Recombinant Mouse RANK/TNFRSF11A Fc Chimera Protein, CF

Catalog #: 692-RK
4 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
692-RK-100

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Citations (4)

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse RANK/TNFRSF11A Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit TRANCE-induced osteoclast differentiation of mouse splenocytes. The ED₅₀ for this effect is 0.05‑0.15 µg/mL in the presence of 10 ng/mL of rmTRANCE.

Source

Mouse myeloma cell line, NS0-derived mouse RANK/TNFRSF11A protein

Met	Mouse RANK (Gln30 - Pro213) Accession # O35305.1	IEGRDID	Human IgG₁ (Pro100 - Lys330)	6-His tag
N-terminus				C-terminus

Accession #

O35305.1

N-terminal Sequence
Analysis

Met

Structure / Form

disulfide-linked homodimer

Predicted Molecular Mass

48 kDa (monomer)

SDS-PAGE

60 kDa, reducing conditions

Product Datasheets

692-RK

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

692-RK

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: RANK/TNFRSF11A

RANK (receptor activator of NF-kappa B, also known as TRANCE receptor, osteoclast differentiation factor receptor [ODFR] and TNFRSF11A) is a member of the tumor necrosis factor receptor family. The full length mouse RANK cDNA encodes a type I transmembrane protein of 625 amino acids (aa) with a predicted 184 aa extracellular domain and a 391 aa cytoplasmic domain. The extracellular domain contains two potential N-linked glycosylation sites. RANK shares significant amino acid homology with other members of the TNF R family in its extracellular four cysteine-rich repeats. Human and murine RANK share 81% aa identity in their extracellular domains. RANK is widely expressed with the highest levels in skeletal muscle, thymus, liver, colon, small intestine and adrenal gland. RANK is expressed in dendritic cells. In activated human peripheral blood T lymphocytes, RANK expression is induced by IL-4 and TGF-beta. Multiple tumor necrosis factor receptor-associated factors (TRAFs) are involved in the signaling of RANK. TRANCE (TNF-related activation-induced cytokines, also known as RANK ligand [RANKL], osteoprotegerin ligand [OPGL], and osteoclast differentiation factor [ODF]) is the ligand for RANK. The biological functions mediated through RANK include activation of NF-kappa B and c-jun N-terminal kinase, enhancement of T cell growth and dendritic cell function, induction of osteoclastogenesis, and lymph node organogenesis. Soluble RANK is able to block TRANCE induced biological activity.

References

Anderson, D.M. et al. (1997) Nature 390:175.
Nakagawa, N. et al. (1998) Biochem. Biophys. Res. Commun. 245:382.

Long Name

Receptor Activator of NF-kappa-B

Entrez Gene IDs

8792 (Human); 21934 (Mouse)

Alternate Names

CD265 antigen; CD265; FEO; loss of heterozygosity, 18, chromosomal region 1; ODFR; ODFROSTS; OFE; OPTB7; Osteoclast differentiation factor receptor; PDB2; RANK; RANKLOH18CR1; Receptor activator of NF-KB; receptor activator of nuclear factor-kappa B; TNFRSF11A; TRANCE R; TRANCER; tumor necrosis factor receptor superfamily member 11A; tumor necrosis factor receptor superfamily, member 11a, activator of NFKB; tumor necrosis factor receptor superfamily, member 11a, NFKB activator

Related Research Areas

Citations for Recombinant Mouse RANK/TNFRSF11A Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Development of bone seeker-functionalised microspheres as a targeted local antibiotic delivery system for bone infections
Authors: SG Rotman, K Thompson, DW Grijpma, RG Richards, TF Moriarty, D Eglin, O Guillaume
J Orthop Translat, 2019-08-30;21(0):136-145.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
OPG/RANKL/RANK axis is a critical inflammatory signaling system in ischemic brain in mice.
Authors: Shimamura M, Nakagami H, Osako M, Kurinami H, Koriyama H, Zhengda P, Tomioka H, Tenma A, Wakayama K, Morishita R
Proc Natl Acad Sci U S A, 2014-05-20;111(22):8191-6.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo
Epidermal growth factor receptor (EGFR) signaling is a key mediator of hormone-induced leukocyte infiltration in the pubertal female mammary gland.
Authors: Aupperlee M, Zhao Y, Tan Y, Leipprandt J, Bennett J, Haslam S, Schwartz R
Endocrinology, 2014-04-02;155(6):2301-13.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo
Osteoprotegerin reduces the serum level of receptor activator of NF-kappaB ligand derived from osteoblasts.
Authors: Nakamichi Y, Udagawa N, Kobayashi Y, Nakamura M, Yamamoto Y, Yamashita T, Mizoguchi T, Sato M, Mogi M, Penninger JM, Takahashi N
J. Immunol., 2007-01-01;178(1):192-200.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay