Recombinant Mouse OSCAR Fc Chimera Protein, CF

Catalog #: 1633-OS Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
1633-OS-050

Recombinant Mouse OSCAR Fc Chimera Protein Binding Activity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse OSCAR Fc Chimera Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Bovine Collagen I is coated at 10 µg/mL, Recombinant Mouse OSCAR Fc Chimera binds with an ED₅₀ of 80-640 ng/mL.

Source

Mouse myeloma cell line, NS0-derived mouse OSCAR protein

Mouse OSCAR (Asp19-Asn228) Accession # Q8VBT3	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

Q8VBT3

N-terminal Sequence
Analysis

Asp19

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

49 kDa

SDS-PAGE

56-66 kDa, reducing conditions

Product Datasheets

1633-OS

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1633-OS

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	12 months from date of receipt, ≤ -20 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Bovine College I is coated at 10 µg/mL, Recombinant Mouse OSCAR Fc Chimera binds with an ED₅₀ of 80-640 ng/mL.

Reconstitution Calculator

Background: OSCAR

OSCAR (Osteoclast-associated immunoglobulin-like receptor), is a type I transmembrane protein of the leukocyte receptor complex (LRC) family (1). The mature mouse OSCAR includes a 213 amino acid (aa) extracellular domain (ECD) with two Ig domains homology to PIR family proteins, a 17 aa transmembrane region, and a 17 aa cytoplasmic domain. The ECD of mouse OSCAR shares 77% and 84% aa sequence identity with the ECD of human and rat OSCAR, respectively (2). OSCAR is specifically expressed by pre-osteoclasts and signals via FcR gamma (3). OSCAR co-stimulates one of the major FcR gamma‑associated pathways required for osteoclastogenesis in vivo (3). Collagen type I and collagen type II serve as ligands for OSCAR (4). OSCAR binds to specific motifs within fibrillary collagens in the extracellular matrix (ECM) (3). OSCAR promoted osteoclastogenesis in vivo, and its binding to the collagen motif led to signaling that increased numbers of osteoclasts in culture (3). OSCAR contains two Ig domains, D1 and D2. Direct binding assays on OSCAR molecules showed that D2, but not D1, is critical for collagen binding (5). OSCAR may contribute to the pathogenesis and severity of a number of diseases including osteoporosis, atherosclerosis, chronic obstructive pulmonary disease and rheumatoid arthritis (5).

References

Kim, N. et al. (2002) J. Exp. Med. 195:201.
Haywood, J. et al. (2016) Proc. Natl. Acad. Sci. USA 113:1038.
Barrow, A.D. et al. (2011) J. Clin. Invest. 121:3505.
Shultz, H.S. et al. (2016) Eur. J. Immunol. 46:952.
Zhou, L. et al. (2016) Blood 127:529.

Long Name

Osteoclast-associated Receptor

Entrez Gene IDs

126014 (Human); 232790 (Mouse)

Alternate Names

hOSCAR; MGC33613; OSCAR; osteoclast associated receptor OSCAR-S1; osteoclast associated receptor OSCAR-S2; osteoclast associated, immunoglobulin-like receptor; osteoclast-associated immunoglobulin-like receptor; Osteoclast-associated receptor; PIGR3; PIgR-3; Poly-Ig receptor 3; Polymeric immunoglobulin receptor 3