Recombinant Mouse Neuroplastin Protein, CF Summary
Product Specifications
Gln29-Pro338, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
7818-NP
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 250 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Neuroplastin
Neuroplastin (Np65), also known as gp65 and SDR1, is a widely expressed and variably glycosylated transmembrane member of the CD147/Basigin protein family (1, 2). The 62-75 kDa mature mouse Neuroplastin consists of a 310 amino acid (aa) extracellular region (ECD) with three immunoglobulin-like domains, a 21 aa transmembrane segment, and a 38 aa cytoplasmic domain (3, 4). Within the ECD, mouse Neuroplastin shares 94% and 98% aa sequence identity with human and rat Neuroplastin, respectively. Alternative splicing of mouse Neuroplastin generates additional isoforms that lack the first Ig-like domain, most of the first two Ig-like domains, and/or carry a 9 aa substitution in the cytoplasmic domain. The isoform lacking the first Ig-like domain is known as Neuroplastin-55. Neuroplastin is expressed on cerebellar Purkinje cells and granule cells, hippocampal pyramidal neurons, and retinal photoreceptors (5 - 9). It serves as a homophilic cell adhesion molecule on neurons and promotes neurite outgrowth (7, 8). The neurite outgrowth function requires its binding to and activation of FGFR1 (7, 10). In the synapse, Neuroplastin binds to multiple subunits of the GABA-A receptor, reduces synaptic expression of GluR1, and contributes to synaptic plasticity (7, 8, 11, 12). Neuroplastin overexpression can enhance tumor growth, VEGF production, and vascularization (13).
- Owczarek, S. and V. Berezin (2012) Int. J. Biochem. Cell Biol. 44:1.
- Langnaese, K. et al. (1998) FEBS Lett. 429:284.
- Shirozu, M. et al. (1996) Genomics 37:273.
- Langnaese, K. et al. (1997) J. Biol. Chem. 272:821.
- Marzban, H. et al. (2003) J. Comp. Neurol. 462:286.
- Bernstein, H-G. et al. (2007) Brain Res. 1134:107.
- Owczarek, S. et al. (2011) J. Neurochem. 117:984.
- Smalla, K-H. et al. (2000) Proc. Natl. Acad. Sci. USA 97:4327.
- Kreutz, M.R. et al. (2001) Invest. Ophthalmol. Vis. Sci. 42:1907.
- Owczarek, S. et al. (2010) FASEB J. 24:1139.
- Sarto-Jackson, I. et al. (2012) J. Biol. Chem. 287:14201.
- Empson, R.M. et al. (2006) J. Neurochem. 99:850.
- Rodriguez-Pinto, D. et al. (2009) Cancer Immunol. Immunother. 58:221.
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