Recombinant Mouse Lefty-1 Protein

Catalog #: 994-LF Datasheet / COA / SDS

Carrier Free

Catalog #	Availability	Size / Price	Qty
994-LF-025/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
994-LF-025

R&D Systems Recombinant Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Lefty-1 Protein Summary

Product Specifications

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to induce cell death using Mv1Lu mink lung epithelial cells. The ED₅₀ for this effect is 0.5-3 μg/mL.

Source

Mouse myeloma cell line, NS0-derived mouse Lefty-1 protein

Mouse Lefty-1 Proregion (Leu22-Arg135) Accession # NP_034224	6-His tag	Mouse Lefty mature (Leu136-Pro368) Accession # NP_034224
	6-His tag	Mouse Lefty mature (Leu136-Pro368) Accession # NP_034224
N-terminus		C-terminus

A short DNA sequence encoding 6 histidine residues was inserted into the DNA sequence encoding mouse Lefty-1 between Arg135 and Leu136. Two peptides corresponding to mouse Lefty-1 propeptide and mature mouse Lefty-1 are present in the recombinant preparation.

Accession #

NP_034224

N-terminal Sequence
Analysis

Leu22 (pro) & His (mature)

Predicted Molecular Mass

26.9 kDa

SDS-PAGE

12 kDa and 36 kDa, reducing conditions

Product Datasheets

994-LF (with carrier)

Product Datasheet

COA

SDS

994-LF/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

994-LF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 0.5-2 mg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

994-LF/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 0.5-2 mg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Lefty-1

Lefty was first identified in a screen for undifferentiated cell-specific cDNAs from the P19 mouse embryonal carcinoma cells. Its mRNA expression on the left side of the developing embryo earned it the name “lefty”. Two genes exist in mouse (Lefty-1 and Lefty-2) and two in humans (Lefty-A (ebaf) and Lefty-B). By amino acid sequence, mouse Lefty-1 and -2 are more similar to each other (90%) than to either Lefty-A or -B in humans (81 - 82% identical). Lefty contains the six cysteine residues that are conserved among TGF-beta related proteins and that are necessary to form the cysteine-knot structure. However, lefty is distinct from other family members in that it has two RXXR cleavage sites, a longer carboxy terminal sequence, and it lacks the cysteine residue required for intermolecular disulfide linkage. Thus, mature forms of lefty are larger than mature forms of other TGF-beta -related proteins. Mouse Lefty-1 is differentially processed depending on the cells in which it is synthesized, and both processing sites can be utilized.

Lefty homologues have been identified in other vertebrate organisms including chick, frog, and zebrafish. Although the amino acid sequence identity is not well conserved among vertebrate species, the expression pattern of lefty on the left side is well conserved. Lefty-1 is expressed strongly on the left side of the prospective floor plate and weakly in the left half of the lateral plate mesoderm in E8 mice embryos. In all species examined, lefty proteins function in patterning left-right asymmetry of the developing organ systems such as the heart and lung. Lefty acts as an antagonist to Nodal signaling, potentially by competing for binding to a common receptor.

References