Home / R-Spondin 1 / Recombinant Mouse R-Spondin 1 Protein, CF

Recombinant Mouse R-Spondin 1 Protein, CF

Catalog #: 3474-RS
69 Citations 5 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
3474-RS-050
3474-RS-01M
3474-RS-250
Recombinant Mouse R-Spondin 1 Protein Bioactivity
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Product Details
Citations (69)
FAQs
Supplemental Products
Reviews (5)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse R-Spondin 1 Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The typical ED50 is 50-200 ng/mL in the presence of 5 ng/mL recombinant mouse Wnt-3a.
Source
E. coli-derived mouse R-Spondin 1 protein
Ser21-Gly209, with an N-terminal Met
Accession #
Q9Z132
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
21 kDa

Product Datasheets

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3474-RS

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3474-RS

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Mouse R-Spondin 1 Protein Bioactivity View Larger

Recombinant Mouse R-Spondin 1 (Catalog # 3474-RS) induces activation of beta -catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is 50-200 ng/mL in the presence of 5 ng/mL of Recombinant Mouse Wnt-3a (Catalog # 1324-WN).

SDS-PAGE Recombinant Mouse R-Spondin 1 Protein SDS-PAGE View Larger

1 μg/lane of Recombinant Mouse R-Spondin-1 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 24 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: R-Spondin 1

R-Spondin 1 (RSPO1, Roof plate-specific Spondin 1), also known as cysteine-rich and single thrombospondin domain containing protein 3 (Cristin 3), is a 27 kDa secreted protein that shares ~40% amino acid (aa) identity with three other R-Spondin family members (1, 2). All R-Spondins regulate Wnt/ beta-Catenin signaling but have distinct expression patterns (1-3). R-Spondin 1 competes with the Wnt antagonist DKK-1 for binding to the Wnt co-receptors, Kremen and LRP-6, reducing their DKK-1-mediated internalization (4). However, reports are mixed on whether R-Spondin 1 binds LRP-6 directly (4-6). R-Spondin 1 is expressed in early development at the roof plate boundary and is thought to contribute to dorsal neural tube development (3, 7). In humans, rare disruptions of the R-Spondin 1 gene are associated with tendencies for XX sex reversal (phenotypic male) or hermaphroditism, indicating a role for R-Spondin 1 in gender-specific differentiation (7, 8). Mutations in R-Spondin 1 are also linked with palmoplantar keratoderma, abnormal thickening of the skin on the palms of the hands and soles of the feet (7, 8). Postnatally, R-Spondin 1 is expressed by neuroendocrine cells in the intestine, adrenal gland and pancreas, and by epithelia in kidney and prostate (9). Injection of recombinant R-Spondin 1 in mice causes activation of beta-catenin and proliferation of intestinal crypt epithelial cells, and ameliorates experimental colitis (9, 10). Interest in R-Spondin 1 as a cell culture supplement has grown with the expansion of the organoid field. R-Spondin 1 is widely used in organoid cell culture workflows as a vital component that promotes both growth and survival of 3D organoids (11).

Structurally similar to other R-Spondins, R-Spondin 1 contains two adjacent cysteine-rich furin-like domains (aa 34-135) with one potential N-glycosylation site, followed by a thrombospondin (TSP-1) motif (aa 147-207) and a region rich in basic residues (aa 211-263). Only the furin-like domains are needed for beta-catenin stabilization (2, 12). A putative nuclear localization signal at the C-terminus may allow some expression in the nucleus (13). Mouse R‑Spondin 1 shares 98%, 94%, 94%, 93%, 92% and 88% aa identity with rat, human, horse, cow, goat and dog RSPO-1, respectively, within aa 21‑209.

References
  1. Chen, J-Z. et al. (2002) Mol. Biol. Rep. 29:287.
  2. Kim, K.-A. et al. (2006) Cell Cycle 5:23.
  3. Nam, J.-S. et al. (2007) Gene Expr. Patterns 7:306.
  4. Binnerts, M.E. et al. (2007) Proc. Natl. Acad. Sci. USA 104:14700.
  5. Nam, J.-S. et al. (2006) J. Biol. Chem. 281:13247.
  6. Wei, Q. et al. (2007) J. Biol. Chem. 282:15903.
  7. Kamata, T. et al. (2004) Biochim. Biophys. Acta 1676:51.
  8. Parma, P. et al. (2006) Nat. Genet. 38:1304.
  9. Kim, K.-A. et al. (2005) Science 309:1256.
  10. Zhao, J. et al. (2007) Gastroenterology 132:1331.
  11. Drost and Clevers. (2018) Nature Reviews Cancer 18:407.
  12. Kazanskaya, O. et al. (2004) Dev. Cell 7:525.
  13. Tomaselli, S. et al. (2008) Hum. Mutat. 29:220.
Long Name
Roof Plate-specific Spondin 1
Entrez Gene IDs
284654 (Human); 192199 (Mouse); 102122369 (Cynomolgus Monkey)
Alternate Names
Cristin 3; CRISTIN3; FLJ40906Roof plate-specific spondin-1; HRspo1; roof plate-specific spondin; RSPO; RSPO1; RSpondin 1; R-Spondin 1; R-spondin homolog (Xenopus laevis); RSPONDIN; R-spondin1; R-spondin-1

Citations for Recombinant Mouse R-Spondin 1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

69 Citations: Showing 1 - 10
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  1. An Adaptable Protocol to Generate a Murine Enteroid-Macrophage Co-Culture System
    Authors: Hentschel, V;Govindarajan, D;Seufferlein, T;Armacki, M;
    International journal of molecular sciences
    Species: Mouse
    Sample Types: Enteroid
    Applications: Bioassay
  2. LGR signaling mediates muscle-adipose tissue crosstalk and protects against diet-induced insulin resistance
    Authors: Kubrak, O;Jørgensen, AF;Koyama, T;Lassen, M;Nagy, S;Hald, J;Mazzoni, G;Madsen, D;Hansen, JB;Larsen, MR;Texada, MJ;Hansen, JL;Halberg, KV;Rewitz, K;
    Nature communications
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. The porcine islet-derived organoid showed the characteristics as pancreatic duct
    Authors: Sakata, N;Yoshimatsu, G;Kawakami, R;Nakano, K;Yamada, T;Yamamura, A;Nagashima, H;Kodama, S;
    Scientific reports
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Pathway level subtyping identifies a slow-cycling biological phenotype associated with poor clinical outcomes in colorectal cancer
    Authors: Malla, SB;Byrne, RM;Lafarge, MW;Corry, SM;Fisher, NC;Tsantoulis, PK;Mills, ML;Ridgway, RA;Lannagan, TRM;Najumudeen, AK;Gilroy, KL;Amirkhah, R;Maguire, SL;Mulholland, EJ;Belnoue-Davis, HL;Grassi, E;Viviani, M;Rogan, E;Redmond, KL;Sakhnevych, S;McCooey, AJ;Bull, C;Hoey, E;Sinevici, N;Hall, H;Ahmaderaghi, B;Domingo, E;Blake, A;Richman, SD;Isella, C;Miller, C;Bertotti, A;Trusolino, L;Loughrey, MB;Kerr, EM;Tejpar, S;Maughan, TS;Lawler, M;Campbell, AD;Leedham, SJ;Koelzer, VH;Sansom, OJ;Dunne, PD;
    Nature genetics
    Species: Mouse
    Sample Types: Organoid
    Applications: Bioassay
  5. Microbial-dependent recruitment of immature myeloid cells promotes intestinal regeneration
    Authors: Jiang, Z;Waterbury, QT;Malagola, E;Fu, N;Kim, W;Ochiai, Y;Wu, F;Guha, C;Shawber, CJ;Yan, KS;Wang, TC;
    Cellular and molecular gastroenterology and hepatology
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Organoid Culture
  6. Integrative genome-scale analyses reveal post-transcriptional signatures of early human small intestinal development in a directed differentiation organoid model
    Authors: Hung, YH;Capeling, M;Villanueva, JW;Kanke, M;Shanahan, MT;Huang, S;Cubitt, R;Rinaldi, VD;Schimenti, JC;Spence, JR;Sethupathy, P;
    BMC genomics
    Species: Mouse
    Sample Types: Organoids
  7. Vibrio cholerae biofilms use modular adhesins with glycan-targeting and nonspecific surface binding domains for colonization
    Authors: X Huang, T Nero, R Weeraseker, KH Matej, A Hinbest, Z Jiang, RF Lee, L Wu, C Chak, J Nijjer, I Gibaldi, H Yang, N Gamble, WL Ng, SA Malaker, K Sumigray, R Olson, J Yan
    Nature Communications, 2023-04-13;14(1):2104.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Fibroblast-derived EGF ligand Neuregulin-1 induces fetal-like reprogramming of the intestinal epithelium without supporting tumorigenic growth
    Authors: TT Lemmetyine, EW Viitala, L Wartiovaar, T Kaprio, J Hagström, C Haglund, P Katajisto, TC Wang, E Domènech-M, S Ollila
    Disease Models & Mechanisms, 2023-04-03;0(0):.
    Species: Mouse
    Sample Types: Organoid
    Applications: Bioassay
  9. Tellu: an object detector algorithm for automatic classification of intestinal organoids
    Authors: E Domènech-M, A Brandt, TT Lemmetyine, L Wartiovaar, TP Mäkelä, S Ollila
    Disease Models & Mechanisms, 2023-03-13;0(0):.
    Species: Mouse
    Sample Types: Organoid
    Applications: Bioassay
  10. Comprehensive microRNA analysis across genome-edited colorectal cancer organoid models reveals miR-24 as a candidate regulator of cell survival
    Authors: JW Villanueva, L Kwong, T Han, SA Martinez, MT Shanahan, M Kanke, LE Dow, CG Danko, P Sethupathy
    BMC Genomics, 2022-12-01;23(1):792.
    Species: Mouse
    Sample Types: Organoid
    Applications: Bioassay
  11. Agr2-associated ER stress promotes adherent-invasive E.�coli dysbiosis and triggers CD103+ dendritic cell IL-23-dependent ileocolitis
    Authors: M Viladomiu, M Khounlotha, B Dogan, SF Lima, A Elsaadi, E Cardakli, JG Castellano, C Ng, J Herzog, AA Schoenborn, M Ellermann, B Liu, S Zhang, AS Gulati, RB Sartor, KW Simpson, SM Lipkin, RS Longman
    Cell Reports, 2022-11-15;41(7):111637.
    Species: Transgenic Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Intestinal Epithelial Stem Cell Transplants as a Novel Therapy for Cerebrovascular Stroke
    Authors: K Kumar Mani, Y El-Hakim, TE Branyan, N Samiya, S Pandey, MT Grimaldo, A Habbal, A Wertz, F Sohrabji
    Brain, Behavior, and Immunity, 2022-10-31;0(0):.
    Species: Mouse
    Sample Types: Organoids
  13. Transplantation of intestinal organoids into a mouse model of colitis
    Authors: S Watanabe, S Kobayashi, N Ogasawara, R Okamoto, T Nakamura, M Watanabe, KB Jensen, S Yui
    Nature Protocols, 2022-02-02;0(0):.
    Species: Mouse
    Sample Types: Organoids
    Applications: Bioassay
  14. Coordinate control of basal epithelial cell fate and stem cell maintenance by core EMT transcription factor Zeb1
    Authors: Y Han, A Villarreal, G Gutierrez, Q Nguyen, P Sun, T Wu, B Sui, G Berx, T Brabletz, K Kessenbroc, YA Zeng, K Watanabe, X Dai
    Cell Reports, 2022-01-11;38(2):110240.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Rpl24Bst mutation suppresses colorectal cancer by promoting eEF2 phosphorylation via eEF2K
    Authors: JR Knight, N Vlahov, DM Gay, RA Ridgway, WJ Faller, C Proud, GR Mallucci, T von der Ha, CM Smales, AE Willis, OJ Sansom
    Elife, 2021-12-13;10(0):.
    Species: Mouse
    Sample Types: Organoid
    Applications: Bioassay
  16. Synaptotagmin-13 Is a Neuroendocrine Marker in Brain, Intestine and Pancreas
    Authors: M Tarquis-Me, K Scheibner, I González-G, A Bastidas-P, M Sterr, J Jaki, S Schirge, C García-Các, H Lickert, M Bakhti
    International Journal of Molecular Sciences, 2021-11-20;22(22):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Dietary fructose improves intestinal cell survival and nutrient absorption
    Authors: SR Taylor, S Ramsamooj, RJ Liang, A Katti, R Pozovskiy, N Vasan, SK Hwang, N Nahiyaan, NJ Francoeur, EM Schatoff, JL Johnson, MA Shah, AJ Dannenberg, RP Sebra, LE Dow, LC Cantley, KY Rhee, MD Goncalves
    Nature, 2021-08-18;0(0):.
    Species: Mouse
    Sample Types: Organoid
    Applications: Bioassay
  18. Oncogenic BRAF, unrestrained by TGF&beta-receptor signalling, drives right-sided colonic tumorigenesis
    Authors: JDG Leach, N Vlahov, P Tsantoulis, RA Ridgway, DJ Flanagan, K Gilroy, N Sphyris, EG Vázquez, DF Vincent, WJ Faller, MC Hodder, A Raven, S Fey, AK Najumudeen, D Strathdee, C Nixon, M Hughes, W Clark, R Shaw, S:CORT con, SR van Hooff, DJ Huels, JP Medema, ST Barry, MC Frame, A Unciti-Bro, SJ Leedham, GJ Inman, R Jackstadt, BJ Thompson, AD Campbell, S Tejpar, OJ Sansom
    Nature Communications, 2021-06-08;12(1):3464.
    Species: Mouse
    Sample Types: Organoid
    Applications: Bioassay
  19. Non-canonical Wnt/PCP signalling regulates intestinal stem cell lineage priming towards enteroendocrine and Paneth cell fates
    Authors: A Böttcher, M Büttner, S Tritschler, M Sterr, A Aliluev, L Oppenlände, I Burtscher, S Sass, M Irmler, J Beckers, C Ziegenhain, W Enard, AC Schamberge, FM Verhamme, O Eickelberg, FJ Theis, H Lickert
    Nature Cell Biology, 2021-01-04;23(1):23-31.
    Species: Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  20. Propionate Enhances Cell Speed and Persistence to Promote Intestinal Epithelial Turnover and Repair
    Authors: AJ Bilotta, C Ma, W Yang, Y Yu, Y Yu, X Zhao, Z Zhou, S Yao, SM Dann, Y Cong
    Cell Mol Gastroenterol Hepatol, 2020-11-22;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  21. Tp63-expressing adult epithelial stem cells cross lineages boundaries revealing latent hairy skin competence
    Authors: S Claudinot, JI Sakabe, H Oshima, C Gonneau, T Mitsiadis, D Littman, P Bonfanti, G Martens, M Nicolas, A Rochat, Y Barrandon
    Nat Commun, 2020-11-06;11(1):5645.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  22. Volumetric Compression Induces Intracellular Crowding to Control Intestinal Organoid Growth via Wnt/&beta-Catenin Signaling
    Authors: Y Li, M Chen, J Hu, R Sheng, Q Lin, X He, M Guo
    Cell Stem Cell, 2020-10-13;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  23. LSD1 represses a neonatal/reparative gene program in adult intestinal epithelium
    Authors: RT Zwiggelaar, HT Lindholm, M Fosslie, M Terndrup P, Y Ohta, A Díez-Sánch, M Martín-Alo, J Ostrop, M Matano, N Parmar, E Kvaløy, RR Spanjers, K Nazmi, M Rye, F Drabløs, C Arrowsmith, J Arne Dahl, KB Jensen, T Sato, MJ Oudhoff
    Science Advances, 2020-09-11;6(37):.
    Species: Mouse
    Sample Types: Organoid
    Applications: Bioassay
  24. Elevating EGFR-MAPK program by a nonconventional Cdc42 enhances intestinal epithelial survival and regeneration
    Authors: X Zhang, S Bandyopadh, LP Araujo, K Tong, J Flores, D Laubitz, Y Zhao, G Yap, J Wang, Q Zou, R Ferraris, L Zhang, W Hu, EM Bonder, PR Kiela, R Coffey, MP Verzi, II Ivanov, N Gao
    JCI Insight, 2020-08-20;5(16):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  25. Lineage reversion drives WNT independence in intestinal cancer
    Authors: T Han, S Goswami, Y Hu, F Tang, MP Zafra, C Murphy, Z Cao, JT Poirier, E Khurana, O Elemento, JF Hechtman, K Ganesh, R Yaeger, LE Dow
    Cancer Discov, 2020-06-16;0(0):.
    Species: Mouse
    Sample Types: Organoid
    Applications: Bioassay
  26. Targeting the Wnt signaling pathway through R-spondin 3 identifies an anti-fibrosis treatment strategy for multiple organs
    Authors: M Zhang, M Haughey, NY Wang, K Blease, AM Kapoun, S Couto, I Belka, T Hoey, M Groza, J Hartke, B Bennett, J Cain, A Gurney, B Benish, P Castiglion, C Drew, J Lachowicz, L Carayannop, SD Nathan, J Distler, DA Brenner, K Hariharan, H Cho, W Xie
    PLoS ONE, 2020-03-11;15(3):e0229445.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC Control
  27. Tight junction protein claudin-7 is essential for intestinal epithelial stem cell self-renewal and differentiation
    Authors: T Xing, LJ Benderman, S Sabu, J Parker, J Yang, Q Lu, L Ding, YH Chen
    Cell Mol Gastroenterol Hepatol, 2019-12-23;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Organoid Culture
  28. Enteroendocrine progenitor cell enriched miR-7 regulates intestinal epithelial proliferation in an Xiap-dependent manner
    Authors: AP Singh, YH Hung, MT Shanahan, M Kanke, A Bonfini, MK Dame, M Biraud, BCE Peck, OO Oyesola, JM Freund, RL Cubitt, EG Curry, LM Gonzalez, GA Bewick, ED Tait-Wojno, NA Kurpios, S Ding, JR Spence, CM Dekaney, N Buchon, P Sethupathy
    Cell Mol Gastroenterol Hepatol, 2019-11-19;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Tissue Culture
  29. Sufu- and Spop-mediated downregulation of Hedgehog signaling promotes beta cell differentiation through organ-specific niche signals
    Authors: T Yung, F Poon, M Liang, S Coquenlorg, EC McGaugh, CC Hui, MD Wilson, MC Nostro, TH Kim
    Nat Commun, 2019-10-11;10(1):4647.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  30. Wnt Signaling Protects against Paclitaxel-Induced Spiral Ganglion Neuron Damage in the Mouse Cochlea In Vitro
    Authors: X Wang, Y Han, M Wang, C Bo, Z Zhang, L Xu, W Liu, H Wang
    Biomed Res Int, 2019-10-07;2019(0):7878906.
    Species: Mouse
    Sample Types: Tissue Explants
    Applications: Tissue Culture
  31. Distinct CRC-associated APC mutations dictate response to Tankyrase inhibition
    Authors: EM Schatoff, S Goswami, MP Zafra, M Foronda, M Shusterman, BI Leach, A Katti, BJ Diaz, LE Dow
    Cancer Discov, 2019-07-23;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Tissue Culture
  32. Molecular determinants of WNT9b responsiveness in nephron progenitor cells
    Authors: KK Dickinson, LC Hammond, CM Karner, ND Hastie, TJ Carroll, P Goodyer
    PLoS ONE, 2019-04-12;14(4):e0215139.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  33. The atypical antipsychotic quetiapine induces hyperlipidemia by activating intestinal PXR signaling
    Authors: Z Meng, T Gwag, Y Sui, SH Park, X Zhou, C Zhou
    JCI Insight, 2019-02-07;4(3):.
    Species: Mouse
    Sample Types:
    Applications: Bioassay
  34. Neutrophils Promote Amphiregulin Production in Intestinal Epithelial Cells through TGF-? and Contribute to Intestinal Homeostasis
    Authors: F Chen, W Yang, X Huang, AT Cao, AJ Bilotta, Y Xiao, M Sun, L Chen, C Ma, X Liu, CG Liu, S Yao, SM Dann, Z Liu, Y Cong
    J. Immunol., 2018-08-31;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  35. Characterization of the enhancer and promoter landscape of inflammatory bowel disease from human colon biopsies
    Authors: M Boyd, M Thodberg, M Vitezic, J Bornholdt, K Vitting-Se, Y Chen, M Coskun, Y Li, BZS Lo, P Klausen, P Jan Schwei, AG Pedersen, N Rapin, K Skovgaard, K Dahlgaard, R Andersson, TB Terkelsen, B Lilje, JT Troelsen, AM Petersen, KB Jensen, I Gögenur, P Thielsen, JB Seidelin, OH Nielsen, JT Bjerrum, A Sandelin
    Nat Commun, 2018-04-25;9(1):1661.
    Species: Human
    Sample Types: Organoids
    Applications: Bioassay
  36. PAF-Myc-Controlled Cell Stemness Is Required for Intestinal Regeneration and Tumorigenesis
    Authors: MJ Kim, B Xia, HN Suh, SH Lee, S Jun, EM Lien, J Zhang, K Chen, JI Park
    Dev. Cell, 2018-03-12;44(5):582-596.e4.
    Species: Mouse
    Sample Types: Organoid
    Applications: Bioassay
  37. Monoclonal Antibodies Reveal Dynamic Plasticity Between Lgr5-�and Bmi1-Expressing Intestinal Cell Populations
    Authors: NR Smith, JR Swain, PS Davies, AC Gallagher, MS Parappilly, CZ Beach, PR Streeter, IA Williamson, ST Magness, MH Wong
    Cell Mol Gastroenterol Hepatol, 2018-03-10;6(1):79-96.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  38. The Coordinated Activities of nAChR and Wnt Signaling Regulate Intestinal Stem Cell Function in Mice
    Authors: T Takahashi, A Shiraishi, J Murata
    Int J Mol Sci, 2018-03-05;19(3):.
    Species: Mouse
    Sample Types: Complex Sample Type
    Applications: Bioassay
  39. Development of a functional thyroid model based on an organoid culture system
    Authors: Y Saito, N Onishi, H Takami, R Seishima, H Inoue, Y Hirata, K Kameyama, K Tsuchihash, E Sugihara, S Uchino, K Ito, H Kawakubo, H Takeuchi, Y Kitagawa, H Saya, O Nagano
    Biochem. Biophys. Res. Commun., 2018-02-19;0(0):.
    Species: Mouse
    Sample Types: Whole Cell
    Applications: Bioassay
  40. YAP/TAZ-Dependent Reprogramming of Colonic Epithelium Links ECM Remodeling to Tissue Regeneration
    Authors: S Yui, L Azzolin, M Maimets, MT Pedersen, RP Fordham, SL Hansen, HL Larsen, J Guiu, MRP Alves, CF Rundsten, JV Johansen, Y Li, CD Madsen, T Nakamura, M Watanabe, OH Nielsen, PJ Schweiger, S Piccolo, KB Jensen
    Cell Stem Cell, 2017-12-14;22(1):35-49.e7.
    Species: Mouse
    Sample Types: Complex Sample Type
    Applications: Bioassay
  41. Organotypic pancreatoids with native mesenchyme develop Insulin producing endocrine cells
    Authors: MA Scavuzzo, D Yang, M Borowiak
    Sci Rep, 2017-09-07;7(1):10810.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  42. Aging effects on intestinal homeostasis associated with expansion and dysfunction of intestinal epithelial stem cells
    Authors: EC Moorefield, SF Andres, RE Blue, L Van Landeg, AT Mah, MA Santoro, S Ding
    Aging (Albany NY), 2017-08-29;9(8):1898-1915.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  43. In Vitro Polarization of Colonoids to Create an Intestinal Stem Cell Compartment
    Authors: PJ Attayek, AA Ahmad, Y Wang, I Williamson, CE Sims, ST Magness, NL Allbritton
    PLoS ONE, 2016-04-21;11(4):e0153795.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  44. Convergence of cMyc and beta-catenin on Tcf7l1 enables endoderm specification.
    Authors: Morrison G, Scognamiglio R, Trumpp A, Smith A
    EMBO J, 2015-12-16;35(3):356-68.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  45. E-cadherin can limit the transforming properties of activating beta-catenin mutations.
    Authors: Huels D, Ridgway R, Radulescu S, Leushacke M, Campbell A, Biswas S, Leedham S, Serra S, Chetty R, Moreaux G, Parry L, Matthews J, Song F, Hedley A, Kalna G, Ceteci F, Reed K, Meniel V, Maguire A, Doyle B, Soderberg O, Barker N, Watson A, Larue L, Clarke A, Sansom O
    EMBO J, 2015-08-03;34(18):2321-33.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  46. Directed Differentiation of Human Embryonic Stem Cells into Prostate Organoids In Vitro and its Perturbation by Low-Dose Bisphenol A Exposure.
    Authors: Calderon-Gierszal E, Prins G
    PLoS ONE, 2015-07-29;10(7):e0133238.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  47. Selenoprotein P influences colitis-induced tumorigenesis by mediating stemness and oxidative damage.
    Authors: Barrett C, Reddy V, Short S, Motley A, Lintel M, Bradley A, Freeman T, Vallance J, Ning W, Parang B, Poindexter S, Fingleton B, Chen X, Washington M, Wilson K, Shroyer N, Hill K, Burk R, Williams C
    J Clin Invest, 2015-06-08;125(7):2646-60.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  48. R-spondin 1/dickkopf-1/beta-catenin machinery is involved in testicular embryonic angiogenesis.
    Authors: Caruso M, Ferranti F, Corano Scheri K, Dobrowolny G, Ciccarone F, Grammatico P, Catizone A, Ricci G
    PLoS ONE, 2015-04-24;10(4):e0124213.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  49. Compartmentalized accumulation of cAMP near complexes of multidrug resistance protein 4 (MRP4) and cystic fibrosis transmembrane conductance regulator (CFTR) contributes to drug-induced diarrhea.
    Authors: Moon C, Zhang W, Ren A, Arora K, Sinha C, Yarlagadda S, Woodrooffe K, Schuetz J, Valasani K, de Jonge H, Shanmukhappa S, Shata M, Buddington R, Parthasarathi K, Naren A
    J Biol Chem, 2015-03-11;290(18):11246-57.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  50. Inducible in vivo genome editing with CRISPR-Cas9.
    Authors: Dow, Lukas E, Fisher, Jonathan, O'Rourke, Kevin P, Muley, Ashlesha, Kastenhuber, Edward R, Livshits, Geulah, Tschaharganeh, Darjus F, Socci, Nicholas, Lowe, Scott W
    Nat Biotechnol, 2015-02-18;33(4):390-4.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Bioassay
  51. Bmi1 regulates murine intestinal stem cell proliferation and self-renewal downstream of Notch.
    Authors: Lopez-Arribillaga E, Rodilla V, Pellegrinet L, Guiu J, Iglesias M, Roman A, Gutarra S, Gonzalez S, Munoz-Canoves P, Fernandez-Salguero P, Radtke F, Bigas A, Espinosa L
    Development, 2014-12-05;142(1):41-50.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  52. The ErbB4 ligand neuregulin-4 protects against experimental necrotizing enterocolitis.
    Authors: McElroy S, Castle S, Bernard J, Almohazey D, Hunter C, Bell B, Al Alam D, Wang L, Ford H, Frey M
    Am J Pathol, 2014-09-09;184(10):2768-78.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  53. Small intestinal stem cell identity is maintained with functional Paneth cells in heterotopically grafted epithelium onto the colon.
    Authors: Fukuda M, Mizutani T, Mochizuki W, Matsumoto T, Nozaki K, Sakamaki Y, Ichinose S, Okada Y, Tanaka T, Watanabe M, Nakamura T
    Genes Dev, 2014-08-15;28(16):1752-7.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  54. Proinflammatory cytokine-induced tight junction remodeling through dynamic self-assembly of claudins.
    Authors: Capaldo C, Farkas A, Hilgarth R, Krug S, Wolf M, Benedik J, Fromm M, Koval M, Parkos C, Nusrat A
    Mol Biol Cell, 2014-07-16;25(18):2710-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  55. Chronic inflammation induces telomere dysfunction and accelerates ageing in mice.
    Authors: Jurk D, Wilson C, Passos J, Oakley F, Correia-Melo C, Greaves L, Saretzki G, Fox C, Lawless C, Anderson R, Hewitt G, Pender S, Fullard N, Nelson G, Mann J, van de Sluis B, Mann D, von Zglinicki T
    Nat Commun, 2014-06-24;2(0):4172.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  56. Impact of diet-induced obesity on intestinal stem cells: hyperproliferation but impaired intrinsic function that requires insulin/IGF1.
    Authors: Mah, Amanda T, Van Landeghem, Lauriann, Gavin, Hannah E, Magness, Scott T, Lund, P Kay
    Endocrinology, 2014-06-10;155(9):3302-14.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  57. Luminal microbes promote monocyte-stem cell interactions across a healthy colonic epithelium.
    Authors: Skoczek D, Walczysko P, Horn N, Parris A, Clare S, Williams M, Sobolewski A
    J Immunol, 2014-06-06;193(1):439-51.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  58. Oncogenic mutations in intestinal adenomas regulate Bim-mediated apoptosis induced by TGF-beta.
    Authors: Wiener Z, Band A, Kallio P, Hogstrom J, Hyvonen V, Kaijalainen S, Ritvos O, Haglund C, Kruuna O, Robine S, Louvard D, Ben-Neriah Y, Alitalo K
    Proc Natl Acad Sci U S A, 2014-05-13;111(21):E2229-36.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  59. beta-Catenin is required for hair-cell differentiation in the cochlea.
    Authors: Shi F, Hu L, Jacques B, Mulvaney J, Dabdoub A, Edge A
    J Neurosci, 2014-05-07;34(19):6470-9.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  60. Blockade of TLR3 protects mice from lethal radiation-induced gastrointestinal syndrome.
    Authors: Takemura N, Kawasaki T, Kunisawa J, Sato S, Lamichhane A, Kobiyama K, Aoshi T, Ito J, Mizuguchi K, Karuppuchamy T, Matsunaga K, Miyatake S, Mori N, Tsujimura T, Satoh T, Kumagai Y, Kawai T, Standley D, Ishii K, Kiyono H, Akira S, Uematsu S
    Nat Commun, 2014-03-18;5(0):3492.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  61. An enteroendocrine cell-enteric glia connection revealed by 3D electron microscopy.
    Authors: Bohorquez D, Samsa L, Roholt A, Medicetty S, Chandra R, Liddle R
    PLoS ONE, 2014-02-26;9(2):e89881.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  62. A combination of Wnt and growth factor signaling induces Arl4c expression to form epithelial tubular structures.
    Authors: Matsumoto, Shinji, Fujii, Shinsuke, Sato, Akira, Ibuka, Souji, Kagawa, Yoshinor, Ishii, Masaru, Kikuchi, Akira
    EMBO J, 2014-02-20;33(7):702-18.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  63. In vitro multilineage differentiation and self-renewal of single pancreatic colony-forming cells from adult C57BL/6 mice.
    Authors: Jin, Liang, Feng, Tao, Zerda, Ricardo, Chen, Ching-Ch, Riggs, Arthur D, Ku, Hsun Ter
    Stem Cells Dev, 2014-01-04;23(8):899-909.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  64. LGR4 and its ligands, R-spondin 1 and R-spondin 3, regulate food intake in the hypothalamus of male rats.
    Authors: Li, Ji-Yao, Chai, Biaoxin, Zhang, Weizhen, Fritze, Danielle, Zhang, Chao, Mulholland, Michael
    Endocrinology, 2013-11-26;155(2):429-40.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  65. CSF-1 receptor-dependent colon development, homeostasis and inflammatory stress response.
    Authors: Huynh D, Akcora D, Malaterre J, Chan C, Dai X, Bertoncello I, Stanley E, Ramsay R
    PLoS ONE, 2013-02-22;8(2):e56951.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  66. Colony-forming cells in the adult mouse pancreas are expandable in Matrigel and form endocrine/acinar colonies in laminin hydrogel.
    Authors: Jin L, Feng T, Shih H, Zerda R, Luo A, Hsu J, Mahdavi A, Sander M, Tirrell D, Riggs A, Ku H
    Proc Natl Acad Sci U S A, 2013-02-19;110(10):3907-12.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  67. In vivo and in vitro models for the therapeutic targeting of Wnt signaling using a Tet-ODeltaN89beta-catenin system.
    Authors: Jarde T, Evans R, McQuillan K, Parry L, Feng G, Alvares B, Clarke A, Dale T
    Oncogene, 2012-04-02;32(7):883-93.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  68. Lrig1 controls intestinal stem-cell homeostasis by negative regulation of ErbB signalling.
    Authors: Wong, Vivian W, Stange, Daniel E, Page, Mahalia, Buczacki, Simon, Wabik, Agnieszk, Itami, Satoshi, van de Wetering, Marc, Poulsom, Richard, Wright, Nicholas, Trotter, Matthew, Watt, Fiona M, Winton, Doug J, Clevers, Hans, Jensen, Kim B
    Nat Cell Biol, 2012-03-04;14(4):401-8.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Cell Culture
  69. Human RSPO1/R-spondin1 is expressed during early ovary development and augments beta-catenin signaling.
    Authors: Tomaselli S, Megiorni F, Lin L, Mazzilli MC, Gerrelli D, Majore S, Grammatico P, Achermann JC
    PLoS ONE, 2011-01-28;6(1):e16366.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

FAQs

  1. What are the differences between Recombinant Mouse R-Spondin 1 Protein, CF (Catalog # 3474-RS) and the new version, Recombinant Mouse R-Spondin 1 (CHO-expressed) Protein (Catalog # 7150-RS)?

    • Catalog # 3474-RS is an E. coli cell derived R-Spondin 1 protein with aaSer21-Gly209. The bioactivity is measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The typical ED50 is 50-200 ng/mL in the presence of 5 ng/mL recombinant mouse Wnt-3a. Additionally, the purity specification for this protein is >90%.

      Catalog # 7150-RS is a Chinese Hamster Ovary (CHO) cell derived R-Spondin 1 protein with aaSer21-Gln265. The bioactivity is measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The typical ED50 is 8-40 ng/mL in the presence of 5 ng/mL recombinant mouse Wnt‑3a. Additionally, the purity specification for this protein is >95%.

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Recombinant Mouse R-Spondin 1 Protein, CF
By Rebecca Cubitt on 11/30/2018
Application: CellProlif
Recombinant Mouse R-Spondin 1 Protein, CF
By Christine Quach on 09/08/2017
Application: Cell Culture
Recombinant Mouse R-Spondin 1 Protein, CF
By Ben McGlaughon on 09/08/2017
Application: Apoptosis assay
Recombinant Mouse R-Spondin 1 Protein, CF
By Michael Dougherty on 04/21/2017
Recombinant Mouse R-Spondin 1 Protein, CF
By Anonymous on 12/15/2016
Application: Stem/Immune cell maintenance or differentiation

Used in culture media to differentiate stem cells towards a pancreatic endocrine progenitor cell fate.