Recombinant Mouse Proprotein Convertase 9/PCSK9 Protein, CF
Recombinant Mouse Proprotein Convertase 9/PCSK9 Protein, CF Summary
Product Specifications
9258-SE) binds with an ED50 = 25-150 ng/mL.
Ser156-Gln694 with a C-terminal 10-His tag; Gln35-Gln155
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9258-SE
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: |
Scientific Data
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Recombinant Mouse Proprotein Convertase 9/ PCSK9 Binds to Recombinant Mouse LDL R in an ELISA Binding Assay. When Recombinant Mouse LDL R Recombinant Mouse LDL R (Catalog # 2255-LD) is coated at 2 µg/mL, Recombinant Mouse Proprotein Convertase 9/PCSK9 (Catalog # 9258-SE) binds with an ED50 = 25-150 ng/mL.
Reconstitution Calculator
Background: Proprotein Convertase 9/PCSK9
PCSK9 (proprotein convertase subtilisin kexin 9), also known as NARC-1, is a member of the proteinase K subfamily of subtilisin-related serine endoproteases. It is highly expressed in the liver, intestine, and kidney and plays an important role in regulating LDL R expression and circulating cholesterol levels (1). PCSK9 is synthesized as precursor protein that is autocatalytically cleaved in the endoplasmic reticulum to generate a 14 kDa prodomain and a 60 kDa catalytic domain (2). Within the secretion pathway, the prodomain remains associated with and functions as a chaperone for the catalytic domain. Mouse PCSK9 shares 78% and 93% amino acid identity with human and rat PCSK9, respectively. PCSK9 plays a key role in the regulation of cholesterol metabolism by binding to hepatic LDL R, LRP-1, VLDL R, and Apolipoprotein E R2 and promoting their lysosomal degradation instead of recycling to the plasma membrane (3-8). It can also regulate cholesterol and triglyceride handling in the intestine and adipose tissue (9-11). The ability of PCSK9 to regulate LDL R expression is inhibited by its binding to LDL particles or Annexin A2 or by additional proteolytic cleavage (12-17).
- Schulz, R. et al. (2015) Basic Res. Cardiol. 110:4.
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- Lagace, T.A. et al. (2006) J. Clin. Invest. 116:2995.
- Grefhorst, A. et al. (2008) J. Lipid Res. 49:1303.
- Zhang, D.W. (2007) J. Biol. Chem. 282:18602.
- DeVay, R.M. et al. (2013) J. Biol. Chem. 288:10805.
- Canuel, M. et al. (2013) PLoS One 8:e64145.
- Poirier, S. et al. (2008) J. Biol. Chem. 283:2363.
- Levy, E. et al. (2013) Atherosclerosis 227:297.
- Le May, C. et al. (2013) Arterioscler. Thromb. Vasc. Biol. 33:1484.
- Roubtsova, A. et al. (2011) Arterioscler. Thromb. Vasc. Biol. 31:785.
- Kosenko, T. et al. (2013) J. Biol. Chem. 288:8279.
- Mayer, G. et al. (2008) J. Biol. Chem. 283:31791.
- Benjannet, S. et al. (2006) J. Biol. Chem. 281:30561.
- Benjannet, S. et al. (2010) J. Biol. Chem. 285:40965.
- Essalmani, R. et al. (2011) J. Biol. Chem. 286:4257.
- Han, B. et al. (2014) J. Lipid Res. 55:1505.
Citations for Recombinant Mouse Proprotein Convertase 9/PCSK9 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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A Virus-like particle-based bivalent PCSK9 vaccine lowers LDL-cholesterol levels in Non-Human Primates
Authors: Fowler, A;Van Rompay, KKA;Sampson, M;Leo, J;Watanabe, JK;Usachenko, JL;Immareddy, R;Lovato, DM;Schiller, JT;Remaley, AT;Chackerian, B;
bioRxiv : the preprint server for biology
Species: Primate - Macaca fascicularis (Cynomolgus Macaque)
Sample Types: Serum
Applications: ELISA Capture -
Rosa26-LSL-dCas9-VPR: a versatile mouse model for tissue specific and simultaneous activation of multiple genes for drug discovery
Authors: D Pakalnišky, T Schönberge, B Strobel, B Stierstorf, T Lamla, M Schuler, M Lenter
Scientific Reports, 2022-11-10;12(1):19268.
Species: N/A
Sample Types: Buffer
Applications: Western Blot -
All-in-one adeno-associated virus delivery and genome editing by Neisseria meningitidis Cas9 in vivo
Authors: R Ibraheim, CQ Song, A Mir, N Amrani, W Xue, EJ Sontheimer
Genome Biol., 2018-09-19;19(1):137.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
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