Recombinant Mouse Ryk Fc Chimera Protein, CF

Catalog #: 4649-RK Datasheet / COA / SDS

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4649-RK-050

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Recombinant Mouse Ryk Fc Chimera Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to bind biotinylated recombinant mouse Wnt-3a in a functional ELISA with an estimated K_D <50 nM.

Source

Mouse myeloma cell line, NS0-derived mouse Ryk protein

Mouse Ryk (Arg35-Arg211) Accession # Q01887	IEGRMDP	Mouse IgG_2A (Glu98-Lys330)
N-terminus		C-terminus

Accession #

Q01887

N-terminal Sequence
Analysis

Arg35

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

47 kDa (monomer)

SDS-PAGE

60-66 kDa, reducing conditions

Product Datasheets

4649-RK

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

4649-RK

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 300 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Ryk

Ryk (Related to tyrosine kinase), also called VIK in mouse, is an 70‑90 kDa type I transmembrane glycoprotein that is an atypical member of the receptor tyrosine kinase superfamily (1, 2). Mouse Ryk cDNA encodes 594 amino acids (aa) that include a 34 aa signal sequence, a 177 aa extracellular domain (ECD) that contains a WIF (Wnt Inhibitory Factor) domain (aa 50‑178), a transmembrane sequence, and a cytoplasmic region with a non‑functional Ser/Thr protein kinase domain (aa 317‑590) (1‑3). The cytoplasmic region participates in signaling by undergoing gamma ‑secretase cleavage and translocating to the nucleus in response to Wnts (4). Alternate start sites at aa 28 or aa 117 have been reported. Within the ECD, mouse Ryk shares 94% and 100% aa sequence identity with mouse and rat Ryk, respectively. Ryk protein expression is reported in developing neurons of the corticospinal tract, ventral zone, and retina, as well as adult tissue epithelia, stroma and blood vessels (4‑9). Ryk binds Wnts and EphB2/B3 receptors via its WIF domain (8‑10). It may form a Wnt receptor complex with Frizzled receptors, serving as a link between Wnt and Dishevelled and converting Wnt/Frizzled attraction signals to repulsion signals (6‑8, 10). In particular, Ryk is required for Wnt5a‑induced axon guidance after cortical axons cross the corpus callosum, and for Wnt3‑mediated guidance of developing retinal ganglion cells (7, 8). Mice deleted for the Ryk gene show defects in axon guidance that produce craniofacial defects and shortened limbs (10, 11). Ryk^-/- mice share a cleft palate phenotype with EphB2/B3‑deleted mice (10). Ryk is often over‑expressed in human ovarian cancer (5).

References

Hovens, C.M. et al. (1992) Proc. Natl. Acad. Sci. USA 89:11818.
Clark, C.E.J. et al. (2012) Neurosignals 20:202.
Katso, R.M. et al. (1999) Mol. Cell. Biol. 19:6427.
Lyu, J. et al. (2008) Dev. Cell 15:773.
Katso, R.M. et al. (1999) Cancer Res. 59:2265.
Liu, Y. et al. (2005) Nat. Neurosci. 8:1151.
Schmitt, A.M. et al. (2006) Nature 439:31.
Keeble, T.R. et al. (2006) J. Neurosci. 26:5840.
Kamitori, K. et al. (2005) Biochem. Biophys. Res. Commun. 330:446.
Lu, W. et al. (2004) Cell 119:97.
Halford, M.M. et al. (2000) Nat. Genet. 25:414.

Long Name

Receptor Type Tyrosine Protein Kinase RYK

Entrez Gene IDs

6259 (Human); 20187 (Mouse); 140585 (Rat)

Alternate Names

D3S3195; EC 2.7.10; EC 2.7.10.1; hydroxyaryl-protein kinase; JTK5; JTK5A protein tyrosine kinase; JTK5A; RYK receptor-like tyrosine kinase; Ryk; RYK1; tyrosine-protein kinase RYK