Recombinant Mouse IL-31RA Fc Chimera Protein, CF

Name: Recombinant Mouse IL-31RA Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 1253-IL
Rating: 5 (1 reviews)

Catalog #: 1253-IL 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
1253-IL-050

Recombinant Mouse IL-31RA Fc Chimera Protein SDS-PAGE

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All IL-31RA Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse IL-31RA Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse IL‑31 RA Fc Chimera is immobilized at 5 µg/mL (100 µL/well), Recombinant Mouse IL‑31 (Catalog # 3028-ML) binds with an ED₅₀ of 0.9-5.4 μg/mL.

Source

Spodoptera frugiperda, Sf 21 (stably transfected)-derived mouse IL-31 RA protein

Mouse IL-31RA (Val19-Ser503) Accession # AAM27959.1	DIEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

AAM27959.1

N-terminal Sequence
Analysis

Val19

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

82 kDa

SDS-PAGE

90-103 kDa, reducing conditions

Product Datasheets

1253-IL

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1253-IL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	12 months from date of receipt, ≤ -20 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Scientific Data

SDS-PAGE

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1 μg/lane of Recombinant Mouse IL‑31 RA was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 90-103 kDa and 180-210 kDa, respectively.

Reconstitution Calculator

Background: IL-31RA

The interleukin-31 receptor A subunit (IL-31 RA), also known as gp130-Like Monocyte Receptor (GLM-R or GPL), is a ~100 kDa type I transmembrane glycoprotein that is classified as being a type I cytokine receptor (1, 2). A heterodimeric complex of IL-31 RA and the oncostatin M receptor (OSM R) functions as the signaling receptor for IL-31 (3). Both subunits are inducibly expressed throughout the myelomonocytic lineage and are up-regulated by interferon-gamma and bacterial lipopolysaccharides (1-3). IL-31 RA is also expressed on keratinocytes, dorsal root ganglia neurons, and variably on lung epithelial cells (3-6). The 716 amino acid (aa) mouse IL-31 RA contains an 18 aa signal sequence, a 481 aa extracellular domain (ECD), a 21 aa transmembrane domain and a 196 aa cytoplasmic domain. The ECD of mouse IL-31 RA shares 60% and 84% aa identity with human and rat IL-31 RA ECD, respectively. Mouse IL-31 receptors do not respond to human IL-31 (7). The ECD contains five fibronectin type III domains; the first two contain four conserved cysteine residues and a WSXWS motif common to type I cytokine receptors (2). In both humans and transgenic mice, IL-31 from skin-homing Th2 cells may contribute to the pruritis (itching) associated with nonatopic dermatitis, especially in infected skin (3, 8, 9).

References

Ghilardi, N. et al. (2002) J. Biol. Chem. 277:16831.
Diveu, C. et al. (2003) J. Biol. Chem. 278:49850.
Dillon, S. R. et al. (2004) Nat. Immunol. 5:752.
Chattopadhyay, S. et al. (2007) J. Biol. Chem. 282:3014.
Perrigoue, J. G. et al. (2007) J. Exp. Med. 204:481.
Bando, T. et al. (2006) Neuroscience 142:1263.
Broxmeyer, H. E. et al. (2007) Exp. Hematol. 35:78.
Bilsborough, J. et al. (2006) J. Allergy Clin. Immunol. 117:418.
Sonkoly, E. et al. (2006) J. Allergy Clin. Immunol. 117:411.

Long Name

Interleukin 31 Receptor A

Entrez Gene IDs

133396 (Human); 218624 (Mouse)

Alternate Names

class I cytokine receptor; CRL3; CRL3Glmr; Cytokine receptor-like 3; GLMR; GLM-R; GLM-RMGC125346; GP130 like receptor; Gp130-like monocyte receptor; Gp130-like receptor; hGLM-R; IL-31 RA; IL-31 receptor subunit alpha; IL-31R subunit alpha; IL31RA; IL-31RA; IL-31RAGPLCRL; IL-31R-alpha; interleukin 31 receptor A; interleukin-31 receptor subunit alpha; PRO21384; soluble type I cytokine receptor CRL3; zcytoR17