Recombinant Mouse LRP-6 C-Terminal Fragment Fc Protein, CF

Catalog #: 9954-LR Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9954-LR-050

Recombinant Mouse LRP-6 C-Terminal Fragment Fc Protein Binding Activity

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Product Details

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse LRP-6 C-Terminal Fragment Fc Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. In a Goat Anti-Mouse IgG Fc Antibody (Catalog # G-202-C) coated plate, Recombinant Mouse LRP‑6 C-Terminal Fragment Fc Chimera binds Recombinant Mouse Dkk-1 (Catalog # 5897-DK) with an ED₅₀ of 3-30 ng/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived mouse LRP-6 protein

Mouse LRP-6 (Val629-Gly1244) Accession # NP_032540	IEGRMDP	Mouse IgG_2a (Glu98-Lys330)
N-terminus		C-terminus

Accession #

NP_032540

N-terminal Sequence
Analysis

Val629

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

97 kDa

SDS-PAGE

97-118 kDa, reducing conditions

Product Datasheets

9954-LR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9954-LR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	12 months from date of receipt, ≤ -20 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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In a Goat Anti-Mouse IgG Fc (Catalog # G-202-C) coated plate, Recombinant Mouse LRP-6 C-Terminal Fragment Fc Chimera (Catalog # 9954-LR) binds Recombinant Mouse DKK-1 (Catalog # 5897-DK) with an ED₅₀ of 3-30 ng/mL.

SDS-PAGE

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2 μg/lane of Recombinant Mouse LRP‑6 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 97-118 kDa and 200-230 kDa, respectively.

Reconstitution Calculator

Background: LRP-6

The low-density lipoprotein (LDL) receptor-related protein 6 (LRP-6), along with LRP-5, constitute a distinct subgroup of the LDL receptor family (1). Both LRP-5 and LRP-6 are type I transmembrane proteins that function as Wnt co-receptors with Frizzled proteins and mediate the down-regulation of GSK-3 activity and the initiation of the canonical Wnt/beta-catenin signaling cascade (1, 2). Mouse LRP-6 consists of a 19 amino acid (aa) signal sequence, a 1351 aa extracellular domain (ECD), a 23 aa transmembrane domain, and a 20 aa cytoplasmic domain (3). The LRP-6 ECD is composed of four distinct YWTD beta -propeller (BP) motifs followed by three LDLR type A repeats (4). The first two LRP-6 BP motifs bind Wnt-1, -2, -2b, -6, -8a, -9a, -9b, and -10b, while the third and fourth BP motifs bind Wnt-3a (5-10). This product includes the third and fourth BP motifs of mouse LRP-6 (aa 629-1244) that share 98% aa sequence identity with the third and fourth BP motifs of rat and human LRP-6. Dkk-1, a known antagonist of Wnt signaling, interacts with LRP-6 as a bipartite inhibitor, binding the first and third BP motifs of LRP-6 simultaneously (8, 11). Mice lacking LRP-6 die at birth with a truncation of the axial skeleton, limb defects, microphthalmia, and malformation of the urogenital system (2). Additionally, LRP-6 may be essential for osteoblast differentiation during bone remodeling and, along with LRP-5, is required for postnatal bone development (12, 13).

References