Recombinant Mouse PD-L1/B7-H1 His-tag Avi-tag Protein, CF

Catalog #: AVI9048 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AVI9048-050

Biotinylated Recombinant Mouse PD-L1/B7-H1 His-tag Avi-tag Protein Binding Activity.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse PD-L1/B7-H1 His-tag Avi-tag Protein, CF Summary

Learn more about Avi-tag Biotinylated Proteins

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. Biotinylated Recombinant Mouse PD-L1/B7-H1 His-tag Avi-tag (Catalog # AVI9048) binds to Recombinant Mouse PD-1 Fc Chimera Protein (Catalog # 1021-PD) with an ED₅₀ of 15.0-150 ng/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived mouse PD-L1/B7-H1 protein

Mouse B7-H1 (Phe19-His239) Accession # Q9EP73.1	6-His tag	Avi-tag
N-terminus		C-terminus

Accession #

Q9EP73.1

N-terminal Sequence
Analysis

Phe19

Structure / Form

Biotinylated via Avi-tag

Predicted Molecular Mass

28 kDa

SDS-PAGE

40-58 kDa, under reducing conditions.

Product Datasheets

AVI9048

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI9048

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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Measured by its binding ability in a functional ELISA. Biotinylated Recombinant Mouse PD-L1/B7-H1 His-tag Avi-tag Protein (Catalog # AVI9048) binds to Recombinant Mouse PD-1 Fc Chimera Protein (1021-PD) with an ED₅₀ of 15.0‑150 ng/mL.

SDS-PAGE

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2 μg/lane of Biotinylated Recombinant Mouse PD-L1/B7-H1 His-tag Avi-tag Protein (Catalog # AVI9048) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 40-58 kDa.

Reconstitution Calculator

Background: PD-L1/B7-H1

B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules (1). Mature mouse B7-H1 consists of a 221 amino acid (aa) extracellular domain (ECD) with two immunoglobulin-like domains, a 21 aa transmembrane segment, and a 30 aa cytoplasmic domain (2). Within the ECD, mouse B7-H1 shares 73% and 86% aa sequence identity with human and rat B7-H1, respectively. B7-H1 is expressed on inflammatory-activated immune cells including macrophages, T cells, and B cells (2-5), keratinocytes (6, 7), enothelial and intestinal epithelial cells (6, 8), as well as a variety of carcinomas and melanoma (9, 10). B7-H1 binds to T cell B7-1/CD80 and PD-1 (5, 6, 10-13). It suppresses T cell activation and proliferation (3, 6, 12, 14) and induces the apoptosis of activated T cells (9). It plays a role in the development of immune tolerance by promoting T cell anergy (5, 12) and enhancing regulatory T cell development (14). B7-H1 favors the development of anti-inflammatory IL-10 and IL-22 producing dendritic cells (3, 8) and inhibits the development of Th17 cells (14). In cancer, B7-H1 provides resistance to T cell mediated lysis, enhances EMT, and enhances the tumorigenic function of Th22 cells (4, 7, 10, 13). Our Avi-tag Biotinylated mouse B7-H1 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

Ceeraz, S. et al. (2013) Trends Immunol. 34:556.
Tamura, H. et al. (2001) Blood 97:1809.
Chen, L. et al. (2007) J. Immunol. 178:6634.
Kuang, D.-M. et al. (2014) J. Clin. Invest. 124:4657.
Tsushima, F. et al. (2007) Blood 110:180.
Mazanet, M.M. and C.C.W. Hughes (2002) J. Immunol. 169:3581.
Cao, Y. et al. (2010) Cancer Res. 71:1235.
Scandiuzzi, L. et al. (2014) Cell Rep. 6:625.
Dong, H. et al. (2002) Nat. Med. 8:793.
Azuma, T. et al. (2008) Blood 111:3635.
Butte, M.J. et al. (2008) Mol. Immunol. 45:3567.
Park, J.-J. et al. (2010) Blood 116:1291.
Ritprajak, P. et al. (2010) J. Immunol. 184:4918.
Herold, M. et al. (2015) J. Immunol. 195:3584.

Long Name

Programmed Death Ligand 1

Entrez Gene IDs

29126 (Human); 60533 (Mouse); 499342 (Rat); 574058 (Porcine); 484186 (Canine); 102145573 (Cynomolgus Monkey)

Alternate Names

Avelumab; B7-H; B7H1; B7-H1; B7H1PDCD1L1; CD274 antigenMGC142294; CD274 molecule; CD274; PDCD1L1; PDCD1LG1; PDCD1LG1MGC142296; PDL1; PD-L1; PD-L1B7 homolog 1; PDL1PDCD1 ligand 1; programmed cell death 1 ligand 1; Programmed death ligand 1