Recombinant Mouse PILR-beta Protein, CF

Catalog #: 3525-PR Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
3525-PR-050

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Recombinant Mouse PILR-beta Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to bind Recombinant Mouse CD99 Fc Chimera (Catalog # 3905-CD) in a functional ELISA. Shiratori, I. et al. (2004) J Exp. Med. 199:525.

Source

Mouse myeloma cell line, NS0-derived mouse PILR-beta protein
Ala28-Gly193, with a C-terminal 6-His tag

Accession #

NP_573472

N-terminal Sequence
Analysis

Ala28

Predicted Molecular Mass

19.6 kDa

SDS-PAGE

39-44 kDa, reducing conditions

Product Datasheets

3525-PR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3525-PR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: PILR-beta

PILR-beta (paired immunoglobulin-like type 2 receptor-beta) is one of two members of a small family of immunoregulatory Ig-superfamily receptors (1, 2). It is a counterpart to PILR-alpha and it likely arose through PILR-alpha gene duplication and rearrangement (1). The PILRs represent one of many pairs of Ig-like domain-containing receptors that participate in immune regulation. PILR-beta and -alpha should not be confused with the similarly named PIRs (also paired immunoglobulin-like receptors), or the functionally-related SIRP and ILT/LILR/CD85/LIR family of receptors (2). While PIRs, ILTs and SIRPs contain three to six Ig-like domains in their extracellular region, PILR-beta and -alpha show only one Ig-like region in their extracellular domain (ECD) (1, 2). Mouse PILR-beta is a 196 amino acid (aa) type I transmembrane (TM) protein (3). It contains a 167 aa ECD, a 21 aa TM segment, and a short 8 aa cytoplasmic region. The ECD shows a V-type Ig-like domain (aa 39 - 135), while the TM segment contains a positively-charged Lys at position # 202. This Lys is known to interact with the transmembrane signaling adaptor protein DAP12, making PILR-beta an activating receptor. Activation of PILR-beta through CD99 ligation induces NK cell cytotoxicity and dendritic cell secretion of NO and TNF-alpha (3). Mouse PILR-beta is found on NK cells, neutrophils, macrophages, and monocyte-derived dendritic cells (3). Mouse PILR-beta ECD is 44% and 75% aa identical to human and rat PILR-beta ECD, respectively; it is 75% aa identical to the ECD of mouse PILR-alpha (3). Evidence suggests that mouse PILR-beta will not be active in a human system (3). Potential isoforms of PILR-beta have been reported. One shows an alternate start site at Met14, a second shows a 21 aa substitution for the C-terminal 67 aa, and a third exhibits multiple polymorphisms for an overall aa identity of 86% (4, 5, 6).

References

Wilson, M.D. et al. (2006) Physiol. Genomics 27:201.
Lanier, L.L. (2001) Curr. Opin. Immunol. 13:326.
Shiratori, I. et al. (2004) J. Exp. Med. 199:525.
Genbank Accession # NP_573472.
Genbank Accession # BAC29442.
Genbank Accession # XP_001480583.

Long Name

Paired-Ig-likeType 2 Receptor beta

Entrez Gene IDs

29990 (Human); 170741 (Mouse)

Alternate Names

activating receptor PILRbeta; Activating receptor PILR-beta; Cell surface receptor FDFACT; cell surface receptor FDFACT1; cell surface receptor FDFACT2; FDFACT; FDFACT1; FDFACT2; paired immunoglobin-like receptor beta; paired immunoglobin-like type 2 receptor beta; paired immunoglobulin-like receptor beta; paired immunoglobulin-like type 2 receptor beta; PILRB; PILRbeta; PILR-beta