Recombinant Mouse PD-1 His-tag Protein, CF

Catalog #: 9047-PD
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9047-PD-100

Surface plasmon resonance sensorgram of mouse PD-L1 protein binding to mouse PD-1

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse PD-1 His-tag Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse PD-1 is immobilized at 2 µg/mL (100 µL/well), the concentration of Recombinant Mouse B7‑H1/PD-L1 Fc Chimera (Catalog # 1019-B7) that produces 50% of the optimal binding response is approximately 0.75-3.75 μg/mL.

Source

Mouse myeloma cell line, NS0-derived mouse PD-1 protein
Leu25 - Gln167, with a C-terminal 6-His tag

Accession #

Q02242

N-terminal Sequence
Analysis

Leu25

Predicted Molecular Mass

17 kDa

SDS-PAGE

36 - 46 kDa, reducing conditions

Product Datasheets

9047-PD

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9047-PD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Surface Plasmon Resonance (SPR)

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Recombinant Mouse PD-L1/B7-H1 Fc protein (1019-B7) was immobilized on a Biacore Sensor Chip CM5, and binding to Recombinant Mouse PD-1 His protein (Catalog # 9047-PD) was measured at a concentration range between 1.53 nM and 1.56 uM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=0.539 uM.

Reconstitution Calculator

Background: PD-1

Programmed Death-1 receptor (PD-1), also known as CD279, is type I transmembrane protein belonging to the CD28 family of immune regulatory receptors (1). Other members of this family include CD28, CTLA-4, ICOS, and BTLA (2-5). Mature mouse PD-1 consists of a 149 amino acid (aa) extracellular region (ECD) with one immunoglobulin-like V-type domain, a 21 aa transmembrane domain, and a 98 aa cytoplasmic region. The mouse PD-1 ECD shares 65% aa sequence identity with the human PD-1 ECD. The cytoplasmic tail contains two tyrosine residues that form the immunoreceptor tyrosine-based inhibitory motif (ITIM) and immunoreceptor tyrosine-based switch motif (ITSM) that are important for mediating PD-1 signaling. PD-1 acts as a monomeric receptor and interacts in a 1:1 stoichiometric ratio with its ligands PD-L1 (B7-H1) and PD-L2 (B7-Dc) (6, 7). PD‑1 is expressed on activated T cells, B cells, monocytes, and dendritic cells while PD-L1 expression is constitutive on the same cells and also on nonhematopoietic cells such as lung endothelial cells and hepatocytes (8, 9). Ligation of PD-L1 with PD-1 induces co-inhibitory signals on T cells promoting their apoptosis, anergy, and functional exhaustion (10). Thus, the PD-1:PD-L1 interaction is a key regulator of the threshold of immune response and peripheral immune tolerance (11). Finally, blockade of the PD-1: PD-L1 interaction by either antibodies or genetic manipulation accelerates tumor eradication and shows potential for improving cancer immunotherapy (12-14).

References

Ishida, Y. et al. (1992) EMBO J. 11:3887.
Sharpe, A.H. and G. J. Freeman (2002) Nat. Rev. Immunol. 2:116.
Coyle, A. and J. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
Nishimura, H. and T. Honjo (2001) Trends Immunol. 22:265.
Watanabe, N et al. (2003) Nat. Immunol. 4:670.
Zhang, X. et al. (2004) Immunity 20:337.
Lázár-Molnár, E. et al. (2008) Proc. Natl. Acad. Sci. USA 105:10483.
Nishimura, H et al. (1996) Int. Immunol. 8:773.
Keir, M.E. et al. (2008) Annu. Rev. Immunol. 26:677.
Butte, M.J. et al. (2007) Immunity 27:111.
Okazaki, T. et al. (2013) Nat. Immunol. 14:1212.
Iwai, Y. et al. (2002) Proc. Natl. Acad. Sci. USA 99: 12293.
Nogrady, B. (2014) Nature 513:S10.
Chen, L. and X. Han (2015) J. Clin. Invest. 125:3384.

Long Name

Programmed Death-1

Entrez Gene IDs

5133 (Human); 18566 (Mouse); 301626 (Rat); 100533201 (Porcine); 486213 (Canine); 102123659 (Cynomolgus Monkey)

Alternate Names

CD279 antigen; CD279; hPD-1; PD1; PD-1; PD1hPD-l; PDCD1; programmed cell death 1; programmed cell death protein 1; Protein PD-1; SLEB2

Related Research Areas

Citation for Recombinant Mouse PD-1 His-tag Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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