Recombinant Mouse MARCO Protein, CF

Catalog #: 2956-MA
1 Citation Datasheet / COA / SDS

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2956-MA-050

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse MARCO Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to bind fluorescein-conjugated E. coli Bioparticles. Sankala, M. et al. (2002) J. Biol. Chem. 277:33378. The ED₅₀ for this effect is 0.5-2.5 µg/mL.

Source

Mouse myeloma cell line, NS0-derived mouse MARCO protein
Gln70-Ser518, with an N-terminal 9-His tag

Accession #

Q60754

N-terminal Sequence
Analysis

His

Predicted Molecular Mass

46.3 kDa

SDS-PAGE

57-60 kDa, reducing conditions

Product Datasheets

2956-MA

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2956-MA

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: MARCO

MARCO (macrophage receptor with collagenous structure), also known as SCARA2, is an 80 kDa type II transmembrane glycoprotein that belongs to the class A scavenger receptor family (1). Mouse MARCO consists of a 48 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 449 aa extracellular domain (ECD) that includes a stalk region, a collagen-like region, and one SRCR domain (2). Within the ECD, mouse MARCO shares 69% and 86% aa sequence identity with human and rat MARCO, respectively. It shares 18%-28% aa sequence identity with other mouse class A scavenger receptors CL-P1, SCARA3, SCARA5, and SR-A1/MSR. MARCO is constitutively expressed on the surface of splenic and lymph node macrophages (2, 3). Its expression is induced on Kupffer cells and alveolar macrophages by microbial infection, chemical irritants, and Th1 polarizing factors (3-5). MARCO binds LPS, lipoteichoic acid, and other determinants on Gram positive and Gram negative bacteria (2, 6-8). It also binds modified LDL, CpG oligonucleotides, UGRP1, silica, and TiO₂ (2, 9-11). MARCO is required for the organization of the splenic marginal zone and the interaction of local macrophages and B cells (12, 13). The SRCR domain mediates binding of MARCO to its various ligands (3, 12), while the collagen-like region mediates assembly into a disulfide-linked trimeric molecule (2, 7). MARCO ligation induces, but is not required for the production of IL-12, NO, or TNF-alpha by macrophages (5, 6, 9). MARCO knockout mice show a reduced clearance of bacterial infections, reduced mast cell mediated silicosis, increased pulmonary inflammation, and increased sensitivity to ozone induced lung damage (4, 9, 14-16).

References

Murphy, J.E. et al. (2005) Atherosclerosis 182:1.
Elomaa, O. et al. (1995) Cell 80:603.
Van der Laan, L.J.W. et al. (1999) J. Immunol. 162:939.
Dahl, M. et al. (2007) J. Clin. Invest. 117:757.
Jozefowski, S. et al. (2005) J. Immunol. 175:8032.
Mukhopadhyay, S. et al. (2006) Eur. J. Immunol. 36:940.
Sankala, M. et al. (2002) J. Biol. Chem. 277:33378.
Chen, Y. et al. (2006) J. Biol. Chem. 281:12767.
Jozefowski, S. et al. (2006) J. Leukoc. Biol. 80:870.
Bin, L.-H. et al. (2003) J. Immunol. 171:924.
Hamilton, Jr. R.F. et al. (2006) J. Biol. Chem. 281:34218.
Karlsson, M.C.I. et al. (2003) J. Exp. Med. 198:333.
Chen, Y. et al. (2005) J. Immunol. 175:8173.
Arredouani, M. et al. (2004) J. Exp. Med. 200:267.
Arredouani, M.S. et al. (2007) J. Immunol. 178:5912.
Brown, J.M. et al. (2007) Am. J. Respir. Cell Mol. Biol. 36:43.

Long Name

Macrophage Receptor with Collagenous Structure/Scavenger Receptor, Class A, Member 2

Entrez Gene IDs

8685 (Human); 17167 (Mouse)

Alternate Names

macrophage receptor with collagenous structureSCARA2Scavenger receptor class A member 2; MARCO; member 2; SCARA2

Related Research Areas

Citation for Recombinant Mouse MARCO Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1