Recombinant Mouse NrCAM Protein, CF Summary
Product Specifications
Leu30-Pro630, with a C-terminal 6-His tag
Analysis
Customers also Viewed
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
8425-NR
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 500 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: NrCAM
NrCAM, also known as Bravo, belongs to the L1 family of cell adhesion molecules which also includes L1CAM, Neurofascin, and CHL-1/L1CAM-2 (1). These molecules are type I transmembrane proteins that have 6 Ig-like domains and 4-5 fibronectin type III-like domains in their extracellular domain. L1 family cell adhesion molecules are expressed primarily in the nervous system where they share overlapping functions in controlling axonal growth and guidance (2). Mature mouse NrCAM is an approximately 200 kDa molecule that consists of a 1090 amino acid (aa) extracellular domain (ECD) with 6 Ig-like domains followed by 4 fibronectin type III domains; a 23 aa transmembrane segment, and a 114 aa cytoplasmic domain. Within the region of Ig-like domains, mouse NrCAM shares 91% and 96% aa sequence identity with human and rat NrCAM, respectively. Alternative splicing generates an additional isoform with short deletions in the sixth Ig-like domain and in the juxtamembrane region of the ECD. A 140 kDa soluble fragment of the ECD can be released by proteolytic cleavage (3, 4). NrCAM is expressed on cerebellar granule neurons, retinal ganglion cells (RGC), star pyramidal cells in visual cortex, thalamocortical axons, and glial cells (3, 5-10). It is found on both axons and dendritic spines (6, 8). NrCAM mediates homophilic adhesion as well as heterophilic adhesion with Contactin, Contactin-2/TAG1, Neurofascin, PTP beta zeta, and Integrin alpha 4 beta 1 (4, 11-14) and also interacts with Neuropilin-2, Plexin A3, and EphB2 (7-9). Depending on its interacting partners, NrCAM can promote or inhibit axon and neurite extension (5, 6, 10, 14) and mediate Semaphorin 3F induced neuronal growth cone collapse (8, 9). NrCAM plays an important role in the development of normal vision by regulating RGC axon pathfinding and mapping to the visual cortex (6, 7, 9). It is upregulated in papillary thyroid carcinomas and the shed form can promote tumorigenesis (4, 15).
- Sakurai, T. (2012) Mol. Cell. Neurosci. 49:351.
- Stoeckli, E.T. and L.T. Landmesser (1995) Neruon 14:1165.
- Sakurai, T. et al. (2001) J. Cell Bio. 154:1259.
- Conacci-Sorrell, M. et al. (2005) Cancer Res. 65:11605.
- Faivre-Sarrailh, C. et al. (1999) J. Cell Sci. 112:3015.
- Zelina, P. et al. (2005) Development 132:3609.
- Dai, J. et al. (2013) PLoS One 8:e73000.
- Demyanenko, G.P. et al. (2014) J. Neurosci. 34:11274.
- Demyanenko, G.P. et al. (2011) J. Neurosci. 31:1545.
- Feinberg, K. et al. (2010) Neuron 65:490.
- Mauro, V.P. et al. (1992) J. Cell Biol. 119:191.
- Sakurai, T. et al. (1997) J. Cell Biol. 136:907.
- Lustig, M. et al. (1999) Dev. Biol. 209:340.
- Volkmer, H. et al. (1996) J. Cell Biol. 135:1059.
- Gorka, B. et al. (2007) Br. J. Cancer 97:531.
Citation for Recombinant Mouse NrCAM Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
-
Secretomics reveals gelatinase substrates at the blood-brain barrier that are implicated in astroglial barrier function
Authors: Burmeister, M;Fraunenstein, A;Kahms, M;Arends, L;Gerwien, H;Deshpande, T;Kuhlmann, T;Gross, CC;Naik, VN;Wiendl, H;Klingauf, J;Meissner, F;Sorokin, L;
Science advances
Species: Mouse
Sample Types: Recombinant Protein
Applications: Bioassay
FAQs
No product specific FAQs exist for this product, however you may
View all Proteins and Enzyme FAQsReviews for Recombinant Mouse NrCAM Protein, CF
There are currently no reviews for this product. Be the first to review Recombinant Mouse NrCAM Protein, CF and earn rewards!
Have you used Recombinant Mouse NrCAM Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
