Recombinant Mouse IL-36Ra/IL-1F5 Protein Summary
Product Specifications
Val2-Asp156
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
2714-ML
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS and DTT with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 250 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
2714-ML/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS and DTT. |
| Reconstitution | Reconstitute at 250 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: IL-36Ra/IL-1F5
Mouse interleukin-36 receptor antagonist (IL-36Ra; previously IL-1F5; also named FIL-1δ [delta], IL‑1HY1, IL-1H3, and IL-1L1) is a member of the IL‑1 family of proteins (1-6). IL‑1 family members include IL-1 beta, IL-1 alpha, IL-1ra, IL‑18 and IL‑1F5 through F10 (6, 7). All family members show a 12 beta -strand, beta -trefoil configuration, and are believed to have arisen from a common ancestral gene that underwent multiple duplications (8). The mouse IL‑36Ra/IL‑1F5 gene maps to a region on mouse chromosome 2 that contains all other IL-1 family members (except IL‑18), supporting an evolutionary relationship with the IL-1 family (1, 9). It is particularly close to the gene for IL-1ra and is likely a relatively recent duplication of that gene. IL‑36Ra/IL‑1F5 is synthesized as a 156 amino acid (aa) protein that contains no signal sequence, no prosegment and no potential N-linked glycosylation site(s) (2, 5, 8). Nevertheless, it appears to be secreted as a 17 kDa monomer. In humans, there is an alternate start site that potentially gives rise to an alternate splice form (5). This translated product has a premature stop codon, resulting in a truncated 16 aa peptide. Mouse to human, full length IL‑36Ra/IL‑1F5 has 90% aa identity. Within the family, IL-36Ra/IL-1F5 is 48%, 30%, 35%, 35%, 35%, 37% and 43% aa identical to IL‑1ra, IL‑1 beta, IL‑36 alpha /IL‑1F6, IL‑37/IL‑1F7, IL-36 beta /IL-1F8, IL‑36 gamma /IL‑1F9 and IL‑1F10, respectively. Cells reported to express IL-36Ra/IL-1F5 include monocytes, B cells, dendritic cells/Langerhans cells, keratinocytes, and gastric fundus Parietal and Chief cells (1, 8). The receptor for IL-36Ra/IL-1F5 has not been positively identified. Indirect evidence suggests it is IL‑1 Rrp2 and/or IL-1 RAcP (9). In either case, activity association with receptor binding is unclear. It was initially reported to be an antagonist of IL-36 gamma activity (4, 7). This would be consistent with its hypothesized relationship to IL‑1ra.
- Smith, D. E. et al. (2000) J. Biol. Chem. 275:1169.
- Kumar, S. et al. (2000) J. Biol. Chem. 275:10308.
- Mulero, J.J. et al. (1999) Biochem. Biophys. Res. Commun. 263:702.
- Nicklin, M.J.H. et al. (2002) Genomics. 79:718.
- Barton J. L. et al. (2000) Eur. J. Immunol. 30:3299.
- Dinarello, C. et al. (2010) Nat. Immunol. 11:973.
- Dunn, E. et al. (2001) Trends Immunol. 22:533.
- Debets, R. et al. (2001) J. Immunol. 167:1440.
- Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.
Citations for Recombinant Mouse IL-36Ra/IL-1F5 Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Interleukin-36 Receptor Signaling Attenuates Epithelial Wound Healing in C57BL/6 Mouse Corneas
Authors: Chen, Q;Gao, N;Yu, FS;
Cells
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Interleukin-36 cytokines alter the intestinal microbiome and can protect against obesity and metabolic dysfunction
Authors: E Giannoudak, YE Hernandez-, K Mulfaul, SL Doyle, E Hams, PG Fallon, A Mat, D O'Shea, M Kopf, AE Hogan, PT Walsh
Nat Commun, 2019-09-05;10(1):4003.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Opposing Effects of IL-1Ra and IL-36Ra on Innate Immune Response to Pseudomonas aeruginosa Infection in C57BL/6 Mouse Corneas
Authors: N Gao, R Me, C Dai, B Seyoum, FX Yu
J. Immunol., 2018-06-11;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Application of IL-36 receptor antagonist weakens CCL20 expression and impairs recovery in the late phase of murine acetaminophen-induced liver injury.
Authors: Scheiermann P, Bachmann M, Hardle L, Pleli T, Piiper A, Zwissler B, Pfeilschifter J, Muhl H
Sci Rep, 2015-02-17;5(0):8521.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo
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