Recombinant Mouse Lgr6 Fc Chimera Protein, CF

Catalog #: 8029-GP Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
8029-GP-050

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Lgr6 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE with silver staining, under reducing conditions

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse R‑Spondin 3 (Catalog # 4120-RS) is coated at 0.5 μg/mL, Recombinant Mouse Lgr6 Fc Chimera binds with an apparent K_d <1.0 nM.

Source

Chinese Hamster Ovary cell line, CHO-derived mouse Lgr6 protein

Mouse Lgr6 (Gly23-Ala567) Accession # Q3UVD5	IEGRMDP	Mouse IgG_2A (Glu98-Lys330)
N-terminus		C-terminus

Accession #

Q3uvd5

N-terminal Sequence
Analysis

Gly23

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

87.9 kDa (monomer)

SDS-PAGE

95-105 kDa, reducing conditions

Product Datasheets

8029-GP

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8029-GP

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Lgr6

Lgr6 (leucine‑rich repeat G‑protein-coupled receptor 6) is a seven‑transmembrane glycoprotein receptor in the Lgr family of cell surface receptors (1). While this family includes receptors for hormones such as LH, FSH, TSH, and HCG, the subfamily comprising Lgr4, Lgr5, and Lgr6 are G‑protein‑independent mediators of the potentiating effect of R‑Spondins on Wnt signaling (1‑3). Each has been identified as a marker for specific types of stem cells in tissues that are subject to constant self‑renewal such as skin, gastrointestinal tract and lungs. Mouse Lgr6 cDNA encodes 967 amino acids (aa), including a signal sequence (aa 1‑22), a long N‑terminal extracellular domain (ECD) with 15‑16 LRR domains that mediate ligand interaction (aa 25‑567), a seven‑transmembrane region (aa 568‑830), and a C‑terminal cytoplasmic region (aa 831‑967) (1). The N‑terminal ECD of mouse Lgr6 shares 92%, 97%, 91%, 88%, 83% and 77% aa sequence identity with human, rat, canine, feline, porcine and bovine Lgr6, respectively, and 55‑65% aa identity with mouse Lgr4 and Lgr5. Lgr6 expression is detected in rare cells of the brain, mammary gland, lung and skin, and is expressed on stem cells above the hair follicle bulge in the skin (2). Lgr6⁺ stem cells can produce sebaceous gland or interfollicular epidermis as well as hair follicles in vivo, during prenatal and postnatal differentiation and wound healing (2). In vitro, they can produce multiple mesenchymal lineages or neurons (4). Like Lgr4 and Lgr5, Lgr6 can bind and respond to R‑Spondins, and can enhance Wnt/ beta ‑catenin signaling through increasing phosphorylation of LRP Wnt co‑receptors (3, 5). Lgr6 is often up‑regulated in human gastric cancers as compared to the surrounding tissues (6).

References

Hsu, S.Y. et al. (1998) Mol. Endocrinol. 12:1830.
Snippert, H.J. et al. (2010) Science 327:1385.
Gong, X. et al. (2012) PLoS ONE 7:e37137.
Nath, M. et al. (2011) Cell Tissue Res. 344:435.
de Lau, W. et al. (2011) Nature 476:293.
Steffen, J.S. et al. (2012) Virchows Arch. 461:355.

Long Name

Leucine-rich Repeat Containing G Protein-coupled Receptor 6

Entrez Gene IDs

59352 (Human); 329252 (Mouse); 498233 (Rat)

Alternate Names

FLJ14471; gonadotropin receptor; GPCR; leucine-rich repeat containing G protein-coupled receptor 6; leucine-rich repeat-containing G protein-coupled receptor 6; leucine-rich repeat-containing G-protein coupled receptor 6; Lgr6; VTS20631