Recombinant Mouse IL-18/IL-1F4 Protein, CF
Recombinant Mouse IL-18/IL-1F4 Protein, CF Summary
Product Specifications
36-192 aa
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
B004-5
| Formulation | Lyophilized in PBS containing 1% sucrose. |
| Reconstitution | Reconstitute in 250 μL of ice-cold distilled water on ice. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date. |
Reconstitution Calculator
Background: IL-18/IL-1F4
Interleukin-18 (IL-18) is a proinflammatory cytokine in the IL-1 family that exerts distinct immune effects depending on the local cytokine environment. It is expressed as a 24 kDa precursor by endothelial and epithelial cells, keratinocytes, gamma δ T cells, and phagocytes. The precursor is activated intracellularly by Caspase-1 mediated proteolysis to release the 17 kDa mature cytokine. The precursor can also be released by necrotic cells for extracellular cleavage by multiple proteases.
IL-18 activation is induced by infection or tissue damage and contributes to disease pathology in chronic inflammation (1-3). IL-18 binds to the widely expressed IL-18 R alpha which recruits IL-18 R beta to form the signaling receptor complex (4, 5). Its bioactivity is negatively regulated by interactions with IL-18 binding proteins and virally encoded IL-18BP homologs (6). In the presence of IL-12 or IL-15, IL-18 enhances anti-viral Th1 immune responses by inducing IFN-gamma production and the cytolytic activity of CD8+ T cells and NK cells (7, 8). In the absence of IL-12 or IL-15, however, IL-18 promotes production of the Th2 cytokines IL-4 and IL-13 by CD4+ T cells and basophils (9, 10). In the presence of IL-1 beta or IL-23, IL-18 induces the antigen-independent production of IL-17 by gamma δ T cells and CD4+ T cells (11). IL-18 also promotes myeloid dendritic cell maturation and triggers neutrophil respiratory burst (12, 13). In cancer, IL-18 exhibits diverse activities including enhancing anti-tumor immunity, inhibiting or promoting angiogenesis, and promoting tumor cell metastasis (14). Mature mouse IL-18 shares 63% and 91% amino acid sequence identity with mouse and rat IL-18, respectively (15).
- Dinarello, C.A. et al. (2013) Front. Immunol. 4:289.
- Smith, D.E. (2011) J. Leukoc. Biol. 89:383.
- Gu, Y. et al. (1997) Science 275:206.
- Torigoe, K. et al. (1997) J. Biol. Chem. 272:25737.
- Cheung, H. et al. (2005) J. Immunol. 174:5351.
- Novick, D. et al. (1999) Immunity 10:127.
- Fehniger, T.A. et al. (1999) J. Immunol. 162:4511.
- Yoshimoto, T. et al. (1998) J. Immunol. 161:3400.
- Yoshimoto, T. et al. (2000) Nat. Immunol. 1:132.
- Kroeger, K.M. et al. (2009) J. Leukoc. Biol. 86:769.
- Lalor, S.J. et al. (2011) J. Immunol. 186:5738.
- Li, J. et al. (2004) Cell. Immunol. 227:103.
- Elbim, C. et al. (2005) Clin. Diagn. Lab. Immunol. 12:436.
- Fabbi, M. et al. (2015) J. Leukoc. Biol. 97:665.
- Okamura, H. et al. (1995) Nature 378:88.
Citations for Recombinant Mouse IL-18/IL-1F4 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Suppression of adaptive NK cell expansion by macrophage-mediated phagocytosis inhibited by 2B4-CD48
Authors: Li, R;Galindo, CC;Davidson, D;Guo, H;Zhong, MC;Qian, J;Li, B;Ruzsics, Z;Lau, CM;O'Sullivan, TE;Vidal, SM;Sun, JC;Veillette, A;
Cell reports
Species: Mouse, Transgenic Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Interleukin-22 induces interleukin-18 expression from epithelial cells during intestinal infection.
Authors: Munoz M, Eidenschenk C, Ota N, Wong K, Lohmann U, Kuhl A, Wang X, Manzanillo P, Li Y, Rutz S, Zheng Y, Diehl L, Kayagaki N, van Lookeren-Campagne M, Liesenfeld O, Heimesaat M, Ouyang W
Immunity, 2015-02-10;42(2):321-31.
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay -
IL-18 is a key proximal mediator of contact hypersensitivity and allergen-induced Langerhans cell migration in murine epidermis.
Authors: Antonopoulos C, Cumberbatch M, Mee JB, Dearman RJ, Wei XQ, Liew FY, Kimber I, Groves RW
J. Leukoc. Biol., 2007-11-05;83(2):361-7.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
STAT4 serine phosphorylation is critical for IL-12-induced IFN-gamma production but not for cell proliferation.
Authors: 107335, O'shea JJ
MAD2L2 promotes replication fork protection and recovery in a shieldin-independent and REV3L-dependent manner, 2002-09-04;99(19):12281-6.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: Bioassay
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