Recombinant Mouse MBL-2 Protein

Name: Recombinant Mouse MBL-2 Protein
Brand: R&D Systems
SKU: 2208-MB
Rating: 4 (1 reviews)

Catalog #: 2208-MB
3 Citations 1 Review Datasheet / COA / SDS

Carrier Free

Catalog #	Availability	Size / Price	Qty
2208-MB-050/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
2208-MB-050

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Product Details

Citations (3)

FAQs

Reviews (1)

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse MBL-2 Protein Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to bind with Mannan in a functional ELISA.

Source

Mouse myeloma cell line, NS0-derived mouse MBL-2 protein
Glu19-Asp244

Accession #

Q3UEK1

N-terminal Sequence
Analysis

Glu19

Structure / Form

Oligomer

Predicted Molecular Mass

24 kDa (monomer)

SDS-PAGE

30 kDa, reducing conditions

Product Datasheets

2208-MB (with carrier)

Product Datasheet

COA

SDS

2208-MB/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2208-MB

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

2208-MB/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: MBL-2

Mannan binding lectin (MBL) belongs to the collectin family of innate immune defense proteins, which binds to an array of carbohydrate patterns on pathogen surfaces (1, 2). Collectin family members share common structural features: a cysteine rich amino-terminal domain, a collagen-like region, an alpha -helical coiled-coil neck domain and a carboxy terminal C-type (Ca⁺⁺-dependent) lectin or carbohydrate recognition domain (CRD). MBL homotrimerizes to form a structural unit joined byN-terminal disulfide bridges. These homotrimers further associates into oligomeric structures of up to 6 units. Whereas two forms of MBL proteins (MBL-1, also known as S-MBP or MBL-A and MBL-2, also known as L-MBP or MBL-C) exist in rodents and other animals, only one functional MBL protein is present in humans. Mouse MBL-2 shares about 52% and 60% amino acid sequence identity with mouse MBL-1 and human MBL, respectively.

In mouse, MBL-1 and MBL-2 are the only collectins that can activate complement via the lectin complement pathway (1, 2). Serum oligomeric MBL associates with MBL-associated serine protease (MASP) proenzymes. The MBL-MASP proenzyme complex preferentially interact with sugar patterns containing mannose, glucose, L-fucose, or N-acetyl-glucosamine present at a terminal nonreducing postion on the cell surface of various pathogens and certain tumor cells. This interaction induces pro-enzyme activation and the triggering of the complement cascade, resulting in opsonization and pathogen removal via humoral and cellular immune responses. MBL does not recognize self-components or glycoproteins from other higher animals due to the presence of terminal sialic acid or galactose that interrupts the repeating carbohydrate structures (3). A number of membrane receptors for MBL, including C1q phagocytic receptor (C1qRp), calreticulin (also known as C1qR), and CR1 (CD35), have been described. Interactions with these receptors may also be important in stimulating phagocytosis (1, 2).

Mouse MBL-1 and MBL-2 are produced primarily in the liver and are secreted into the blood stream. In addition, mouse MBL-1 is also expressed in lung, kidney and testis while MBL-2 is expressed in kidney, thymus and small intestine (1, 4, 5).

References

Holmskov, U. et al. (2003) Annu. Rev. Immunol. 21:547.
Fujita, R. et al. (2004) Immunol. Rev. 198:185.
Saevarsdottir, S. et al. (2004) Scand. J. Immnunol. 60:23.
Uemura, K. et al. (2002) J. Immunol. 169:6945.
Wagner, S. et al. (2003) J. Immunol. 170:1462.

Long Name

Mannose Binding Lectin 2

Entrez Gene IDs

17195 (Mouse)

Alternate Names

L-MBP; mannose-binding protein C; L-MBP; mannose-binding lectin (protein C) 2; MBL; MBL-2; MBL-C; MBP-C

Related Research Areas

Citations for Recombinant Mouse MBL-2 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Interleukin-17 receptor A (IL-17RA) as a central regulator of the protective immune response against Giardia
Authors: O Paerewijck, B Maertens, L Dreesen, F Van Meulde, I Peelaers, D Ratman, RW Li, E Lubberts, K De Bossche, P Geldhof
Sci Rep, 2017-08-17;7(1):8520.
Species: Parasite - Giardia duodenalis
Sample Types: Trophozoites
Applications: Bioassay
Role of pentraxin 3 in shaping arthritogenic alphaviral disease: from enhanced viral replication to immunomodulation.
Authors: Foo, Suan-Sin, Chen, Weiqiang, Taylor, Adam, Sheng, Kuo-Chin, Yu, Xing, Teng, Terk-Shi, Reading, Patrick, Blanchard, Helen, Garlanda, Cecilia, Mantovani, Alberto, Ng, Lisa F P, Herrero, Lara J, Mahalingam, Suresh
PLoS Pathog, 2015-02-19;11(2):e1004649.
Species: Mouse
Sample Types: Virus
Applications: Bioassay
Mice deficient in ficolin, a lectin complement pathway recognition molecule, are susceptible to Streptococcus pneumoniae infection.
Authors: Endo, Yuichi, Takahashi, Minoru, Iwaki, Daisuke, Ishida, Yumi, Nakazawa, Naomi, Kodama, Toshihis, Matsuzaka, Tomohiro, Kanno, Kazuko, Liu, Yu, Tsuchiya, Kohsuke, Kawamura, Ikuo, Ikawa, Masahito, Waguri, Satoshi, Wada, Ikuo, Matsushita, Misao, Schwaeble, Wilhelm, Fujita, Teizo
J Immunol, 2012-11-12;189(12):5860-6.
Species: Bacteria - Streptococcus pneumoniae
Sample Types: Whole Cells
Applications: Binding Assay