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Recombinant Mouse Nope/IGDCC4 Fc Chimera Protein, CF

Catalog #: 1394-NP
1 Citation Datasheet / COA / SDS
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1394-NP-050
R&D Systems Recombinant Proteins and Enzymes
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Nope/IGDCC4 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse (rm) Nope/IGDCC4 Fc Chimera at 10 µg/mL (100 µL/well) can bind rmNetrin-4 with a linear range of 8-250 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse Nope/IGDCC4 protein
Mouse Nope/IGDCC4
(Gly22-His 953)
Accession # Q9EQS9		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
Q9EQS9
N-terminal Sequence
Analysis
Gly22
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
128.2 kDa (monomer)
SDS-PAGE
160-170 kDa, reducing conditions

Product Datasheets

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1394-NP

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1394-NP

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Nope/IGDCC4

Mouse Nope (Neighbor Of Punc E11) was discovered as a gene proximal to the Punc gene on chromosome 9 (1). Punc and Nope are distant members of a subgroup of the immunoglobulin (Ig) superfamily, which include DCC (Deleted in Colorectal Cancer) (2), Caenorhabditis elegans UNC5 (UNC = behaviorally uncoordinated) and its mammalian homologues (rat UNC5H1 and H2, mouse UNC5H2 and H3, and human UNC5H3 and H4) (3), Drosophila Frazzled (4), vertebrate Neogenin (5), and mouse Nope and mouse Punc. Members of this subgroup of the Ig superfamily are type I transmembrane proteins with four Ig domains in their extracellular regions.

Mouse Nope consists of a 21 amino acid (aa) signal peptide, a 933 aa extracellular domain (including four Ig domains, five fibronectin-type III (FnIII) repeats), a 24 aa transmembrane segment, and a 274 aa cytoplasmic domain (1). The extracellular domain of mNope shares 45% aa sequence similarity with mouse Punc. However, the cytoplasmic domains of mNope and mouse Punc do not share aa sequence similarity. Compared to other members of the subgroup of the Ig superfamily, mouse Nope extracellular domain shares 25.8% and 25.2% aa similarity with mouse DCC and mouse Neogenin, respectively. Mouse and human Nope share 90.8% aa sequence similarity. Mouse Nope is expressed mostly in embryonic muscle tissues and in developing and adult nervous systems. The structural similarities between Nope and the guidance receptor of the DCC family suggest that Nope may have similar functions as the DCC family (6 - 8).

References
  1. Salbaum, J.M. and C. Kappen (2000) Genomics 64:15.
  2. Fearon et al. (1990) Science 247:49.
  3. Keino-Masu et al. (1996) Cell 87:175.
  4. Hong, K. et al. (1999) Cell 97:927.
  5. Leonardo, E.D. et al. (1997) Nature 386:833.
  6. Kolodziej et al. (1996) Cell 87:197.
  7. Meyerhardt (1997) Oncogene 14:1129.
  8. Vielmetter et al. (1997) Genomics 41:414.
Long Name
Neighbor of Punc E11/Immunoglobulin Superfamily, DCC Subclass, Member 4
Entrez Gene IDs
57722 (Human); 56741 (Mouse)
Alternate Names
DDM36; hDDM36; IGDCC4; immunoglobulin superfamily DCC subclass member 4; immunoglobulin superfamily, DCC subclass, member 4; KIAA1628; likely ortholog of mouse neighbor of Punc E11; LOC57722; Neighbor of punc e11; Nope; NOPEFLJ42051; Protein DDM36

Citation for Recombinant Mouse Nope/IGDCC4 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. WFIKKN2 is a bifunctional axon guidance cue that signals through divergent DCC family receptors
    Authors: Nickerson, KR;Tom, I;Cortés, E;Abolafia, JR;Özkan, E;Gonzalez, LC;Jaworski, A;
    bioRxiv : the preprint server for biology
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: Bioassay

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