Recombinant Mouse Limitin/IFN-zeta Fc Chimera Protein, CF

Catalog #: 597-LM Datasheet / COA / SDS

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597-LM-010

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Limitin/IFN-zeta Fc Chimera Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Activity

Measured in an anti-viral assay using L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. Vogel, S.N. et al. (1982) Infect. Immunol. 38:681. The ED₅₀ for this effect is 0.1-0.5 ng/mL.

Source

Mouse myeloma cell line, NS0-derived mouse Limitin/IFN-zeta protein

Mouse Limitin (Leu22 - Arg182) Accession # BAA83749	IEGRMD	Human IgG₁ (Pro100 - Lys330)
N-terminus		C-terminus

Accession #

BAA83749

N-terminal Sequence
Analysis

Leu22

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

45.0 kDa (monomer)

SDS-PAGE

50-55 kDa, reducing conditions

Product Datasheets

597-LM

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

597-LM

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Limitin/IFN-zeta

Limitin is a secreted interferon (IFN)-like glycoprotein. It has antiviral activity and selectively inhibits B-lineage lymphoid-cell development both in vivo and in vitro, while having little effect on myelopoiesis or erythropoiesis. Mouse Limitin cDNA encodes a 182 amino acid (aa) residue precursor protein with an N-terminal 21 aa residue putative signal peptide. Limitin binds to the IFN-alpha / beta receptors and induces IFN regulatory factor-1. Mouse Limitin displays 32%, 26% and 30% amino acid identity with mouse IFN-alpha, mouse IFN-beta, and human IFN-omega, respectively.

References