Recombinant Mouse IL-18 R alpha/IL-1 R5 Fc Chimera, CF

Catalog #: 9285-BP Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9285-BP-100

Recombinant Mouse IL-18 R alpha/IL-1 R5 Fc Chimera Bioactivity

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All IL-18 R alpha/IL-1 R5 Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse IL-18 R alpha/IL-1 R5 Fc Chimera, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse IL‑18 R alpha /IL‑1 R5 Chimera is immobilized at 2 μg/mL, 100 μL/well, the concentration of Recombinant Mouse IL-18 (Catalog # 9139-CL) that produces 50% of the optimal binding response is 1.5-9 μg/mL.

Source

Mouse myeloma cell line, NS0-derived mouse IL-18 R alpha/IL-1 R5 protein

Mouse IL-18 R alpha/IL-1 R5 (Lys20-Gly326) Accession # Q61098	IEGRDP	Mouse IgG_2a (Glu98-Lys330)
N-terminus		C-terminus

Accession #

Q61098

N-terminal Sequence
Analysis

Lys20

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

62 kDa

SDS-PAGE

90-106 kDa, reducing conditions

Product Datasheets

9285-BP

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9285-BP

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 1 mg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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When Recombinant Mouse IL-18 Ra/IL-1 R5 Fc Chimera (Catalog # 9285-BP) is coated at 2 µg/mL, 100 µL/well, Recombinant Mouse IL-18/IL-1F4 (Catalog # 9139-IL) binds with a ED₅₀ of 1.5-9 µg/mL.

Reconstitution Calculator

Background: IL-18 R alpha/IL-1 R5

IL-18 Receptor alpha (IL-18 Ra), also known as IL-18 R1, IL-1 R5, and IL-1 Rrp, is a transmembrane component of the IL-18 Receptor (1). It associates with IL‑18 Rb/IL‑1 R7 to form the high affinity receptor complex for IL-18 and confer cellular responsiveness to IL-18 (2, 3). IL-18 is a member of the IL-1 family of cytokines and shares numerous immunoregulatory functions with IL-12. Mature mouse IL-18 Ra consists of a 307 amino acid (aa) extracellular domain (ECD) with three immunoglobulin-like domains, a 21 aa transmembrane segment, and a 190 aa cytoplasmic domain (4). Within the ECD, mouse IL-18 Ra shares 56% and 82% aa sequence identity with human and rat IL-18 Ra, respectively. IL-18 Ra is widely expressed on epithelial cells as well as on B cells, NK cells, CD4⁺ and CD8⁺ T cells, and dendritic cells (5-8). Signaling through the IL-18 receptor promotes a Th1 biased immune response and NK cell cytolytic activity (6, 7, 9-11). It also contributes to cigarette smoke-induced airway inflammation and epithelial damage (12).

References

Dinarello, C.A. et al. (2013) Front. Immunol. 4:289.
Torigoe, K. et al. (1997) J. Biol. Chem. 272:25737.
Born, T.L. et al. (1998) J. Biol. Chem. 273:29445.
Parnet, P. et al. (1996) J. Biol. Chem. 271:3967.
Kunikata, T. et al. (1998) Cell Immunol. 189:135.
Smeltz, R.B. et al. (2001) J. Exp. Med. 194:143.
Chan, W.L. et al. (2001) J. Immunol. 167:1238.
Kaser, A. et al. (2004) Blood 103:648.
Tomura, M. et al. (1998) J. Immunol. 160:3759.
Yoshimoto, T. et al. (1998) J. Immunol. 161:3400.
Hyodo, Y. et al. (1999) J. Immunol. 162:1662.
Kang, M.J. et al. (2007) J. Immunol. 178:1948.

Long Name

Interleukin 18 Receptor alpha

Entrez Gene IDs

8809 (Human); 16182 (Mouse)

Alternate Names

CD218a; CD218a;CDw218a;IL18R1;IL18RA;IL-18Ralpha;IL-18R-alpha;IL18Ralpha2;IL1RRP;IL-1Rrp;Interleukin-18 receptor 1; IL-1 R5; IL-1 Rrp; IL-18 R alpha; IL-18 R1; IL18R alpha; IL18R1; IL-18Ra