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Recombinant Mouse Noggin Fc Chimera Protein, CF

Catalog #: 719-NG
20 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
719-NG-050
719-NG-010
719-NG-100
Recombinant Mouse Noggin Fc Chimera Protein Bioactivity
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Product Details
Citations (20)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Noggin Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.06-0.3 µg/mL in the presence of 30 ng/mL of Recombinant Human BMP-4 (Catalog # 314-BP).
Source
Mouse myeloma cell line, NS0-derived mouse Noggin protein
Mouse Noggin
(Leu20-Cys232)
Accession # U79163		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
U79163
N-terminal Sequence
Analysis
Leu20
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
50 kDa (monomer)
SDS-PAGE
55-60 kDa, reducing conditions

Product Datasheets

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719-NG

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

719-NG

Formulation Lyophilized from a 0.2 μm filtered solution in PBS and EDTA.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Mouse Noggin Fc Chimera Protein Bioactivity View Larger

Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.06-0.3 µg/mL in the presence of 30 ng/mL of Recombinant Human BMP-4 (314-BP).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Noggin

Noggin was originally cloned based on its dorsalizing activity in Xenopus embryos. Mammalian noggins were subsequently identified and cloned from human, mouse and rat cDNA libraries. Mouse noggin cDNA encodes a 232 amino acid (aa) residue precursor protein with 19 aa residue putative signal peptide that is cleaved to generate the 213 aa residue mature protein which is secreted as a homodimeric glycoprotein. Noggin is a highly conserved molecule. Mature mouse noggin shares 99% and 83% aa sequence identity with human and Xenopus noggin, respectively. Noggin has a complex pattern of expression during embryogenesis. In the adult, noggin is expressed in the central nervous system and in several adult peripheral tissues such as lung, skeletal muscle and skin. Noggin has been shown to be a high-affinity BMP (bone morphogenetic protein) binding protein that antagonizes BMP bioactivities.

References
  1. Smith, W.C. and R.M. Harland (1992) Cell 70:829.
  2. Valenzuela, D.M. et al. (1995) J. Neurosci. 15:6077.
  3. Brunet, L.J. et al. (1998) Science 280:1455.
Entrez Gene IDs
9241 (Human); 18121 (Mouse)
Alternate Names
NOG; Noggin; SYM1; symphalangism 1 (proximal); synostoses (multiple) syndrome 1; SYNS1; SYNS1A

Citations for Recombinant Mouse Noggin Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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  1. iPS-cell-derived microglia promote brain organoid maturation via cholesterol transfer
    Authors: Park, DS;Kozaki, T;Tiwari, SK;Moreira, M;Khalilnezhad, A;Torta, F;Olivié, N;Thiam, CH;Liani, O;Silvin, A;Phoo, WW;Gao, L;Triebl, A;Tham, WK;Gonçalves, L;Kong, WT;Raman, S;Zhang, XM;Dunsmore, G;Dutertre, CA;Lee, S;Ong, JM;Balachander, A;Khalilnezhad, S;Lum, J;Duan, K;Lim, ZM;Tan, L;Low, I;Utami, KH;Yeo, XY;Di Tommaso, S;Dupuy, JW;Varga, B;Karadottir, RT;Madathummal, MC;Bonne, I;Malleret, B;Binte, ZY;Wei Da, N;Tan, Y;Wong, WJ;Zhang, J;Chen, J;Sobota, RM;Howland, SW;Ng, LG;Saltel, F;Castel, D;Grill, J;Minard, V;Albani, S;Chan, JKY;Thion, MS;Jung, SY;Wenk, MR;Pouladi, MA;Pasqualini, C;Angeli, V;Cexus, ONF;Ginhoux, F;
    Nature
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  2. ER stress induces upregulation of transcription factor Tbx20 and downstream Bmp2 signaling to promote cardiomyocyte survival
    Authors: S Das, A Mondal, C Dey, S Chakrabort, R Bhowmik, S Karmakar, A Sengupta
    The Journal of Biological Chemistry, 2023-02-16;0(0):103031.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Herpes Simplex Virus 1 and 2 Infections during Differentiation of Human Cortical Neurons
    Authors: P Bergström, E Trybala, CE Eriksson, M Johansson, TM Satir, S Widéhn, S Fruhwürth, W Michno, FH Nazir, J Hanrieder, SR Paludan, L Agholme, H Zetterberg, T Bergström
    Viruses, 2021-10-14;13(10):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Kinome-wide analysis of the effect of statins in colorectal cancer
    Authors: S Ouahoud, RJ Jacobs, MP Peppelenbo, GM Fühler, J Heijmans, S Diks, ME Wildenberg, LJAC Hawinkels, LL Kodach, PW Voorneveld, JCH Hardwick
    British Journal of Cancer, 2021-03-19;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Dual function of TGF? in lens epithelial cell fate: implications for secondary cataract
    Authors: BA Boswell, A Korol, JA West-Mays, LS Musil
    Mol. Biol. Cell, 2017-02-16;0(0):.
    Species: Chicken
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Amyloid precursor protein expression and processing are differentially regulated during cortical neuron differentiation
    Sci Rep, 2016-07-07;6(0):29200.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Abnormal Activation of BMP Signaling Causes Myopathy in Fbn2 Null Mice.
    Authors: Sengle G, Carlberg V, Tufa S, Charbonneau N, Smaldone S, Carlson E, Ramirez F, Keene D, Sakai L
    PLoS Genet, 2015-06-26;11(6):e1005340.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  8. A transient wave of BMP signaling in the retina is necessary for Muller glial differentiation.
    Authors: Ueki Y, Wilken M, Cox K, Chipman L, Bermingham-McDonogh O, Reh T
    Development, 2015-02-01;142(3):533-43.
    Species: Mouse
    Sample Types: In Vivo
  9. Wnt and BMP signaling cooperate with Hox in the control of Six2 expression in limb tendon precursor.
    Authors: Yamamoto-Shiraishi Y, Kuroiwa A
    2013-03-13;0(0):.
    Species: Mouse
    Sample Types: Embryo
    Applications: In Vivo
  10. Essential role of BMPs in FGF-induced secondary lens fiber differentiation.
    Authors: Boswell BA, Overbeek PA, Musil LS
    Dev. Biol., 2008-09-18;324(2):202-12.
    Species: Chicken
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Cross-talk between fibroblast growth factor and bone morphogenetic proteins regulates gap junction-mediated intercellular communication in lens cells.
    Authors: Boswell BA, Lein PJ, Musil LS
    Mol. Biol. Cell, 2008-04-09;19(6):2631-41.
    Species: Chicken
    Sample Types: Whole Tissue
    Applications: Bioassay
  12. Guided differentiation of embryonic stem cells into Pdx1-expressing regional-specific definitive endoderm.
    Authors: Shiraki N, Yoshida T, Araki K, Umezawa A, Higuchi Y, Goto H, Kume K, Kume S
    Stem Cells, 2008-01-31;26(4):874-85.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Activin-like kinase receptor 1 (ALK1) in atherosclerotic lesions and vascular mesenchymal cells.
    Authors: Yao Y, Zebboudj AF, Torres A, Shao E, Bostrom K
    Cardiovasc. Res., 2006-09-27;74(2):279-89.
    Species: Bovine, Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Canonical Wnt signaling is required for development of embryonic stem cell-derived mesoderm.
    Authors: Lindsley RC, Gill JG, Kyba M, Murphy TL, Murphy KM
    Development, 2006-08-30;133(19):3787-96.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Regulation of ADMP and BMP2/4/7 at opposite embryonic poles generates a self-regulating morphogenetic field.
    Authors: Reversade B, De Robertis EM
    Cell, 2005-12-16;123(6):1147-60.
    Species: Xenopus
    Sample Types: In Vivo
    Applications: In Vivo
  16. Requirements for endoderm and BMP signaling in sensory neurogenesis in zebrafish.
    Authors: Holzschuh J, Wada N, Wada C, Schaffer A, Javidan Y, Tallafuss A, Bally-Cuif L, Schilling TF
    Development, 2005-08-01;132(16):3731-42.
    Species: Xenopus
    Sample Types: In Vivo
    Applications: In Vivo
  17. Neural induction in Xenopus requires early FGF signalling in addition to BMP inhibition.
    Authors: Delaune E, Lemaire P, Kodjabachian L
    Development, 2004-12-08;132(2):299-310.
    Species: Xenopus
    Sample Types: In Vivo, Whole Tissue
    Applications: Bioassay, In Vivo
  18. The development of semicircular canals in the inner ear: role of FGFs in sensory cristae.
    Authors: Chang W, Brigande JV, Fekete DM, Wu DK
    Development, 2004-07-27;131(17):4201-11.
    Species: Chicken
    Sample Types: In Vivo
    Applications: In Vivo
  19. Timed interactions between the Hox expressing non-organiser mesoderm and the Spemann organiser generate positional information during vertebrate gastrulation.
    Authors: Wacker SA, Jansen HJ, McNulty CL, Houtzager E, Durston AJ
    Dev. Biol., 2004-04-01;268(1):207-19.
    Species: Xenopus
    Sample Types: In Vivo
    Applications: In Vivo
  20. Bone morphogenetic proteins are involved in fetal kidney tissue transplantation-induced neuroprotection in stroke rats.
    Authors: Chang CF, 107317, Morales M, Chou J, Chen HL, Hoffer B, Wang Y
    Alcohol and HIV-Derived Hepatocyte Apoptotic Bodies Induce Hepatic Stellate Cell Activation, 2002-09-01;43(3):418-26.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo

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