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Recombinant Mouse IL-18/IL-1F4 Protein

Catalog #: B002-5
35 Citations Datasheet / COA / SDS
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B002-5
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Recombinant Mouse IL-18/IL-1F4 Protein Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Induction of IFN-gamma by Mouse IL-18 receptor transfected KG-1 cell [human myelomonocyte; ATCC CCL246] in response to the recombinant mouse IL-18 was measured using human IFN-gamma ELISA. The activity of lot 040 was as follows:IL-18 final concentration 5ng/mL; IFN-gamma induction 2863 IU/mL and IL-18 final concentration 10 ng/mL; IFN-gamma induction 7312 IU/mL.
Source
E. coli-derived mouse IL-18/IL-1F4 protein
36-192 aa
Predicted Molecular Mass
18 kDa

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B002-5

Product Datasheet
COA
SDS

B002-5

Formulation 25 μg in PBS containing 0.1% BSA and 1% sucrose.
Reconstitution  Reconstitute in 250 μL of ice-cold distilled water on ice.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
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Background: IL-18/IL-1F4

Interleukin-18 (IL-18) is a proinflammatory cytokine in the IL-1 family that exerts distinct immune effects depending on the local cytokine environment. It is expressed as a 24 kDa precursor by endothelial and epithelial cells, keratinocytes, gamma δ T cells, and phagocytes. The precursor is activated intracellularly by Caspase-1 mediated proteolysis to release the 17 kDa mature cytokine. The precursor can also be released by necrotic cells for extracellular cleavage by multiple proteases.
IL-18 activation is induced by infection or tissue damage and contributes to disease pathology in chronic inflammation (1-3). IL-18 binds to the widely expressed IL-18 R alpha which recruits IL-18 R beta to form the signaling receptor complex (4, 5). Its bioactivity is negatively regulated by interactions with IL-18 binding proteins and virally encoded IL-18BP homologs (6). In the presence of IL-12 or IL-15, IL-18 enhances anti-viral Th1 immune responses by inducing IFN-gamma production and the cytolytic activity of CD8+ T cells and NK cells (7, 8). In the absence of IL-12 or IL-15, however, IL-18 promotes production of the Th2 cytokines IL-4 and IL-13 by CD4+ T cells and basophils (9, 10). In the presence of IL-1 beta or IL-23, IL-18 induces the antigen-independent production of IL-17 by gamma δ T cells and CD4+ T cells (11). IL-18 also promotes myeloid dendritic cell maturation and triggers neutrophil respiratory burst (12, 13). In cancer, IL-18 exhibits diverse activities including enhancing anti-tumor immunity, inhibiting or promoting angiogenesis, and promoting tumor cell metastasis (14). Mature mouse IL-18 shares 63% and 91% amino acid sequence identity with mouse and rat IL-18, respectively (15).

References
  1. Dinarello, C.A. et al. (2013) Front. Immunol. 4:289.
  2. Smith, D.E. (2011) J. Leukoc. Biol. 89:383.
  3. Gu, Y. et al. (1997) Science 275:206.
  4. Torigoe, K. et al. (1997) J. Biol. Chem. 272:25737.
  5. Cheung, H. et al. (2005) J. Immunol. 174:5351.
  6. Novick, D. et al. (1999) Immunity 10:127.
  7. Fehniger, T.A. et al. (1999) J. Immunol. 162:4511.
  8. Yoshimoto, T. et al. (1998) J. Immunol. 161:3400.
  9. Yoshimoto, T. et al. (2000) Nat. Immunol. 1:132.
  10. Kroeger, K.M. et al. (2009) J. Leukoc. Biol. 86:769.
  11. Lalor, S.J. et al. (2011) J. Immunol. 186:5738.
  12. Li, J. et al. (2004) Cell. Immunol. 227:103.
  13. Elbim, C. et al. (2005) Clin. Diagn. Lab. Immunol. 12:436.
  14. Fabbi, M. et al. (2015) J. Leukoc. Biol. 97:665.
  15. Okamura, H. et al. (1995) Nature 378:88.
Long Name
Interleukin 18
Entrez Gene IDs
3606 (Human); 16173 (Mouse); 29197 (Rat); 397057 (Porcine); 574151 (Primate)
Alternate Names
Iboctadekin; IFN-gamma-inducing factor; IGIF; IGIFIL-1 gamma; IL18; IL-18; IL-18MGC12320; IL-1F4; IL1F4iboctadekin; IL-1g; Interferon gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor); Interleukin-1 gamma; interleukin-18

Citations for Recombinant Mouse IL-18/IL-1F4 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

35 Citations: Showing 1 - 10
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  1. Serum amyloid A-mediated inflammasome activation of microglial cells in cerebral ischemia
    Authors: J Yu, H Zhu, S Taheri, W Mondy, L Bonilha, GS Magwood, D Lackland, RJ Adams, MS Kindy
    J. Neurosci., 2019-10-14;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Standard
  2. Helicobacter pylori Exploits the NLRC4 Inflammasome to Dampen Host Defenses
    Authors: RP Semper, M Vieth, M Gerhard, R Mejías-Luq
    J. Immunol., 2019-09-11;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. KLF12 Regulates Mouse NK Cell Proliferation
    Authors: VC Lam, L Folkersen, OA Aguilar, LL Lanier
    J. Immunol., 2019-07-12;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Mouse cytomegalovirus-experienced ILC1s acquire a memory response dependent on the viral glycoprotein m12
    Authors: OE Weizman, E Song, NM Adams, AD Hildreth, L Riggan, C Krishna, OA Aguilar, CS Leslie, JR Carlyle, JC Sun, TE O'Sullivan
    Nat. Immunol., 2019-07-01;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Soy isoflavones and their metabolites modulate cytokine-induced natural killer cell function
    Authors: TA Mace, MB Ware, SA King, S Loftus, MR Farren, E McMichael, S Scoville, C Geraghty, G Young, WE Carson, SK Clinton, GB Lesinski
    Sci Rep, 2019-03-25;9(1):5068.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  6. IL-27 promotes NK cell effector functions via Maf-Nrf2 pathway during influenza infection
    Authors: P Kumar, K Rajasekara, A Nanbakhsh, J Gorski, MS Thakar, S Malarkanna
    Sci Rep, 2019-03-21;9(1):4984.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  7. The NLRP3 Inflammasome Suppresses Protective Immunity to Gastrointestinal Helminth Infection
    Authors: R Alhallaf, Z Agha, CM Miller, AAB Robertson, J Sotillo, J Croese, MA Cooper, SL Masters, A Kupz, NC Smith, A Loukas, PR Giacomin
    Cell Rep, 2018-04-24;23(4):1085-1098.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  8. Altered expression of IL-18 binding protein and IL-18 receptor in basophils and mast cells of asthma patients
    Authors: Z Wang, Z Liu, L Wang, J Wang, L Chen, H Xie, H Zhang, S He
    Scand. J. Immunol., 2018-04-10;0(0):.
    Species: Mouse
    Sample Types: Plasma
    Applications: ELISA (Standard)
  9. Crk Adaptor Proteins Regulate NK Cell Expansion and Differentiation during Mouse Cytomegalovirus Infection
    Authors: T Nabekura, Z Chen, C Schroeder, T Park, E Vivier, LL Lanier, D Liu
    J. Immunol., 2018-04-04;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Protected graft copolymer-formulated fibroblast growth factors mitigate the lethality of partial body irradiation injury
    Authors: GM Castillo, A Nishimoto-, CC Jones, KK Kabirov, A Zakharov, AV Lyubimov
    PLoS ONE, 2017-02-16;12(2):e0171703.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA (Standard)
  11. Glial-cell-derived neuroregulators control type 3 innate lymphoid cells and gut defence
    Nature, 2016-07-13;535(7612):440-3.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Dissecting CD8+ NKT Cell Responses to Listeria Infection Reveals a Component of Innate Resistance.
    Authors: Seregin S, Chen G, Laouar Y
    J Immunol, 2015-06-26;195(3):1112-20.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  13. IL-33 receptor ST2 amplifies the expansion of NK cells and enhances host defense during mouse cytomegalovirus infection.
    Authors: Nabekura T, Girard J, Lanier L
    J Immunol, 2015-04-29;194(12):5948-52.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  14. NK cells require IL-28R for optimal in vivo activity.
    Authors: Souza-Fonseca-Guimaraes F, Young A, Mittal D, Martinet L, Bruedigam C, Takeda K, Andoniou C, Degli-Esposti M, Hill G, Smyth M
    Proc Natl Acad Sci U S A, 2015-04-21;112(18):E2376-84.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  15. IL-18 acts in synergy with IL-7 to promote ex vivo expansion of T lymphoid progenitor cells.
    Authors: Gandhapudi S, Tan C, Marino J, Taylor A, Pack C, Gaikwad J, Van De Wiele C, Wren J, Teague T
    J Immunol, 2015-03-16;194(8):3820-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Genetic investigation of MHC-independent missing-self recognition by mouse NK cells using an in vivo bone marrow transplantation model.
    Authors: Chen P, Aguilar O, Rahim M, Allan D, Fine J, Kirkham C, Ma J, Tanaka M, Tu M, Wight A, Kartsogiannis V, Gillespie M, Makrigiannis A, Carlyle J
    J Immunol, 2015-02-13;194(6):2909-18.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Protective role for caspase-11 during acute experimental murine colitis.
    Authors: Oficjalska K, Raverdeau M, Aviello G, Wade S, Hickey A, Sheehan K, Corr S, Kay E, O'Neill L, Mills K, Creagh E
    J Immunol, 2014-12-29;194(3):1252-60.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  18. The metabolic checkpoint kinase mTOR is essential for IL-15 signaling during the development and activation of NK cells.
    Authors: Marcais A, Cherfils-Vicini J, Viant C, Degouve S, Viel S, Fenis A, Rabilloud J, Mayol K, Tavares A, Bienvenu J, Gangloff Y, Gilson E, Vivier E, Walzer T
    Nat Immunol, 2014-06-29;15(8):749-57.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  19. Dok1 and Dok2 proteins regulate natural killer cell development and function.
    Authors: Celis-Gutierrez J, Boyron M, Walzer T, Pandolfi P, Jonjic S, Olive D, Dalod M, Vivier E, Nunes J
    EMBO J, 2014-06-24;33(17):1928-40.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  20. Anti-inflammatory cytokines directly inhibit innate but not adaptive CD8+ T cell functions.
    Authors: Freeman B, Meyer C, Slifka M
    J Virol, 2014-04-16;88(13):7474-84.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  21. CD4(+) T cells from food allergy model are resistant to TCR-dependent apoptotic induction.
    Authors: He W, Yang C, Xia L, Zhao M, Ge R, Huang H, Ji Q, Yang P, Liu Z
    Cytokine, 2014-04-16;68(1):32-9.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  22. The receptors CD96 and CD226 oppose each other in the regulation of natural killer cell functions.
    Authors: Chan, Christop, Martinet, Ludovic, Gilfillan, Susan, Souza-Fonseca-Guimaraes, Fernando, Chow, Melvyn T, Town, Liam, Ritchie, David S, Colonna, Marco, Andrews, Daniel M, Smyth, Mark J
    Nat Immunol, 2014-03-23;15(5):431-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  23. Polymicrobial sepsis alters antigen-dependent and -independent memory CD8 T cell functions.
    Authors: Duong S, Condotta S, Rai D, Martin M, Griffith T, Badovinac V
    J Immunol, 2014-03-19;192(8):3618-25.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  24. Distinct requirements for activation of NKT and NK cells during viral infection.
    Authors: Tyznik A, Verma S, Wang Q, Kronenberg M, Benedict C
    J Immunol, 2014-03-14;192(8):3676-85.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  25. Essential role of CD11a in CD8+ T-cell accumulation and activation in adipose tissue.
    Authors: Jiang E, Perrard X, Yang D, Khan I, Perrard J, Smith C, Ballantyne C, Wu H
    Arterioscler Thromb Vasc Biol, 2013-10-24;34(1):34-43.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  26. Impaired bone formation and increased osteoclastogenesis in mice lacking chemokine (C-C motif) ligand 5 (Ccl5).
    Authors: Wintges K, Beil F, Albers J, Jeschke A, Schweizer M, Claass B, Tiegs G, Amling M, Schinke T
    J Bone Miner Res, 2013-10-01;28(10):2070-80.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  27. IL-18 regulates melanoma VLA-4 integrin activation through a Hierarchized sequence of inflammatory factors.
    Authors: Valcarcel M, Carrascal T, Crende O, Vidal-Vanaclocha F
    J Invest Dermatol, 2013-08-12;134(2):470-80.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  28. Regulation of innate CD8+ T-cell activation mediated by cytokines.
    Proc Natl Acad Sci U S A, 2012-06-04;109(25):9971-6.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  29. Interleukin-1 family cytokines as mucosal vaccine adjuvants for induction of protective immunity against influenza virus.
    Authors: Kayamuro H, Yoshioka Y, Abe Y, Arita S, Katayama K, Nomura T, Yoshikawa T, Kubota-Koketsu R, Ikuta K, Okamoto S, Mori Y, Kunisawa J, Kiyono H, Itoh N, Nagano K, Kamada H, Tsutsumi Y, Tsunoda S
    J. Virol., 2010-09-29;84(24):12703-12.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  30. IL-18 induces a marked gene expression profile change and increased Ccl1 (I-309) production in mouse mucosal mast cell homologs.
    Authors: Wiener Z, Pocza P, Racz M, Nagy G, Tolgyesi G, Molnar V, Jaeger J, Buzas E, Gorbe E, Papp Z, Rigo J, Falus A
    Int. Immunol., 2008-10-21;20(12):1565-73.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  31. Interleukin-18 suppresses adiponectin expression in 3T3-L1 adipocytes via a novel signal transduction pathway involving ERK1/2-dependent NFATc4 phosphorylation.
    Authors: Chandrasekar B, Patel DN, Mummidi S, Kim JW, Clark RA, Valente AJ
    J. Biol. Chem., 2007-12-17;283(7):4200-9.
    Species: Mouse
    Sample Types: In Vivo, Whole Cells
    Applications: Bioassay, In Vivo
  32. IL-18 is a key proximal mediator of contact hypersensitivity and allergen-induced Langerhans cell migration in murine epidermis.
    Authors: Antonopoulos C, Cumberbatch M, Mee JB, Dearman RJ, Wei XQ, Liew FY, Kimber I, Groves RW
    J. Leukoc. Biol., 2007-11-05;83(2):361-7.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  33. T helper 1 cells stimulated with ovalbumin and IL-18 induce airway hyperresponsiveness and lung fibrosis by IFN-gamma and IL-13 production.
    Authors: Hayashi N, Yoshimoto T, Izuhara K, Matsui K, Tanaka T, Nakanishi K
    Proc. Natl. Acad. Sci. U.S.A., 2007-08-31;104(37):14765-70.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  34. DAP12 signaling directly augments proproliferative cytokine stimulation of NK cells during viral infections.
    Authors: French AR, Sjolin H, Kim S, Koka R, Yang L, Young DA, Cerboni C, Tomasello E, Ma A, Vivier E, Karre K, Yokoyama WM
    J. Immunol., 2006-10-15;177(8):4981-90.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  35. Control of Leishmania major in the absence of Tyk2 kinase.
    Authors: Schleicher U, Mattner J, Blos M, Schindler H, Rollinghoff M, Karaghiosoff M, Muller M, Werner-Felmayer G, Bogdan C
    Eur. J. Immunol., 2004-02-01;34(2):519-29.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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