Recombinant Mouse IL1RAPL2 Fc Chimera Protein, CF

Catalog #: 11222-MR Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
11222-MR-050

Recombinant Mouse IL1RAPL2 Fc Chimera Protein Binding Activity.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse IL1RAPL2 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse IL1RAPL2 Fc Chimera (Catalog # 11222-MR) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human PTPRD Fc Chimera (Catalog # 9995-PR) binds with an ED₅₀ of 20.0-240 ng/mL.

Source

Mouse myeloma cell line, NS0-derived mouse IL1RAPL2 protein

Mouse IL1RAPL2 (Thr17-Lys354) Accession # Q9ERS6.1	IEGRMDP	Mouse IgG_2A (Glu98-Lys330)
N-terminus		C-terminus

Accession #

Q9ERS6.1

N-terminal Sequence
Analysis

Thr17

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

66 kDa

SDS-PAGE

75-88 kDa, under reducing conditions.

Product Datasheets

11222-MR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11222-MR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Mouse IL1RAPL2 Fc Chimera Protein (Catalog # 11222-MR) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human PTPRD Fc Chimera Protein (9995-PR) binds with an ED₅₀ of 20.0-240 ng/mL.

SDS-PAGE

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2 μg/lane of Recombinant Mouse IL1RAPL2 Fc Chimera Protein (Catalog # 11222-MR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 75-88 kDa.

Reconstitution Calculator

Background: IL1RAPL2

The Interleukin 1 receptor family (IL-1R) comprises at least eleven members including IL-1 RI (IL-1R1), IL-1 RII (IL-1 R2), IL-1 RAcP (IL-1 R3), ST2 (T1/IL‑1 R4), IL‑18 R alpha (IL-1 Rrp/IL-1 R5), IL-1 Rrp2 (IL-1 RL2/IL-1 R6), IL-18 R beta (AcPL/IL-1 R7), IL1RAPL1 (TIGIRR-2/IL-1 R8), and IL1RAPL2 (TIGIRR-1/IL-1 R9) (1). All family members possess three immunoglobulin (Ig)-like domains in their extracellular region. Most members also have an intracellular TIR (Toll-like receptor/IL-1 receptor signaling) domain that is also conserved in the Toll-like receptor family. Related proteins, SIGIRR (single Ig domain-containing IL-1 R-related molecule) and IL-18BP, differ from the other members by having only one Ig domain (1). IL-1 receptor accessory protein-like 2 (IL1RAPL2) is alternately known as IL-1 R9 and three immunoglobulin domain containing IL-1 receptor-related molecule 1 (TIGIRR-1) and is expressed in the brain (2). Human IL1RAPL2 is a type I transmembrane glycoprotein with a 16 aa signal peptide, a 338 aa extracellular region containing three Ig-like domains, an 18 aa transmembrane domain and a 311 aa cytoplasmic tail (3). By comparison to other IL-1 receptor family proteins, IL1RAPL2 has a C-terminal cytoplasmic extension beyond the TIR domain that is found in IL-1RAPL1 and SIGIRR but not other family members (3). Human and mouse IL1RAPL2 share approximately 95% aa sequence identity. Human IL1RAPL2 is most homologous (63%) to IL-1RAPL1, a receptor protein that is highly expressed in hippocampus and is involved in X-linked mental retardation (4, 5). Genes for both have been localized to human chromosome Xq22. A ligand for IL1RAPL2 has not been identified (1).

References

Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
Andre, R. et al. (2005) J. Neurochem. 95:324.
Born, T.L. et al. (2000) J. Biol. Chem. 275:29946.
Jin, H. et al. (2000) Eur. J. Hum. Genet. 8:87.
Carrie, A. et al. (1999) Nat. Genet. 23:25.

Long Name

Interleukin 1 Receptor Accessory Protein-like 2

Entrez Gene IDs

26280 (Human); 60367 (Mouse)

Alternate Names

IL-1R10; IL-1R-9; IL1R9IL-1 receptor accessory protein-like 2; IL-1R9IL-1RAPL-2; IL1RAPL2; IL-1RAPL2; IL-1-RAPL-2; IL1RAPL-2IL1RAPL-2-related protein; interleukin 1 receptor 9; interleukin 1 receptor accessory protein-like 2; Three immunoglobulin domain-containing IL-1 receptor-related 1; TIGIRR-1; TIGIRR-1Interleukin-1 receptor 9; X-linked interleukin-1 receptor accessory protein-like 2