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Recombinant Mouse Podoplanin Fc Chimera Protein, CF

Catalog #: 3244-PL
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
3244-PL-050
R&D Systems Recombinant Proteins and Enzymes
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Citations (3)
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Recombinant Mouse Podoplanin Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When 0.5 μg/mL of rmPodoplanin/Fc is immobilized onto a Goat Anti-Mouse IgG Fc chimera (R&D Systems, Catalog # G-202-C) coated plate, the concentration of rhCLEC-2 that produces 50% of the optimal binding response is found to be approximately 0.3-1.8 nM.
Source
Mouse myeloma cell line, NS0-derived mouse Podoplanin protein
Mouse Podoplanin
(Gln21-Lys133)
Accession # Q62011		 IEGRMDP Mouse IgG2A
(Glu98-Lys330)
N-terminus C-terminus
Accession #
Q62011
N-terminal Sequence
Analysis
Results not obtained: Gln21 predicted
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
19.0 kDa (monomer)
SDS-PAGE
45-70 kDa, reducing conditions

Product Datasheets

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3244-PL

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3244-PL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Podoplanin

Podoplanin, also known as glycoprotein 38 (gp38), PA2.26 antigen, T1-alpha (T1A), and aggrus, is a 38 kDa type I transmembrane sialoglycoprotein and member of the podoplanin family (1, 2). Podoplanin is synthesized as a 172 amino acid (aa) precursor with a 22 aa signal sequence, a 119 aa extracellular domain (ECD), a 21 aa transmembrane region, and a short, 10 aa cytoplasmic tail (SwissProt #: Q62011). The ECD contains abundant Ser/Thr residues as potential sites for O-glycosylation, and the cytoplasmic region contains putative sites for kinase C and cAMP phosphorylation (2, 3). Mouse Podoplanin shares 77% and 46% aa sequence identity with rat and human Podoplanin, respectively. Podoplanin is expressed on glomerular epithelial cells (podocytes), type I lung alveolar cells, lymphatic endothelial cells (4, 5), and on numerous tumors including colorectal tumors (3), squamous cell carcinomas (4, 6), testicular seminoma (7), and brain tumors (8-10). One study shows high expression of Podoplanin mRNA in placenta, lung, skeletal muscle, and heart, and weaker levels in brain, kidney, and liver (1). Podoplanin is the ligand for C-type lectin-like receptor 2 (CLEC-2) (2). Their association is dependent on sialic acid on O-glycans of Podoplanin (2). Through its association with CLEC-2, Podoplanin induces platelet aggregation and tumor metastasis (2). Podoplanin is also necessary for lymphatic vessel formation, normal lung cell proliferation and alveolus formation at birth (2).

References
  1. Zimmer, G. et al. (1999) Biochem. J. 341:277.
  2. Katsue-Inoue, K. et al. (2007) J. Biol. Chem. 282:25993.
  3. Kato, Y. et al. (2003) J. Biol. Chem. 278:51599.
  4. Schacht, V. et al. (2005) Am. J. Pathol. 166:913.
  5. Breiteneder-Geleff, S. et al. (1997) Am. J. Pathol. 151:1141.
  6. Kato, Y. et al. (2005) Tumour Biol. 26:195.
  7. Kato, Y. et al. (2004) Oncogene 23:8552.
  8. Mishima, K. et al. (2006) Acta Neuropathol. 111:563.
  9. Mishima, K. et al. (2006) Acta Neuropathol. 111:483.
  10. Kato, Y. et al. (2006) Biochem. Biophys. Res. Commun. 349:1301.
Long Name
Lung Type-I Cell Membrane-associated Glycoprotein, Isoform A
Entrez Gene IDs
10630 (Human); 14726 (Mouse); 54320 (Rat)
Alternate Names
36-KD; Aggrus; Gp36; Gp38; GP40; HT1A-1; hT1alpha-1; hT1alpha-2; lung type I cell membrane associated glycoprotein; lung type-I cell membrane-associated glycoprotein (T1A-2); OTS 8; OTS8; PA2.26 antigen; PDPN; Podoplanin; RANDAM-2; T1A; T1A-2; T1-alpha

Citations for Recombinant Mouse Podoplanin Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Monocyte upregulation of podoplanin during early sepsis induces complement inhibitor release to protect liver function
    Authors: Z Xie, B Shao, C Hoover, M McDaniel, J Song, M Jiang, Z Ma, F Yang, J Han, X Bai, C Ruan, L Xia
    JCI Insight, 2020-07-09;5(13):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Structural and functional features of central nervous system lymphatic vessels.
    Authors: Louveau A, Smirnov I, Keyes T, Eccles J, Rouhani S, Peske J, Derecki N, Castle D, Mandell J, Lee K, Harris T, Kipnis J
    Nature, 2015-06-01;523(7560):337-41.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Antibody Absorption
  3. Platelet ITAM signaling is critical for vascular integrity in inflammation.
    Authors: Boulaftali, Yacine, Hess, Paul R, Getz, Todd M, Cholka, Agnieszk, Stolla, Moritz, Mackman, Nigel, Owens, A Philli, Ware, Jerry, Kahn, Mark L, Bergmeier, Wolfgang
    J Clin Invest, 2013-01-25;123(2):908-16.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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