foster, AI Skaro, K Leslie, VC McAlister, VK Kuchroo, W Inoue, O Lantz, SMM Haeryfar
Cell Reports, 2021-04-13;35(2):108979.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
ILC2s amplify PD-1 blockade by activating tissue-specific cancer immunity
Authors: JA Moral, J Leung, LA Rojas, J Ruan, J Zhao, Z Sethna, A Ramnarain, B Gasmi, M Gururajan, D Redmond, G Askan, U Bhanot, E Elyada, Y Park, DA Tuveson, M Gönen, SD Leach, JD Wolchok, RP DeMatteo, T Merghoub, VP Balachandr
Nature, 2020-02-19;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Bystander Activation of Pulmonary Trm Cells Attenuates the Severity of Bacterial Pneumonia by Enhancing Neutrophil Recruitment
Authors: C Ge, IR Monk, A Pizzolla, N Wang, JG Bedford, TP Stinear, GP Westall, LM Wakim
Cell Rep, 2019-12-24;29(13):4236-4244.e3.
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture -
Polymicrobial sepsis influences NK-cell-mediated immunity by diminishing NK-cell-intrinsic receptor-mediated effector responses to viral ligands or infections
Authors: IJ Jensen, CS Winborn, MG Fosdick, P Shao, MM Tremblay, Q Shan, SK Tripathy, CM Snyder, HH Xue, TS Griffith, JC Houtman, VP Badovinac
PLoS Pathog., 2018-10-31;14(10):e1007405.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Activin in acute pancreatitis: Potential risk-stratifying marker and novel therapeutic target.
Authors: Jonas J Staudacher, Cemal Yazici, Timothy Carroll, Jessica Bauer, Jingbo Pang, Nancy Krett, Yinglin Xia, Annette Wilson, Georgios Papachris, Andrea Dirmeier, Claudia Kunst, David C Whitcomb, Giamila Fantuzzi, Barbara Jung
Scientific Reports, 2017-10-06;0(0):2045-2322.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo
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