What is the amino acid sequence of the acidic and basic tails?

Name: Recombinant Mouse Integrin alpha 1 beta 1 Protein, CF
Brand: R&D Systems
SKU: 8188-AB
Price: 423 USD

Acidic and basic tails are added to the protein to help facilitate optimal activity. While we generally include sequence information on the product datasheet, the sequences of these tails are considered confidential information.

Recombinant Mouse Integrin alpha 1 beta 1 Protein, CF

Catalog #: 8188-AB
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
8188-AB-025

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Citations (3)

FAQs

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Integrin alpha 1 beta 1 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE with silver staining

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Collagen I is immobilized at 5 μg/mL, Recombinant Mouse Integrin alpha 1 beta 1 can bind with an apparent K_d <5 nM.
Optimal dilutions should be determined by each laboratory for each application.

Source

Chinese Hamster Ovary cell line, CHO-derived mouse Integrin alpha 1 beta 1 protein

Mouse Integrin alpha 1 (Phe29-Pro1141) Accession # Q3V3R4	His-Pro	GGGSGGGS	Acidic Tail	6-His tag
Mouse Integrin beta 1 (Gln21-Asp728) Accession # P09055	His-Pro	GGGSGGGS	Basic Tail
N-terminus				C-terminus

Accession #

Q3V3R4(Integrin alpha 1) & P09055 (Integrin beta 1)

N-terminal Sequence
Analysis

Phe29 (Integrin alpha 1) & No results obtained: Gln21 predicted (Integrin beta 1), sequencing might be blocked

Structure / Form

Noncovalently-linked heterodimer

Predicted Molecular Mass

132 kDa (Integrin alpha 1) & 86.5 kDa (Integrin beta 1)

SDS-PAGE

120-135 kDa & 180-215 kDa, reducing conditions

Product Datasheets

8188-AB

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8188-AB

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Integrin alpha 1 beta 1

Integrin alpha 1 beta 1, also called VLA1, is the only alpha 1 integrin family adhesion receptor, one of twelve integrins that share the beta 1 subunit, and one of four collagen-binding integrins (1-6). It is the non-covalent heterodimer of 190-210 kDa alpha 1 (CD49a) and 130 kDa beta 1 (CD29) type I transmembrane glycoprotein subunits. It is found on cells including activated T cells, B cells, monocytes, vascular smooth muscle cells, osteoblasts and adipocytes (2, 7-9). The alpha 1 extracellular domain (ECD) contains an I (inserted) domain which includes the ligand binding site (2, 4). The beta 1 ECD contains a vWFA domain, which participates in binding (3). Each subunit then has a transmembrane sequence and a short cytoplasmic tail. Divalent cations and intracellular (inside-out) signaling convert the dimer from the folded, inactive form to its most active, extended conformation (1, 2). The 1113 amino acid (aa) mouse alpha 1 extracellular domain (ECD) shares 88% and 96% aa sequence identity with human and rat alpha 1, respectively, while the 708 aa mouse beta 1 ECD shares 93% and 98% aa sequence identity with human and rat beta 1, respectively. alpha 1 beta 1 preferentially binds collagens I, IV, VI, XIII and XVI, but also binds laminin (4-11). alpha 1 beta 1 is reported to down-regulate EGF R signaling, increase expression of caveolin-1, reduce production of reactive oxygen species, regulate collagen expression, control MMP collagenase and gelatinase activity, and mediate the renal basement membrane disorder Alport syndrome (11-13). These effects may begin by alpha 1 beta 1 binding of caveolin-1, initiating signaling pathways that involve the phosphatase TC-PTP, kinases ERK and p38, and the transcription factor PPAR-gamma (11-14). alpha 1 beta 1 down-regulates MMP-mediated angiostatin formation, enhancing tumor vascularization (9). alpha 1 beta 1-null mice are deficient in fibroblast collagen IV and laminin-mediated cell spreading and migration, show defects in bone healing, and are resistant to Alport renal fibrosis (10-12, 15). When expressed in the same epithelial cells, alpha 1 beta 1 negatively regulates integrin alpha 2 beta 1-mediated cell adhesion and migration (16).

References

Takada, Y. et al. (2007) Genome Biol. 8:215.
Luo, B-H. et al. (2007) Annu. Rev. Immunol. 25:619.
Argraves, W.S. et al. (1987) J. Cell Biol. 105:1183.
Briesewitz, R. et al. (1993) J. Biol. Chem. 268:2989.
Holers, V.M. et al. (1989) J. Exp. Med. 169:1589.
Tulla, M. et al. (2001) J. Biol. Chem. 276:48206.
Eble, J.A. et al. (2006) J. Biol. Chem. 281:25745.
Hall, D.E. et al. (1990) J. Cell Biol. 110:2175.
Pozzi, A. et al. (2000) Proc. Natl. Acad. Sci. USA 97:2202.
Gardner, H. et al. (1999) J. Cell Sci. 112:263.
Dennis, J. et al. (2010) Am. J. Pathol. 177:2527.
Cosgrove, D. et al. (2008) Am. J. Pathol. 172:761.
Chen, X. et al. (2010) Mol. Cell. Biol. 30:3048.
Borza, C.M. et al. (2010) J. Biol. Chem. 285:40114.
Gardner, H. et al. (1996) Dev. Biol. 175:301.
Abair, T.D. et al. (2008) Exp. Cell Res. 314:3593.

Entrez Gene IDs

3672 (Human)

Alternate Names

Integrin alpha 1 beta 1

Related Research Areas

Citations for Recombinant Mouse Integrin alpha 1 beta 1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Chronic encephalomyelitis virus exhibits cellular tropism and evades pDCs by binding to sialylated integrins as the cell surface receptors
Authors: Takeda, K;Kaifu, T;Michihata, R;Kinugawa, N;Fujioka, A;Tateno, A;Toshima, K;Kanoh, H;Inamori, KI;Kamijo, K;Himeda, T;Ohara, Y;Inokuchi, JI;Nakamura, A;
European journal of immunology
Species: Mouse
Sample Types: Recombinant Protein
Applications: Bioassay
Group B streptococcus exploits vaginal epithelial exfoliation for ascending infection
Authors: J Vornhagen, B Armistead, V Santana-Uf, C Gendrin, S Merillat, M Coleman, P Quach, E Boldenow, V Alishetti, C Leonhard-M, LY Ngo, C Whidbey, KS Doran, C Curtis, KMA Waldorf, E Nance, L Rajagopal
J. Clin. Invest., 2018-04-09;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo
Proteolytic Isoforms of SPARC Induce Adipose Stromal Cell Mobilization in Obesity.
Authors: Tseng C, Kolonin M
Stem Cells, 2015-09-22;34(1):174-90.
Applications: Bioassay

FAQs

What is the amino acid sequence of the acidic and basic tails?
- Acidic and basic tails are added to the protein to help facilitate optimal activity. While we generally include sequence information on the product datasheet, the sequences of these tails are considered confidential information.

View all Proteins and Enzyme FAQs