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Recombinant Mouse Nodal Protein

Catalog #: 1315-ND
12 Citations Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
1315-ND-025/CF

With Carrier

Catalog # Availability Size / Price Qty
1315-ND-025
R&D Systems Recombinant Proteins and Enzymes
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Citations (12)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Nodal Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce Smad2 phosphorylation in P19 mouse embryonal carcinoma cells. Yeo, C. et al. (2001) Mol. Cell. 7:949. Approximately 100 ng/mL of Recombinant Mouse Nodal can effictively induce Smad2 phosphorylation.
Source
E. coli-derived mouse Nodal protein
His245-Leu354, with an N-terminal Met
Accession #
P43021
N-terminal Sequence
Analysis
Met
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
13 kDa (monomer)

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1315-ND (with carrier)

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1315-ND/CF (carrier free)

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COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1315-ND

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 50 μg/mL in sterile 1 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1315-ND/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Nodal

Nodal is a secreted protein that is a member of the Transforming Growth Factor-beta (TGF-beta ) superfamily. Nodal was named for its localized expression in the mouse node during gastrulation, and is first detected in the early primitive streak during mesoderm formation. Expression of the Nodal gene occurs asymmetrically in the left, but not right, lateral plate during somitogenesis. Nodal proteins play crucial roles in mesoderm formation and both anterior-posterior and left-right axis formation during vertebrate development. Members of the Nodal gene family include mouse Nodal, chick cNR-1, frog Xnr1-4, and zebrafish cyclops. Biologically active Nodal is a disulfide-linked homodimer and contains all seven of the cysteine residues necessary for formation of the “cysteine knot” characteristic of TGF-beta -related molecules. Mouse Nodal is 34 - 39% homologous in the conserved region to other TGF-beta superfamily members. Nodal has been shown to signal through a mechanism related to the Activin pathway, and signaling is mediated through both Smad2 and 3. Nodal signaling utilizes type II Activin receptors, together with ALK4/ActRIB, or the orphan type I receptor ALK7. Nodal interacts extracellularly with members of other protein families, including Cerberus, Lefty, and EGF-CFC ligands, such as Cripto. While the Cerberus and Lefty families act as Nodal antagonists, the EGF-CFC molecules act as co-receptors to facilitate Nodal signaling. The resulting concert of regulated Nodal activity allows for the precise control of mesoderm formation, neural patterning, and axis positioning and patterning during early vertebrate development.

References
  1. Kumar, A. et al. (2001) J. Biol. Chem. 276:656.
  2. Reissmann, E. et al. (2001) Genes & Dev. 15: 2010.
  3. Schier, A. and M. Shen (1999) Nature 403:385.
  4. Zhou, X. et al. (1993) Nature 361:543.
Entrez Gene IDs
4838 (Human); 18119 (Mouse)
Alternate Names
BMP-16; MGC138230; nodal homolog (mouse); nodal homolog; Nodal; nodal, mouse, homolog

Citations for Recombinant Mouse Nodal Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Lineage commitment of embryonic cells involves MEK1-dependent clearance of pluripotency regulator Ventx2
    Authors: P Scerbo, L Marchal, L Kodjabachi
    Elife, 2017-06-27;6(0):.
    Species: Xenopus
    Sample Types: In Vivo
    Applications: In Vivo
  2. Response to Nodal morphogen gradient is determined by the kinetics of target gene induction.
    Authors: Dubrulle, Julien, Jordan, Benjamin, Akhmetova, Laila, Farrell, Jeffrey, Kim, Seok-Hyu, Solnica-Krezel, Lilianna, Schier, Alexande
    Elife, 2015-04-14;4(0):.
    Species: Zebrafish
    Sample Types: In Vivo
    Applications: In Vivo
  3. Signaling mechanisms that suppress the cytostatic actions of rapamycin.
    Authors: Jahn S, Law M, Corsino P, Davis B, Harrison J, Law B
    PLoS ONE, 2014-06-13;9(6):e99927.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Rab23 regulates Nodal signaling in vertebrate left-right patterning independently of the Hedgehog pathway.
    Authors: Fuller K, O'Connell J, Gordon J, Mauti O, Eggenschwiler J
    Dev Biol, 2014-04-26;391(2):182-95.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  5. An essential role for maternal control of Nodal signaling.
    Authors: Kumari, Pooja, Gilligan, Patrick, Lim, Shimin, Tran, Long Duc, Winkler, Sylke, Philp, Robin, Sampath, Karuna
    Elife, 2013-09-10;2(0):e00683.
    Species: Zebrafish
    Sample Types: In Vivo
    Applications: In Vivo
  6. Nodal signaling regulates the entry into meiosis in fetal germ cells.
    Authors: Souquet B, Tourpin S, Messiaen S, Moison D, Habert R, Livera G
    Endocrinology, 2012-03-06;153(5):2466-73.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  7. A late requirement for Wnt and FGF signaling during activin-induced formation of foregut endoderm from mouse embryonic stem cells.
    Authors: Hansson M, Olesen DR, Peterslund JM, Engberg N, Kahn M, Winzi M, Klein T, Maddox-Hyttel P, Serup P
    Dev. Biol., 2009-04-07;330(2):286-304.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Small molecules efficiently direct endodermal differentiation of mouse and human embryonic stem cells.
    Authors: Borowiak M, Maehr R, Chen S, Chen AE, Tang W, Fox JL, Schreiber SL, Melton DA
    Cell Stem Cell, 2009-04-03;4(4):348-58.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  9. GDF3 is a BMP inhibitor that can activate Nodal signaling only at very high doses.
    Authors: Levine AJ, Levine ZJ, Brivanlou AH
    Dev. Biol., 2008-09-18;325(1):43-8.
    Species: Xenopus
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Sulfated glycosaminoglycans are necessary for Nodal signal transmission from the node to the left lateral plate in the mouse embryo.
    Authors: Oki S, Hashimoto R, Okui Y, Shen MM, Mekada E, Otani H, Saijoh Y, Hamada H
    Development, 2007-10-03;134(21):3893-904.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  11. Canonical Wnt signaling is required for development of embryonic stem cell-derived mesoderm.
    Authors: Lindsley RC, Gill JG, Kyba M, Murphy TL, Murphy KM
    Development, 2006-08-30;133(19):3787-96.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Role and regulation of nodal/activin receptor-like kinase 7 signaling pathway in the control of ovarian follicular atresia.
    Authors: Wang H, Jiang JY, Zhu C, Peng C, Tsang BK
    Mol. Endocrinol., 2006-05-18;20(10):2469-82.
    Species: Rat
    Sample Types: N/A, Whole Cells
    Applications: Antibody Absorption, Bioassay

FAQs

  1. What is the half-life of this BMP protein?

    • R&D Systems has not determined the half-life of this recombinant human BMP protein. Assay conditions for each protein will need to be optimized by each researcher.

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