Recombinant Mouse R-Spondin 4 Protein

Catalog #: 4106-RS Datasheet / COA / SDS

Carrier Free

Catalog #	Availability	Size / Price	Qty
4106-RS-025/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
4106-RS-025

R&D Systems Recombinant Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse R-Spondin 4 Protein Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED₅₀ for this effect is 5‑20 ng/mL in the presence of 10 ng/mL Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).

Source

Chinese Hamster Ovary cell line, CHO-derived mouse R-Spondin 4 protein
Ala19-Gln228

Accession #

Q8BJ73

N-terminal Sequence
Analysis

Ala19

Predicted Molecular Mass

23.9 kDa

SDS-PAGE

32 kDa, reducing conditions

Product Datasheets

4106-RS (with carrier)

Product Datasheet

COA

SDS

4106-RS/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

4106-RS

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

4106-RS/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: R-Spondin 4

R-Spondin 4 (RSPO4, roof plate-specific spondin 4), also called cysteine-rich and single thrombospondin domain containing-4 (Cristin 4), is an ~33 kDa secreted heparin-binding protein that shares ~35% amino acid (aa) identity with three other R-Spondin family members (1-3). All are positive modulators of Wnt/ beta -catenin signaling, but R-Spondin 4 may be somewhat weaker than other R-Spondins (2). R‑Spondins regulate Wnt/ beta -catenin by competing with the Wnt antagonist DKK-1 for binding to the Wnt co-receptors LRP-6 and Kremen, reducing their DKK‑1‑mediated internalization (1, 4). Like other R‑Spondins, mouse R-Spondin 4 (234 aa) contains a signal sequence (aa 1-19), two adjacent cysteine-rich furin-like domains (aa 85-128) with one potential tyrosine phosphorylation site (aa 114), followed by a thrombospondin (TSP-1) motif (aa 137‑197) and a region rich in basic residues (aa 199‑234). The furin-like domains are sufficient for beta -catenin stabilization (2). Mature mouse R‑Spondin 4 shares 81%, 97%, 79%, 77% and 76% aa identity with human, rat, bovine, equine and canine R-Spondin 4, respectively. There is one potential isoform where Arg substitutes for the C‑terminal 82 amino acids (5). Each R‑Spondin has a distinct expression pattern (6). In the mouse, R‑Spondin 4 mRNA is found during development of limb bud mesenchyme, nail beds, heart and teeth (6‑8). In humans, mutations of R‑Spondin 4 have been found to cause anonychia, a condition in which fingernails and toenails are absent (8‑10).

References

Nam, J.-S. et al. (2006) J. Biol. Chem. 281:13247.
Kim, K.-A. et al. (2008) Mol. Biol. Cell 19:2588.
Hendrickx, M. and L. Leyns (2008) Develop. Growth Differ. 50:229.
Binnerts, M.E. et al. (2007) Proc. Natl. Acad. Sci. USA 104:14700.
Entrez Accession # Q8BJ73, Isoform 2.
Nam, J.-S. et al. (2007) Gene Expr. Patterns 7:306.
Pemberton, T.J. et al. (2007) Dev. Dyn. 236:2245.
Ishii, Y. et al. (2008) J. Invest. Dermatol. 128:867.
Blaydon, D.C. et al. (2006) Nat. Genet. 38:1245.
Bergmann, C. et al. (2006) Am. J. Hum. Genet. 79:1105.

Long Name

Roof Plate-specific Spondin 4

Entrez Gene IDs

343637 (Human); 228770 (Mouse)

Alternate Names

C20orf182; chromosome 20 open reading frame 182; Cristin 4; CRISTIN4; dJ824F16.3; FLJ16018; hRspo4; Roof plate-specific spondin-4; RSPO4; RSpondin 4; R-Spondin 4; R-spondin family, member 4; R-spondin-4