Recombinant Mouse IL-22BP Fc Chimera Protein, CF

Catalog #: 2376-BP
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
2376-BP-025

R&D Systems Recombinant Proteins and Enzymes

1 Image

Product Details

Citations (1)

Supplemental Products

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse IL-22BP Fc Chimera Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit IL-22-induced IL-10 secretion by COLO 205 human colorectal adenocarcinoma cells. The ED₅₀ for this effect is 1-4 μg/mL in the presence of 0.5 ng/mL of Recombinant Mouse IL-22 (Catalog # 582-ML).

Source

Mouse myeloma cell line, NS0-derived mouse IL-22BP protein

Mouse IL-22BP (Thr19-Pro230) Accession # Q08AV1	IEGRMDP	Mouse IgG_2A (Glu98-Lys330)
N-terminus		C-terminus

Accession #

Q08AV1

N-terminal Sequence
Analysis

Thr19

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

51.8 kDa (monomer)

SDS-PAGE

60-70 kDa, reducing conditions

Product Datasheets

2376-BP

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2376-BP

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 250 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: IL-22BP

Interleukin 22 binding protein (IL-22BP), also known as CRF2-10, CRF2-X, and IL-22 RA2, is a 35-45 kDa secreted glycoprotein in the type II cytokine receptor family (CRF). IL-22 signals through a receptor complex consisting of IL-22 R and IL-10 R beta. IL-10 R beta is also a component of the receptor complexes for IL-10, IL-26, IL-28, and IL-29 (1, 2). IL-22BP blocks the interaction of IL-22 with IL-22 R, preventing IL-22 induced production of reactive oxygen species, IL-6, IL-10, and TNF-a (3-8). In vivo, it regulates the proinflammatory effects of IL-22 (e.g. neutrophil infiltration) but not of IL-10 (7). Mouse IL-22BP can neutralize the bioactivity of both mouse and human IL-22 (6). IL-22BP is produced by dendritic cells (DE), epithelial cells, activated B cells, and activated monocytes (3, 6, 9, 10). It is constitutively expressed by DC but is down-regulated during local inflammation and in response to tissue damage (11-13). IL-22BP is critical for limiting IL-22 induced epithelial cell proliferation during wound healing, and its deficiency can enable uncontrolled proliferation and enhance tumor development (12). Mature mouse IL-22BP consists of two tandem Fibronectin type-III domains and shares 85% and 68% amino acid sequence identity with rat IL-22BP and the short isoform of human IL-22BP, respectively (6, 14).

References

Lim, C. and R. Savan (2014) Cytokine Growth Factor Rev. 25:257.
Mizoguchi, A. (2012) Inflamm. Bowel Dis. 18:1777.
Xu, W. et al. (2001) Proc. Natl. Acad. Sci. USA 98:9511.
Kotenko, S.V. et al. (2001) J. Immunol. 166:7096.
Li, J. et al. (2004) Int. Immunopharmacol. 4:693.
Wei, C.-C. et al. (2003) Genes Immun. 4:204.
Weber, G.F. et al. (2007) Infect. Immun. 75:1690.
Whittington, H.A. et al. (2004) Am. J. Respir. Cell Mol. Biol. 31:220.
Lecart, S. et al. (2002) Int. Immunol. 14:1351.
Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:577.
Wolk, K. et al. (2007) J. Immunol. 178:5973.
Huber, S. et al. (2012) Nature 491:259.
Martin, J.C.J. et al. (2014) Mucosal Immunol. 7:101.
Weiss, B. et al. (2004) Genes Immun. 5:330.

Long Name

Interleukin 22 Binding Protein

Entrez Gene IDs

116379 (Human); 237310 (Mouse)

Alternate Names

class II cytokine receptor; CRF2-10; CRF2-S1IL22BP; CRF2-X; Cytokine receptor class-II member 10; Cytokine receptor family 2 member 10; Cytokine receptor family type 2, soluble 1; IL-22 RA2; IL-22 receptor subunit alpha-2; IL22BP; IL-22BP; IL-22BPCRF2X; IL22RA2; IL-22RA2; IL-22R-alpha-2; interleukin 22 receptor, alpha 2; interleukin 22-binding protein; interleukin-22 receptor subunit alpha-2; Interleukin-22-binding protein; MGC150509; MGC150510; zcytoR16

Related Research Areas

Citation for Recombinant Mouse IL-22BP Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1